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Influence and related mechanism of Retn gene expression on glucose uptake in 3T3-L1 cells

Frontiers of Medicine in China volume 1, pages 269–273 (2007)Cite this article

Abstract

The aim of this article was to investigate the influence and the related mechanism of the Retn gene on glucose uptake and insulin resistance in 3T3-L1 cells. Radioimmuno-assay was used to determine glucose uptake in 3T3-L1 cells with different Retn gene expression levels, whether cells were stimulated by insulin or not. RT-PCR and real-time RT-PCR analysis were used to determine the mRNA levels of several glucose transport proteins in 3T3-L1 cells with different Retn gene expression levels, including insulin receptor substrate-1(IRS-1), phosphatidylinositol 3-kinase (PI-3K), AKT-2, glucose transporter-4 (GLUT-4), p38 mitogen-activated protein kinase (p38MAPK) and glycogen synthase kinase-3β (GSK-3β). The glucose uptake decreased with the increase in Retn gene expression in 3T3-L1 cells, which was independent of whether the cells were stimulated by insulin or not. The mRNA expression of two signal proteins PI-3K and AKT-2 decreased and the other two signal proteins, GSK-3β and p38MAPK, increased with Retn overexpression in 3T3-L1 cells. Resistin could induce insulin resistance in adipocytes, which might be related to the changes of some proteins in PI-3K and Ras pathways.

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Affiliations

  1. Institute for Nutritional Science, Shanghai Institute for Biological Sciences, Chinese Academy of Science, Shanghai, 200031, China

    Li Huaixing MD

  2. Center for Healthy Food Evaluation, State Food and Drug Administration, Beijing, 100061, China

    Li Yahui MD, Dong Shiyuan BS, Yu Chao MS & Jiang Yu MS

  3. Department of Medical Genetics, College of Basic Medical Science, Second Military Medical University, Shanghai, 200433, China

    Sun Shuhan MD

Authors
  1. Li Yahui MD
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  2. Li Huaixing MD
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  3. Dong Shiyuan BS
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  4. Yu Chao MS
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  5. Jiang Yu MS
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  6. Sun Shuhan MD
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Corresponding author

Correspondence to Li Yahui MD.

Additional information

Translated from Academic Journal of Second Military Medical University, 2006, 27(10): 1,067–1,071 [译自: 第二军医大学学报]

The authors contributed equally to the study.

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Li, Y., Li, H., Dong, S. et al. Influence and related mechanism of Retn gene expression on glucose uptake in 3T3-L1 cells. Front. Med. China 1, 269–273 (2007). https://doi.org/10.1007/s11684-007-0051-1

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  • DOI: https://doi.org/10.1007/s11684-007-0051-1

Keywords

  • Resistin
  • Retn gene
  • insulin
  • glucose uptake
  • 3T3-L1 cells