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Effects of miR-200c on the migration and invasion abilities of human prostate cancer Du145 cells and the corresponding mechanism

Frontiers of Medicine volume 8, pages 456–463 (2014)Cite this article

Abstract

microRNAs (miRNAs) have played a key role in human tumorigenesis, tumor progression, and metastasis. On the one hand, miRNAs are aberrantly expressed in many types of human cancer; on the other hand, miRNAs can function as tumor suppressors or oncogenes that target many cancer-related genes. This study aimed to investigate the effects of miRNA-200c (miR-200c) on the biological behavior and mechanism of proliferation, migration, and invasion in the prostate cancer cell line Du145. In this study, Du145 cells were transfected with miR-200c mimics or negative control miR-NC by using an X-tremeGENE siRNA transfection reagent. The relative expression of miR-200c was measured by RT-PCR. The proliferation, migration, and invasion abilities of Du145 cells were detected by CCK8 assays, migration assays and invasion assays, respectively. The expressions of ZEB1, E-cadherin, and vimentin were observed by western blot. Results showed that DU145 cells exhibited a high expression of miR-200c compared with immortalized normal prostate epithelial cell RWPE-1. Du145 cells were then transfected with miR-200c mimics and displayed lower abilities of proliferation, migration, and invasion than those transfected with the negative control. The protein levels of ZEB1 and vimentin were expressed at a low extent in Du145 cells, which were transfected with miR-200c mimics; by contrast, E-cadherin was highly expressed. Hence, miR-200c could significantly inhibit the proliferation of the prostate cancer cell line Du145; likewise, miR-200c could inhibit migration and invasion by epithelial-mesenchymal transition.

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Affiliations

  1. Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Runlin Shi, Haibing Xiao, Tao Yang, Lei Chang, Yuanfeng Tian, Bolin Wu & Hua Xu

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  1. Runlin Shi
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  2. Haibing Xiao
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  3. Tao Yang
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  4. Lei Chang
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  5. Yuanfeng Tian
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  6. Bolin Wu
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  7. Hua Xu
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Correspondence to Hua Xu.

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Shi, R., Xiao, H., Yang, T. et al. Effects of miR-200c on the migration and invasion abilities of human prostate cancer Du145 cells and the corresponding mechanism. Front. Med. 8, 456–463 (2014). https://doi.org/10.1007/s11684-014-0353-z

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  • DOI: https://doi.org/10.1007/s11684-014-0353-z

Keywords

  • miR-200c
  • proliferation
  • migration
  • invasion
  • prostate cancer
  • Du145 cell
  • ZEB1