Associations between Mycobacterium tuberculosis Beijing genotype and drug resistance to four first-line drugs: a survey in China
Investigations on the genetic diversity of Mycobacterium tuberculosis in China have shown that Beijing genotype strains play a dominant role. To study the association between the M. tuberculosis Beijing genotype and the drug-resistance phenotype, 1286 M. tuberculosis clinical isolates together with epidemiological and clinical information of patients were collected from the center for tuberculosis (TB) prevention and control or TB hospitals in Beijing municipality and nine provinces or autonomous regions in China. Drug resistance testing was conducted on all the isolates to the four first-line anti-TB drugs (isoniazid, rifampicin, streptomycin, and ethambutol). A total of 585 strains were found to be resistant to at least one of the four anti-TB drugs. The Beijing family strains consisted of 499 (53.20%) drug-sensitive strains and 439 (46.80%) drug-resistant strains, whereas the non-Beijing family strains comprised 202 (58.05%) drug-sensitive strains and 146 (41.95%) drug-resistant strains. No significant difference was observed in prevalence (χ2= 2.41, P > 0.05) between the drug-resistant and drugsensitive strains among the Beijing family strains. Analysis of monoresistance, multidrug-resistant TB, and geographic distribution of drug resistance did not find any relationships between the M. tuberculosis Beijing genotype and drug-resistance phenotype in China. Results confirmed that the Beijing genotype, the predominant M. tuberculosis genotype in China, was not associated with drug resistance.
Keywordstuberculosis drug resistance genotype molecular biology
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This work was funded by the projects of the National Key Program of Mega Infectious Disease (Nos. 2013ZX10003006-002 and 2013ZX10003002-001) and the project of the State Key Laboratory for Infectious Disease Prevention and Control (No. 2011SKLID- 2008). The funders had no role in the study design, data collection and analysis, manuscript preparation, or decision to publish.We also thank the staff of the respective institutes in Beijing municipality, the 10 provinces, and autonomous regions in China for their excellent contribution to this study, especially for the help of Lishui Zhang (Fujian), Yunhong Tan (Hunan), Xiujun Yang (Jilin), Chongxiang Tong (Gansu), Feiying Liu (Guangxi), Yingcheng Qi (Xinjiang), Li Shi (Tibet), Xiaohui Cao (Beijing), Haitao Li (Henan), and Jun Yang (Sichuan).
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