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Effect of intestinal ischemia/reperfusion injury on leptin and orexin-A levels

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Abstract

The aim of this paper is to explore the effect of intestinal ischemia/reperfusion (I/R) injury on leptin and orexin-A levels in peripheral blood and central secretory tissues, and to examine the roles of leptin and orexin-A in acute inflammatory responses. An intestinal I/R injury model of rats was made; the rats were grouped according to the time of after 60 min ischemia. Radioimmunoassay was employed to detect the levels of leptin in serum and adipose tissue and orexin-A levels in plasma and hypothalamus. Reverse transcriptase-polymerase chain reaction was used to detect mRNA expressions of adipose leptin and hypothalamus orexin-A. Compared with the levels before the injury, serum leptin in 60 min ischemia/30 min reperfusion (I60′R30′) group decreased and that of I60′R360′ group increased. Compared with sham-operation group (sham group) after injury, serum leptin level of I60′R360′ group increased, adipose leptin levels of I60′R30′ and I60′R90′ decreased, and adipose leptin in I60′R360′ group increased. After the injury, adipose leptin mRNA expressions of I60′R30′, I60′R240′ and I60′R360′ increased, whereas that of I60′R150′ group decreased as compared with the sham group. There was no significant difference in the protein levels of orexin-A, either between plasma and hypothalamus or between pre-and post-I/R injury. Compared with sham group, hypothalamus orexin-A mRNA expressions of I60′R30′ and I60′R90′ decreased gradually after the injury, with that of I60′R150′ group reaching the lowest, and those of I60′R240′ and I60′R360′ recovering gradually, although they were still significantly lower than that of sham group. Leptin and orexin-A respond to intestinal I/R injury in a time-dependent manner, with leptin responding more quickly than orexin-A does, and both of them may contribute to the metabolic disorders in acute inflammation.

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Authors and Affiliations

  1. Research Laboratory of Biochemistry, General Hospital of PLA, Beijing, 100853, China

    Lin Ji MD, Yan Guangtao MD, Hao Xiuhua BS & Zhang Kai BS

  2. Department of Pediatrics, General Hospital of PLA, Beijing, 100853, China

    Gao Xiaoning MD

  3. Medical and Experimental Analysis Center, General Hospital of PLA, Beijing, 100853, China

    Liao Jie MD

Authors
  1. Lin Ji MD
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  2. Yan Guangtao MD
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  3. Gao Xiaoning MD
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  4. Liao Jie MD
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  5. Hao Xiuhua BS
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  6. Zhang Kai BS
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Corresponding author

Correspondence to Yan Guangtao MD.

Additional information

Translated from Journal of Southern Medical University, 2006, 26(6): 719–724 [译自: 南方医科大学学报]

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Lin, J., Yan, G., Gao, X. et al. Effect of intestinal ischemia/reperfusion injury on leptin and orexin-A levels. Front. Med. China 1, 87–92 (2007). https://doi.org/10.1007/s11684-007-0017-3

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  • DOI: https://doi.org/10.1007/s11684-007-0017-3

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