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Matrine inhibits mycelia growth of Botryosphaeria dothidea by affecting membrane permeability

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Abstract

Matrine is a promising botanical antifungal; however, the mechanism underlying the antifungal activity is yet limited. We studied the antifungal activity of matrine and the underlying mechanism in Botryosphaeria dothidea as a model strain. Matrine strongly inhibited mycelial growth of B. dothidea in a dose-dependent manner. Matrine-treated B. dothidea showed morphological and ultrastructural alterations, including shriveled hyphae, plasmolysis, and leakage of cytoplasm related to cell membrane deterioration. In addition, matrine caused significantly high conductivity and absorbance (260 nm) in extracellular matrices and low lipid contents in B. dothidea, indicating increased membrane permeability. Lipid peroxidation showed that matrine resulted in increased malondialdehyde content while enhancing the generation of reactive oxygen species and the activities of superoxide dismutase, catalase, and peroxidase. These results showed that matrine inhibited the mycelial growth of B. dothidea by enhancing cell membrane permeability via membrane lipid peroxidation.

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Acknowledgements

I am grateful to all my colleagues that assisted in writing this thesis, particularly my supervisor, Dr. Ling Ma, who offered me valuable suggestions in my academic study.

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Authors and Affiliations

  1. College of Forestry, Northeast Forestry University, Harbin, 150040, People’s Republic of China

    Jialiang Pan, Xin Hao, Hanwen Yao, Kangkang Ge & Ling Ma

  2. General Station of Forest and Grassland Pest Management, National Forestry and Grassland Administration, Shenyang, 110034, People’s Republic of China

    Jialiang Pan & Hanwen Yao

  3. College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin, 150040, People’s Republic of China

    Wei Ma

Authors
  1. Jialiang Pan
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  2. Xin Hao
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  3. Hanwen Yao
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  4. Kangkang Ge
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  5. Ling Ma
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  6. Wei Ma
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Contributions

Jialiang Pan and Xin Hao are equal Contribution to this paper.

Corresponding authors

Correspondence to Ling Ma or Wei Ma.

Additional information

Project funding: The project was fully funded by the Special Fund for Forest-Scientific Research in the Public Interest (No. 201304403-4) and the Fundamental Research Funds for the Central Universities (2572015AA25).

The online version is available at http://www.springerlink.com

Corresponding editor: Zhu Hong.

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Pan, J., Hao, X., Yao, H. et al. Matrine inhibits mycelia growth of Botryosphaeria dothidea by affecting membrane permeability. J. For. Res. 30, 1105–1113 (2019). https://doi.org/10.1007/s11676-019-00883-3

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  • DOI: https://doi.org/10.1007/s11676-019-00883-3

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