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First Molecular Detection of Dirofilaria immitis and D. repens in Dogs from Kyrgyzstan

Acta Parasitologica volume 65, pages 949–953 (2020)Cite this article

Abstract

Background

Dirofilaria immitis and Dirofilaria repens are the causative agents of cardiopulmonary and subcutaneous dirofilariosis, respectively. This neglected disease mainly seen in dogs, cats and wild carnivores is re-emerging recent years. No study was conducted on dirofilariosis in dogs in Kyrgyzstan.

Purpose

The goal of this study was to investigate Dirofilaria species using PCR and sequencing in dogs from Kyrgyzstan.

Method

Dirofilaria spp. infection in dogs was screened via convential PCR and sequencing in 337 dogs from Kyrgyzstan.

Result

The overall prevalence of Dirofilaria spp. was 0.59% (2/337): DNA of D. immitis was detected in one sample and DNA of D. repens in second positive sample. In second sample, parallel co-infection of D. repens with Wolbachia was also found. While D. immitis sequence showed 98.70–100% similarity with previously reported sequences of D. immitis from dog blood, D. repens shared 100% identity with other sequences of D. repens.

Conclusion

These results provided first evidence for Dirofilaria spp. in Kyrgyzstan and emphasized the veterinary and medical importance.

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Acknowledgements

The authors thank Dr. Viktória Čabanová (Slovak Academy of Sciences, Institute of Parasitology, Košice 040 01, Slovakia) for providing positive control DNA of D. repens. Also, we are grateful to animal shelter staffs and Kyrgyz-Turkish Manas University Veterinary Teaching Hospital staffs for their kind help during sample collection.

Author information

Affiliations

  1. Department of Public Health, Faculty of Health Sciences, University of Karamanoglu Mehmetbey, 70100, Karaman, Turkey

    Mehmet Fatih Aydın

  2. Department of Parasitology, College of Veterinary Medicine, Sivas Cumhuriyet University, 58140, Sivas, Turkey

    Kürşat Altay

  3. College of Veterinary Medicine, Kırgızistan-Türkiye Manas University, 720044, Bishkek, Kyrgyzstan

    Ayperi Aytmirzakizi & Nazir Dumanlı

  4. Department of Parasitology, College of Veterinary Medicine, Firat University, 23119, Elazig, Turkey

    Nazir Dumanlı

Authors
  1. Mehmet Fatih Aydın
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  2. Kürşat Altay
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  3. Ayperi Aytmirzakizi
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  4. Nazir Dumanlı
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Corresponding author

Correspondence to Mehmet Fatih Aydın.

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The authors declare that they have no conflicts of interest.

Ethic statement

Ethics committee approval was received from the Animal Experimentation Ethics Committee of Kyrgyz-Turkish Manas University (Document No: 29.06.2017/2017-06/01).

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Cite this article

Aydın, M.F., Altay, K., Aytmirzakizi, A. et al. First Molecular Detection of Dirofilaria immitis and D. repens in Dogs from Kyrgyzstan. Acta Parasit. 65, 949–953 (2020). https://doi.org/10.1007/s11686-020-00245-8

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  • DOI: https://doi.org/10.1007/s11686-020-00245-8

Keywords

  • Dirofilaria immitis
  • Dirofilaria repens
  • Kyrgyzstan
  • Dog
  • Vector-borne diseases
  • PCR
  • Sequencing