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First Description of the Metacercaria of Nematostrigea serpens serpens (Nitzsch, 1819) (Trematoda, Strigeidae) and Phylogenetic Affiliation of Nematostrigea vietnamiensis Zhokhov & Mishina, 2011

Abstract

Background

The genus Nematostrigea comprises trematodes parasitising in fish-eating birds of Europe, Africa, and North America. Their life cycle is unknown.

Purpose

To provide the first description of metacercariae of Nematostrigea serpens serpens, a nominative subspecies of the type species of Nematostrigea, and to record metacercaria of Nematostrigea vietnamiensis, with molecular data.

Methods

Encysted metacercariae of N. serpens serpens and N. vietnamiensis were collected from fish in Russia and Vietnam, respectively, and were processed, identified, and documented using standard morphological techniques. The 28S rRNA gene of metacercariae of both species and the cox1 gene of metacercariae an earlier studied adult of N. serpens serpens were sequenced and used for phylogenetic analysis.

Results

Metacercaria of N. serpens serpens have a spatulate body with a foliate forebody and a short hindbody, two long lateral rectilinear pseudosuckers, and the holdfast organ with bifurcated anterior and entire posterior external lobes. The analysis of the cox1and 28S rRNA gene sequences unequivocally showed the conspecificity of metacercariae and adult stage of N. serpens serpens. Based on the 28S rRNA gene sequence-based phylogenetic analysis, the genus Nematostrigea is a sister taxon to the group of strigeid genera Cotylurus + Ichthyocotylurus. Morphological and phylogenetic data demonstrated that N. vietnamiensis does not belong to the genus Nematostrigea and is possibly be affiliated with the crassiphialine trematodes.

Conclusion

This is the first record of metacercariae of N. serpens serpens. N. vietnamiensis, renamed Prodiplostomulum vietnamiense comb. nov., must be moved to the crassiphialine ‘Prodiplostomulum-type metacercariae group

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Acknowledgements

The authors wish to thank Dr Alexey Tiunov (IPEE RAS), Boris Efeykin (Center of Parasitology IPEE RAS), Drs Igor Palko, Irina Semenyuk, Anna Vasil’eva (Russian-Vietnamese Tropical Center), and Drs Alexey Parshukov, Nikolay Ilmast and Yaroslav Kuchko (Institute of Biology, Karelian Research Centre of the RAS) for help with the sampling.

Funding

The work was supported by Russian-Vietnamese Tropical Center and the state order 0218-2019-0075.

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Sokolov, S.G., Lebedeva, D.I., Khasanov, F.K. et al. First Description of the Metacercaria of Nematostrigea serpens serpens (Nitzsch, 1819) (Trematoda, Strigeidae) and Phylogenetic Affiliation of Nematostrigea vietnamiensis Zhokhov & Mishina, 2011. Acta Parasit. 66, 664–672 (2021). https://doi.org/10.1007/s11686-020-00275-2

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Keywords

  • Channa
  • Esox
  • Prodiplostomulum
  • Crassiphialinae
  • Karelia
  • Vietnam
  • 28S rRNA
  • cox1