The Nematodes Thelazia gulosa Railiet and Henry, 1910 and Thelazia skrjabini Erschov, 1928 as a Cause of Blindness in European Bison (Bison bonasus) in Poland

Abstract

Purpose

The nematodes of the genus Thelazia are the cause of eye diseases of wild and domestic ruminants throughout the world. The aim of the study was to describe clinical cases of thelasiosis in European bison (Bison bonasus) in Poland, and provide morphometrical features of Thelazia gulosa Railiet and Henry, 1910 and Thelazia skrjabini Erschov, 1928 regarded as potentially useful for species differentiation

Methods

The conjunctival sacs, tear ducts, the surface of the cornea and nicitating membrane collected from bison were rinsed with saline solution. Any nematodes isolated from the sediment were subjected to morphometric analysis.

Results

Thirteen of the 16 examined European bison were infected with Thelazia nematodes, belonging to the species T. gulosa and T. skrjabini. The intensity of infection ranged from one to six (mean intensity 5), and four to 29 (mean intensity 14) nematodes T. skrjabini and T. gulosa respectively. Congestion of conjunctival sac, keratitis and corneal opacity, corneal ulceration and perforation as well as purulent eyeball inflammation were observed in infected animals.

Conclusions

Thelazia gulosa and T. skrjabini can be identified by morphometrical features. As thelasiosis might be a serious threat for protected population of European bison, further studies are needed of the epidemiology and pathology of this emerging parasitosis in Poland.

Introduction

The European bison (Bison bonasus) is the largest mammalian herbivore in Europe. Although it became extinct in the wild at the beginning of XX century, the species was successfully restored from captive animals and reintroduced to a number of countries in central and eastern Europe [1]. Currently, there are about 7500 European bison worldwide and over 24% of their population inhabit Poland, which remains the center of European bison breeding [2, 3]. However, the European bison remains an endangered and rare species, demanding regular health monitoring. The biggest threat for the population of European bison is presented by infectious diseases, including parasitoses [4,5,6]. Parasitological studies of European bison in Poland have been performed since the 1950s [7, 8]; however, climate change, animal migrations and the growth of the European bison population constantly provoke the spread of new emerging parasitoses. Recently, an increasing number of clinical cases of thelasiosis in European bison has been observed in Poland (Stanisław Kaczor, pers. commun.).

Nematodes of the genus Thelazia (Spirurida: Thelaziidae) are agents of eye diseases of domestic and wild ruminants in Europe, Asia, Africa, North America and Australia. The genus Thelazia is represented by five species in Europe: Thelazia gulosa Railiet and Henry, 1910, T. rhodesi Desmarest, 1827, T. skrjabini Erschov, 1928, T. lacrymalis Gurlt, 1831 and T. callipaeda Railliet and Henry, 1910. The nematodes infect cattle (Bos taurus), zebu (Bos indicus), European and American bison (Bison bison) and buffalo (Syncerus caffer), where they localize in the conjunctival sac and tear ducts, as well as beneath the nictitating membrane and on the cornea. T. rhodesi was also isolated from the eyeball of a horse (Equus caballus) [9] and T. callipeda from wild and domestic carnivores [10]. In addition, T. gulosa infection was also confirmed in a human in the United States of America [11, 12].

Flies from the genus Musca, including M. amica, M. autumnalis, M. larvipara, M. osiris, M. vitripennis and M. hervei are considered as intermediate hosts of Thelazia nematodes [9, 13, 14]. Mature female nematodes produce numerous larvae in the conjunctival sac of the definitive host. The larvae enter flies, the intermediate host, as they are licking the secretions from the eye of the host. In the fly, the larvae develop into their invasive stage by moulting twice, and then migrate to the fly suckers. From here, they are passed into the conjunctival sac of the next definitive host while the fly is licking the secretion from the eye of the ruminant [9, 15].

The pathogenic effect of nematodes of the genus Thelazia in cattle derives from the mechanical irritation of the conjunctiva and cornea, as well as the toxic effect of parasite metabolites. The infected animals suffer from acute conjunctivitis, tearing, swelling and increased eyelid temperature, conjunctival congestion and photophobia, as well as the production of serous and mucous exudates, which turn into purulence and cause eyelid clumping. This is followed by corneal opacity and ulceration with secondary bacterial infections, leading to purulent eye inflammation. Lesions in eyeballs are accompanied by general clinical signs, e.g. lack of appetite, indigestion and emaciation [16, 17].

