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Frontiers of Medicine

, Volume 10, Issue 4, pp 465–472 | Cite as

Distinct roles of ASIC3 and TRPV1 receptors in electroacupuncture-induced segmental and systemic analgesia

  • Juanjuan Xin
  • Yangshuai Su
  • Zhaokun Yang
  • Wei He
  • Hong Shi
  • Xiaoyu Wang
  • Ling Hu
  • Xiaochun Yu
  • Xianghong Jing
  • Bing Zhu
Research Article

Abstract

Previous studies have demonstrated the effects of different afferent fibers on electroacupuncture (EA)-induced analgesia. However, contributions of functional receptors expressed on afferent fibers to the EA analgesia remain unclear. This study investigates the roles of acid-sensing ion channel 3 (ASIC3) and transient receptor potential vanilloid 1 (TRPV1) receptors in EA-induced segmental and systemic analgesia. Effects of EA at acupoint ST36 with different intensities on the C-fiber reflex and mechanical and thermal pain thresholds were measured among the ASIC3–/–, TRPV1–/–, and C57BL/6 mice. Compared with C57BL/6 mice, the ipsilateral inhibition of EA with 0.8 C-fiber threshold (0.8Tc) intensity on C-fiber reflex was markedly reduced in ASIC3–/– mice, whereas the bilateral inhibition of 1.0 and 2.0Tc EA was significantly decreased in TRPV1–/– mice. The segmental increase in pain thresholds induced by 0.3 mA EA was significantly reduced in ASIC3–/– mice, whereas the systemic enhancement of 1.0 mA EA was markedly decreased in TRPV1–/– mice. Thus, segmental analgesia of EA with lower intensity is partially mediated by ASIC3 receptor on Aβ-fiber, whereas systemic analgesia induced by EA with higher intensity is more likely induced by TRPV1 receptor on Aδ- and C-fibers.

Keywords

electroacupuncture analgesia ASIC3 TRPV1 C-fiber reflex 

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Copyright information

© Higher Education Press and Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Juanjuan Xin
    • 1
  • Yangshuai Su
    • 1
  • Zhaokun Yang
    • 1
  • Wei He
    • 1
  • Hong Shi
    • 1
  • Xiaoyu Wang
    • 1
  • Ling Hu
    • 1
  • Xiaochun Yu
    • 1
  • Xianghong Jing
    • 1
  • Bing Zhu
    • 1
  1. 1.Institute of Acupuncture and MoxibustionChina Academy of Chinese Medical SciencesBeijingChina

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