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Abnormal expression of c-Myc in human bronchial epithelial cells malignantly transformed by anti-BPDE

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Abstract

Anti-benzo[a]pyrene-7,8-diol-9,10-epoxide (anti-BPDE) is a metabolite of benzo[a]pyrene (B[a]P) and acts as a potent mutagen in mammalian systems. However, molecular mechanisms related to anti-BPDE-induced carcinogenesis are poorly understood. Here, we investigated the expression of proto-oncogene c-myc in human bronchial epithelial cells (16HBE-T) transformed by exposure to anti-BPDE. The levels of mRNA and protein of c-Myc were examined in the 16HBE-T and vehicletreated control cells (16HBE-N) by using different methods respectively, including reverse transcriptase-polymerase chain reaction (RT-PCR), quantitative real-time PCR (Q-PCR), western blot and immunocytochemical methods. The level of c-myc mRNA appeared to be significantly increased in 16HBE-T, as compared with those of the 16HBE-N. Likewise, the expression of c-Myc protein was significantly enhanced as compared with those of the control cells. Moreover, the localization of c-Myc protein shows mainly nuclear staining in 16HBE-T. In conclusion, the abnormal expression of c-Myc was present in anti-BPDE malignantly transformed 16HBE cells, which may be involved in the carcinogenesis molecular mechanism of anti-BPDE.

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Authors and Affiliations

  1. Department of Occupational and Environmental Health, the Ministry of Education Key Laboratory of Environment and Health, School of Public Health, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, 430030, China

    Juan Fu & Xuemin Chen

  2. Institute for Chemical Carcinogenesis, Guangzhou Medical College, Guangzhou, 510182, China

    Juan Fu & Yiguo Jiang

Authors
  1. Juan Fu
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  2. Yiguo Jiang
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  3. Xuemin Chen
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Correspondence to Yiguo Jiang or Xuemin Chen.

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Fu, J., Jiang, Y. & Chen, X. Abnormal expression of c-Myc in human bronchial epithelial cells malignantly transformed by anti-BPDE. Front. Med. China 2, 380–385 (2008). https://doi.org/10.1007/s11684-008-0073-3

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  • DOI: https://doi.org/10.1007/s11684-008-0073-3

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