Skip to main content

The lectin gene TRpL1 of tetraploid Robinia pseudoacacia L. response to salt stress

Abstract

Lectins are natural proteins in animals, plants, and microorganisms and can be divided into 12 families. These lectins play important roles in various environmental stresses. Some polyploid plants show tolerance to environmental stresses and to insect pests. However, the mechanism of stress tolerance is unclear. Tetraploid Robinia pseudoacacia (4×) under salt stress showed higher tolerance than diploid R. pseudoacacia (2×). As lectin can improve stress tolerance, it was questioned whether the stress resistance of polyploid plants was related to the lectin protein. In this study, salt resistance of lectin gene TRpL1 was verified by its over-expression in plants. In addition, salt resistance of lectin protein by E. coli strains was detected. The data revealed that the over-expression transgenic plants of TRpL1 showed better salt tolerance than control plants under salt stress, and the TRpL1-expressing strain also grew better in the medium with added NaCl. Therefore, tetraploid plants can resist salt stress through TRpL1 protein regulation.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

References

  • Abdel Latef AAH, Mostofa MG, Rahman MM, Abdel-Farid IB, Lam-Son Phan T (2019) Extracts from yeast and carrot roots enhance maize performance under seawater-induced salt stress by altering physio-biochemical characteristics of stressed plants. J Plant Growth Regul 38:966–979

    CAS  Article  Google Scholar 

  • Babosha AV (2008) Inducible lectins and plant resistance to pathogens and abiotic stress. Biochemistry-Moscow 73:812–825

    CAS  Article  Google Scholar 

  • Bezrukova M, Kildibekova A, Shakirova F (2008) WGA reduces the level of oxidative stress in wheat seedlings under salinity. Plant Growth Regul 54:195–201

    CAS  Article  Google Scholar 

  • Cao Y, Jiang MQ, Xu FL, Liu S, Meng FJ (2017) The effects of elevated CO2 (0.5%) on chloroplasts in the tetraploid black locust (Robinia pseudoacacia L.). Ecol Evol 7:10546–10555

    Article  Google Scholar 

  • Comai L (2005) The advantages and disadvantages of being polyploid. Nat Rev Genet 6:836–846

    CAS  Article  Google Scholar 

  • Deng Y, Srivastava R, Howell SH (2013) Protein kinase and ribonuclease domains of IRE1 confer stress tolerance, vegetative growth, and reproductive development in Arabidopsis. Proc Natl Acad Sci USA 110:19633–19638

    CAS  Article  Google Scholar 

  • Gong ZZ, Xiong LM, Shi HZ, Yang SH, Herrera-Estrella LR, Xu GH, Chao DY, Li JR, Wang PY, Qin F, Li JJ, Ding YL, Shi YT, Wang Y, Yang YQ, Guo Y, Zhu JK (2020) Plant abiotic stress response and nutrient use efficiency. Sci China Life Sci 63:635–674

    Article  Google Scholar 

  • Jiang SY, Ma Z, Ramachandran S (2010) Evolutionary history and stress regulation of the lectin superfamily in higher plants. BMC Evol Biol 10:79

    Article  Google Scholar 

  • Kil’dibekova AR, Bezrukova MV, Aval’baev AM, Fatkhutdinova RA, Shakirova FM (2004) Mechanisms of protective action of wheat germ agglutinin on cell growth in wheat seedling roots under salinity. Tsitologiia 46:312–316

    CAS  PubMed  Google Scholar 

  • Komarova EN, Trunova TI, Vyskrebentseva EI (2000) Lectin activity changes in individual subcellular fractions of crown meristem of winter wheat during the first day of cold adaptation. Doklady biochemistry: proceedings of the Academy of Sciences of the USSR, Biochemistry section 373

  • Lannoo N, Van Damme EJM (2014) Lectin domains at the frontiers of plant defense. Front Plant Sci 5:397

    PubMed  PubMed Central  Google Scholar 

  • Luo QX, Peng M, Zhang XL, Lei P, Ji XM, Chow W, Meng FJ, Sun GY (2017) Comparative mitochondrial proteomic, physiological, biochemical and ultrastructural profiling reveal factors underpinning salt tolerance in tetraploid black locust (Robinia pseudoacacia L.). BMC Genomics 18:648

    Article  Google Scholar 

  • Meng FJ, Peng M, Pang HY, Huang FL (2014) Comparison of photosynthesis and leaf ultrastructure on two black locust (Robinia pseudoacacia L.). Biochem Syst Ecol 55:170–175