The aim of the study was to describe cases of thelasiosis in European bison in Poland and to perform a morphometrical analysis of the nematodes T. gulosa and T. skrjabini to identify potentially differentiating features.

Materials and Methods

Sixteen European bison, aged three to 20 years, were included in the study; all had died in the years 2018–2020 due to various reasons, such as weakness, skin disorders and balanopostitis. All bison came from free-living populations in the Białowieża Primeval Forest (11 animals) and the Knyszyn Forest (one animal) in north-eastern Poland as well as the Bieszczady Mountains (four animals) in southern Poland. Five of the bison in the Białowieża Primeval Forest and three in the Bieszczady Mountains were culled due to visible changes in eyeballs. The eyeballs of the examined bison, together with adjacent tissues, were delivered to the laboratory of the Witold Stefański Institute of Parasitology, Polish Academy of Sciences and subjected to parasitological examination. The conjunctival sacs, tear ducts, the surface of the cornea and the nictitating membrane were rinsed with physiological solution. The eyeballs were dissected and their structures thoroughly rinsed. The decantated sediment was examined under the stereoscopic microscope on the presence of nematodes. Any obtained parasites were isolated and stored in 70% alcohol with 5% glycerol for further identification. Morphometrical analysis of the nematodes was performed using an Olympus BX50 light microscope (Olympus, Japan) with a Cell D digital image analysis system.

Results

Nematodes from the genus Thelazia were identified in 13 of the 16 examined European bison, i.e. a prevalence of 81.2%. Nematodes belonging to the species T. gulosa and T. skrjabini were found in the conjunctival sacs, tear ducts, under the nictitating membrane and on the surface of the cornea. Nine of the infected European bison came from the Białowieża Primeval Forest. Four animals were infected with T. gulosa and another four with T. skrjabini. One bison was co-infected with both Thelazia spp., isolated from the same eyeball: the animal was culled due to emaciation and blindness, caused by cataract and corneal ulceration. In the Knyszyn Forest, T. gulosa nematodes were isolated from one European bison. In the Bieszczady Mountains, three animals were infected with T. skrjabini. The intensity of infection ranged from one to six nematodes with mean intensity reaching five for T. skrjabini and four to 29 with mean intensity 14 for T. gulosa. The highest infection intensity was observed in a European bison from the Białowieża Primeval Forest: a 17-year-old male, culled due to skin disorders.

Five infected European bison, more specifically four from the Białowieża Primeval Forest and one from the Knyszyn Forest, did not show clinical signs of thelasiosis, and no pathological changes were found in their eyeballs during necropsy. However, numerous gross lesions were observed in the eyeballs of eight infected animals, including all three European bison examined in the Bieszczady Mountains and five from the Białowieża Primeval Forest: pathological changes included hyperemia and keratitis of the conjunctival sac, and its opacity, cataract, corneal ulceration and purulent ocular inflammation, as well as perforation of corneal ulcers.

Morphometrical Description of T. gulosa

White-yellowish nematodes are rounded at the anterior end and pointed at the posterior one (Fig. 1). The body is covered with a cuticle with pronounced transverse striation. The lipless mouth has a well-developed buccal capsule. The opening of the mouth was surrounded by four papillae and two amphids; the mouth enters into a cylindrical pharynx, which slightly widens before entering the intestine.

Fig. 1
figure1

Morphometrical characteristics for the differential diagnosis of Thelazia gulosa (ac) and Thelazia skrjabini (df). a and d—anterior end of the female; b and e—posterior end of the female; c and f—posterior end of the male

Male: Body length from 6.6 to 9.8 mm, maximum width 275–489 µm, buccal capsule width 29–47 µm and buccal capsule depth 20–33 µm. The length of the cylindrical pharynx 275–380 µm, the width 69–83 µm. The nerve ring is located about 156–261 µm from the anterior end of the body. The body is 91–104 µm wide at the area of the cloaca. Two spicules are located at the posterior end of the body: the right spicule is shorter than the left and slightly bent, i.e. 132–163 µm long and 9–20 µm wide, while the left spicule is much longer and thinner, i.e. 745–897 µm long and 6–9 µm wide. The cloaca is located 89–104 µm from the posterior end of the body; 10 to 24 papillae are located behind the cloaca, and three pairs in front of it. The posterior end of the body is ventrically bent and pointed. The tail cuticle has two small sensilia (phasmids).

