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Nucleic acid-based diagnostics for infectious diseases in public health affairs

Frontiers of Medicine volume 6, pages 173–186 (2012)Cite this article

Abstract

Infectious diseases, mostly caused by bacteria and viruses but also a result of fungal and parasitic infection, have been one of the most important public health concerns throughout human history. The first step in combating these pathogens is to get a timely and accurate diagnosis at an affordable cost. Many kinds of diagnostics have been developed, such as pathogen culture, biochemical tests and serological tests, to help detect and fight against the causative agents of diseases. However, these diagnostic tests are generally unsatisfactory because they are not particularly sensitive and specific and are unable to deliver speedy results. Nucleic acid-based diagnostics, detecting pathogens through the identification of their genomic sequences, have shown promise to overcome the above limitations and become more widely adopted in clinical tests. Here we review some of the most popular nucleic acid-based diagnostics and focus on their adaptability and applicability to routine clinical usage. We also compare and contrast the characteristics of different types of nucleic acid-based diagnostics.

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Affiliations

  1. Neuroscience Research Institute; Department of Neurobiology, School of Basic Medical Sciences; Key Laboratory for Neuroscience, Ministry of Education; Key Laboratory for Neuroscience, Ministry of Health, Peking University, Beijing, 100191, China

    Albert Cheung-Hoi Yu

  2. Hai Kang Life Corporation Limited, Hong Kong SAR, China, Beijing, 100176, China

    Albert Cheung-Hoi Yu, Greg Vatcher, Xin Yue, Yan Dong, Mao Hua Li, Patrick H. K. Tam, Parker Y. L. Tsang, April K. Y. Wong, Michael H. K. Hui, Bin Yang, Hao Tang & Lok-Ting Lau

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  1. Albert Cheung-Hoi Yu
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Correspondence to Albert Cheung-Hoi Yu or Lok-Ting Lau.

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Yu, A.CH., Vatcher, G., Yue, X. et al. Nucleic acid-based diagnostics for infectious diseases in public health affairs. Front. Med. 6, 173–186 (2012). https://doi.org/10.1007/s11684-012-0195-5

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  • DOI: https://doi.org/10.1007/s11684-012-0195-5

Keywords

  • nucleic acid-based diagnostics
  • infectious disease
  • PCR
  • NASBA
  • LAMP
  • microarray
  • LOAC
  • public health affairs