    CAS  Article  Google Scholar 

  • Meng FJ, Luo QX, Wang QY, Zhang XL, Qi ZH, Xu FL, Lei X, Cao Y, Chow WS, Sun GY (2016) Physiological and proteomic responses to salt stress in chloroplasts of diploid and tetraploid black locust (Robinia pseudoacacia L.). Sci Rep 6:23098

    CAS  Article  Google Scholar 

  • Morton MJL, Awlia M, Al-Tamimi N, Saade S, Pailles Y, Negrao S, Tester M (2019) Salt stress under the scalpel—dissecting the genetics of salt tolerance. Plant J 97:148–163

    CAS  Article  Google Scholar 

  • Ors S, Suarez DL (2017) Spinach biomass yield and physiological response to interactive salinity and water stress. Agric Water Manag 190:31–41

    Article  Google Scholar 

  • Podda A, Checcucci G, Mouhaya W, Centeno D, Rofidal V, Del Carratore R, Luro F, Morillon R, Ollitrault P, Maserti BE (2013) Salt-stress induced changes in the leaf proteome of diploid and tetraploid mandarins with contrasting Na+ and Cl accumulation behaviour. J Plant Physiol 170:1101–1112

    CAS  Article  Google Scholar 

  • Rudiger H (1998) Plant lectins—more than just tools for glycoscientists: occurrence, structure, and possible functions of plant lectins. Acta Anat 161:130–152

    CAS  Article  Google Scholar 

  • Ruiz M, Oustric J, Santini J, Morillon R (2020) Synthetic polyploidy in grafted crops. Front Plant Sci 11:540894

    Article  Google Scholar 

  • Sadeghi A, Smagghe G, Broeders S, Hernalsteens JP, De Greve H, Peumans WJ, Van Damme EJM (2008) Ectopically expressed leaf and bulb lectins from garlic (Allium sativum L.) protect transgenic tobacco plants against cotton leafworm (Spodoptera littoralis). Transgenic Res 17:9–18

    CAS  Article  Google Scholar 

  • Sharon N, Lis H (1989) Lectins as cell recognition molecules. Science (New York, N.Y.) 246:227–234

    CAS  Article  Google Scholar 

  • Spadoro-Tank JP, Etzler ME (1988) Heath shock enhances the synthesis of a lectin-related protein in dolichos biflorus cell suspension cultures. Plant Physiol 88:1131–1135

    CAS  Article  Google Scholar 

  • Tsaneva M, Van Damme EJM (2020) 130 years of plant lectin research. Glycoconj J 37:533–551

    CAS  Article  Google Scholar 

  • Van de Peer Y, Ashman TL, Soltis PS, Soltis DE (2021) Polyploidy: an evolutionary and ecological force in stressful times. Plant Cell 33:11–26

    Article  Google Scholar 

  • Van Holle S, Smagghe G, Van Damme EJM (2016) Overexpression of Nictaba-Like lectin genes from Glycine max confers tolerance toward pseudomonas syringae infection, aphid infestation and salt stress in transgenic Arabidopsis plants. Front Plant Sci 7:1590

    PubMed  PubMed Central  Google Scholar 

  • Xu LP, Ji XM, Jin GZ, Guan FC, Luo QX, Meng FJ (2020) Improvement of salt tolerance on Escherichia coli by expression of agglutinin from Amygdalus mira. Int J Agric Biol 23:333–339

    CAS  Google Scholar 

  • Zhang WW, Song J, Wang M, Liu YY, Li N, Zhang YJ, Holbrook NM, Hao GY (2017) Divergences in hydraulic architecture form an important basis for niche differentiation between diploid and polyploid species in NE China. Tree Physiol 37:604–616

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Contributions

SL and YJ contributed equally to this work. LX, SL, XG and YJ set up the experiment. SL wrote the manuscript. LX, PL, JL and FM edited the manuscript. JL, QL, WL and LT critically revised the draft and updated the manuscript for publication.

Corresponding authors

Correspondence to Lei Tao or Fanjuan Meng.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Project funding: The work was supported by the National Natural Science Foundation of China (32071728).

The online version is available at http://www.springerlink.com.

Corresponding editor: Tao Xu.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Liu, S., Jiang, Y., Guo, X. et al. The lectin gene TRpL1 of tetraploid Robinia pseudoacacia L. response to salt stress. J. For. Res. (2022). https://doi.org/10.1007/s11676-022-01479-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s11676-022-01479-0

Keywords

  • Tetraploid Robinia pseudoacacia lectin
  • Salt stress
  • Polyploid