Female: The body ranges from 8.3 to 14.9 mm in length, maximum width 375–585 µm. The buccal capsule is 40–55 µm wide, and 16–41 µm deep. The cylindrical pharynx is 369–425 µm long and 73–106 µm wide. The nerve ring is located about 231–254 µm from the anterior end of the body. The genital pore is about 564–715 µm from the anterior end of the body and the anal pore 121–174 µm. from the posterior end.

Morphometrical Description of T. skrjabini

The nematodes are thin and yellow with rounded, tapered ends (Fig. 1). The cuticle shows very delicate, barely visible striation. The mouth opens terminally, surrounded by two rows of papillae: the internal row consists of six small papillae located directly around the mouth, and the external row consists of four larger papillae and two amphids. The mouth leads to a small buccal capsule, maximum width 22–25 µm and maximum depth 7–8 µm.

Male. The body is 7.1–9.2 mm long and 197–211 µm wide. The pharynx is 325–386 µm long and 39–46 µm wide. The nerve ring is located about 165–192 µm from the anterior end of the body. The posterior end of the nematode is bent and equipped with two spicules; both are 8–10 µm wide, however, the right is 92–12.1 µm long and the left is 130–176 µm long. The cloaca is located 82–92 µm from the posterior end of the body. In addition, 15–30 pairs of papillae are located behind the cloaca and only three pairs in front.

Female. The body is 10.8–15.9 mm long and 235–282 µm wide. The pharynx is 312–367 µm long and 51–65 µm wide. The nerve ring is located about 171–217 µm from the anterior end of the body. The genital pore is about 474–625 µm from the anterior end of the body. The anal pore is located 91–97 µm. from the posterior end of the body. The tail is 85–93 µm long. A pair of phasmids are present at the posterior end of the nematode.

Discussion

Little data currently exists about the occurrence of nematodes from the genus Thelazia in ruminants in Europe. However, infection of bovines with nematodes belonging to the species T. gulosa and T. skrjabini was commonly diagnosed between the 1940s and 1960s in many regions of Poland. The prevalence of nematodes ranged from 4.4 to 25% of examined domestic ruminants. Thelasiosis was also a cause of keratoconjunctivitis in some bovine individuals; however, the intensity of infection was low, not exceeding 15 nematodes [15,16,17,18,19,20,21,22,23,24,25].

Although thelasiosis had commonly been observed in bovines in Poland, only few studies have examined the infection of wild ruminants. During the years of European bison restitution, no clinical signs of parasitosis were observed in either captive or free-living animals. The first report of T. gulosa and T. skrjabini in European bison in Poland comes from 1954–1957 [7, 8]. The mentioned study showed that 16% of examined animals were infected with T. skrjabini and 12% with T. gulosa, with the infected European bison coming from the Białowieża Primeval Forest, Niepołomice Forest, Pszczyna and zoological garden in Płock.

In later years (1983–1986), T. gulosa nematodes were found in two out of seven examined bison in the Białowieża Primeval Forest and the intensity of infection reached only one or two nematode specimens [26]. However, no clinical signs of thelasiosis were observed in European bison examined in the 1950s and 1980s, despite them being infected with the parasite. The very first clinical case of thelasiosis was published only recently, in 2013, in European bison from the Bieszczady Mountains. Infection with nematode T. gulosa was a cause of serious pathological lesions, including bilateral opacity and erosions of the cornea, as well as lens damage and purulent eye inflammation, leading to the culling of the infected animal due to blindness [27].

