First Survey on the Presence and Distribution of Oxytetracycline-Resistance Genes in Anaplasma Species

Abstract

Introduction

Anaplasma sp. is an obligatory intracellular Gram-negative tick-transmitted bacterial pathogen of humans and animals. Oxytetracycline and chlortetracycline are the drugs of choice for treating domestic animals with acute anaplasmosis. Lack of documented information about oxytetracycline resistance in Anaplasma species in the world was the scope of this study to screen by PCR for the detection of the oxytetracycline-resistance genes in Anaplasma species from infected cattle and sheep in the Northwest and Southwest of Iran.

Materials and Methods

Total of 100 cattle and sheep blood samples collected from 2 provinces in the Northwest and 1 province in the Southwest of Iran were tested microscopically by the Giemsa staining examination and confirmed by PCR. Then the presence of two different oxytetracycline-resistance genes (otrA, and otrB) was detected by PCR in positive samples.

Results

The results showed that 60% of Anaplasma-infected samples were identified to have an otrA-resistance gene, and 26.67% had an otrB-resistance gene. The coexistence of two oxytetracycline-resistance determinants was encountered in 13.33% of the isolates. The significant difference in the frequency of otr genes was found among three Anaplasma species (A. marginale, A. centrale and A. ovis), and among three studied regions in Iran (p < 0.05). The identified sequences were submitted to the GenBank and deposited under accession numbers MN880729 and MN895439 for otrB and otrA genes.

Conclusion

This study, for the first time, indicated the oxytetracycline-resistance genes in the three most prevalent Anaplasma species in ruminants. This finding helps to select an appropriate treatment strategy for eradication of anaplasmosis.

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Acknowledgements

The authors are grateful to University of Tabriz (Grant no. 433296787) for financial supporting and to Katiraee. F for assistance in the submission of the otr genes in the GenBank.

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Correspondence to Parisa Shahbazi.

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This study was undertaken by bioethics committee of University of Tabriz https://lib.tabrizu.ac.ir/fa/page/8435/bioethics and accomplished according to the guidelines for the care and use of animals in research by https://ethics.research.ac.ir/.

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Shahbazi, P., Nouri Gharajalar, S., Mohebbi, K. et al. First Survey on the Presence and Distribution of Oxytetracycline-Resistance Genes in Anaplasma Species. Acta Parasit. (2020). https://doi.org/10.1007/s11686-020-00306-y

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Keywords

  • Anaplasma
  • Oxytetracycline
  • Drug resistance
  • Genes
  • Livestock
  • Iran