Cyclospora cayetanensis infections among diarrheal outpatients in Shanghai: a retrospective case study
Cyclospora cayetanensis is a foodborne and waterborne pathogen that causes endemic and epidemic human diarrhea worldwide. A few epidemiological studies regarding C. cayetanensis infections in China have been conducted. During 2013, a total of 291 stool specimens were collected from patients with diarrhea at a hospital in urban Shanghai. C. cayetanensis was not detected in any of the stool specimens by traditional microscopy, whereas five stool specimens (1.72%, 5/291) were positive by PCR. These positive cases confirmed by molecular technology were all in the adult group (mean age 27.8 years; 2.94%, 5/170) with watery diarrhea. Marked infection occurred in the rainy season of May and July. Sequence and phylogenetic analyses of the partial 18S rRNA genes of C. cayetanensis isolated showed intra-species diversity of this parasite. This study showed, for the first time, that C. cayetanensis is a pathogen in outpatients with diarrhea in Shanghai, albeit at a low level. However, the transmission dynamics of this parasite in these patients remain uncertain.
KeywordsCyclospora cayetanensis outpatients with diarrhea stool specimens 18S rRNA gene
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We would like to thank Professor Longxian Zhang at Henan Agricultural University for his helpful advice on morphological identification of Cyclospora. This work was supported by grants from the Chinese Special Program for Scientific Research of Public Health (No. 201302004 to Y.S., No. 201502021 to J.C.), the National Key Research and Development Program of China (No. 2016YFC1201900 to J.C.) and the Fourth Round of Three-Year Public Health Action Plan of Shanghai, China (No. 15GWZK0101 to J.C.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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