In 2019, clinical signs of thelasiosis were observed in several bison from the Bieszczady Mountains (Stanisław Kaczor, pers. commun.) and from the Białowieża Primeval Forest (Michał Krzysiak, pers. commun.). Over 80% of European bison from three different locations, examined in the present study, were infected with T. gulosa and/or T. skrjabini. Even though the infection was accompanied by distinct, visible clinical signs in eight animals, another five infected individuals did not demonstrate any signs of the disease. Hence, the problem of thelasiosis is probably more widespread than can be determined just by observing European bison in the wild, as accurate laboratory analysis is needed to detect subclinical cases of infected animals. Presumably, the animals with no disease are at an early stage of the disease; however, they may still be a source of infection for other individuals.

It is difficult to speculate about the reason for the increasing number of clinical cases of thelasiosis observed in European bison in Poland. The transmission of the disease in the environment occurs through flies which act as an intermediate host of Thelazia nematodes; such transmission enables significant promotion of parasite spread and complicates any control strategies, compared to parasites with a simple life cycle, e.g. blood-sucking nematode Ashworthius sidemi (Strongylida; Trichostrongylidae). In case of A. sidemi, the highest infection intensities have been recorded in the largest bison herds, where the winter supplementary feeding of European bison is common [4]. Therefore, it may be possible to the suppress the spread of the parasite by modifying European bison management practices. Furthermore, it cannot be ruled out that higher animal densities also favour Thelazia spp. transmission, as previously shown for domestic cattle [23, 24].

In addition, it might be possible that the transmission is facilitated by the migration of European bison and their utilization of fields and meadows outside the forest; the same places which are grazed by domestic cattle. Currently, around 250 European bison live permanently outside the complex of the Białowieża Primeval Forest and graze on fields together with domestic ruminants (Michał Krzysiak, pers. commun.). Unfortunately, there is no recent data about the prevalence of thelasiosis in domestic ruminants in Poland; however, it should not be excluded that infected cattle may be one of the possible sources of this parasitosis for European bison. Moreover, it cannot be ruled out that climate change may have an impact on increasing parasite transmission and infectivity [28]. Flies, intermediate hosts for Thelazia transmission, are probably present in greater numbers and for longer durations during the year when mean annual temperatures are higher and winters are milder.

The morphometrical description of the nematodes isolated from the European bison in the present study corresponds with those given by other authors [8, 29, 30] and indicates the presence of two Thelazia spp. species—T. gulosa and T. skrjabini. However, all Thelazia spp. species exhibit the same pathogenicity [29]. Therefore, the anathomopathological changes observed in the examined European bison are similar to those described in cattle by other authors [16, 17, 25]. Extremely intensive Thelazia spp. infection in bovine may lead to the formation of granulomas on the conjunctival sac, together with inflammatory infiltration [30]; however, no such lesions were observed in European bison in the present study, possibly due to the low infection intensity.

Conclusions

To conclude, the presence of nematodes from the genus Thelazia in the three examined wild populations of European bison suggests that the parasite may be a serious threat for this endangered species, especially when clinical signs occur. The recent increase in observations of thelasiosis in European bison is disturbing and demands regular monitoring, not only because of the high possibility of transmission of the disease to other ruminants. Further research on the epidemiology and pathology of this emerging disease in populations of both wild and domestic ruminants in Poland and other European countries is needed.

Data availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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The author(s) received no financial support for the research, authorship, and/or publication of this article.

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AWD and KFH: concept of the manuscript, writing of the manuscript, isolation of parasites, performing morphometrical analysis; BM: concept and editing of the manuscript; AG: isolation of parasites, morphometrical analysis; ZL and AWM: editing of the manuscript, data collection; MKS and MK: collection and preservation of the material, editing of the manuscript; SK and EPK: collection and preservation of the material.

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Correspondence to Katarzyna Filip-Hutsch.

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Demiaszkiewicz, A.W., Moskwa, B., Gralak, A. et al. The Nematodes Thelazia gulosa Railiet and Henry, 1910 and Thelazia skrjabini Erschov, 1928 as a Cause of Blindness in European Bison (Bison bonasus) in Poland. Acta Parasit. (2020). https://doi.org/10.1007/s11686-020-00243-w

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Keywords

  • Bison bonasus
  • Morphometric analysis
  • Parasitic infections
  • Thelazia spp.