Extraction of seed oil from Diospyros lotus optimized using response surface methodology

  • Gholamhossein Sodeifian
  • Nedasadat Saadati Ardestani
  • Seyed Ali Sajadian
Original Paper


Oil from seeds of Diospyros lotus was extracted using a conventional method with two different solvents: hexane and petroleum ether. A central composite design with response surface methodology were used to optimize the process. A second-order polynomial equation was employed, and ANOVA was applied to evaluate the impact of various operating parameters including extraction temperature (x1; 44.9–70.1 °C), extraction time (x2; 5.0–10.0 h) and solvent to solid ratio (x3; 11.6–28.4 mL g−1), on oil yield. Experiments to validate the model showed decent conformity between predicted and actual values. Extraction conditions for optimal oil yield were 61 °C, 8.75 h extraction duration and 19.25 mL g−1 solvent to solid ratio. Under these conditions, the oil yield was predicted to be 5.1340%. Oil samples obtained were then analyzed using gas chromatography. The fatty acid composition revealed the major fatty acids to be oleic acid (C18:1) and linoleic acid (C18:2). The analysis of oil also demonstrated a decent ratio between omega-3 and omega-6 fatty acids. The structure of seeds was imaged using scanning electron microscopy. Oil quality was analyzed thermogravimetrically and by Fourier transform infrared spectroscopy. The assigned nutritional features of the D. lotus oil suggested that it can be used as an edible oil in pharmaceutical and food industry in the future.


Diospyros lotus Solvent extraction Physicochemical properties Fatty acids Response surface methodology 



The authors gratefully acknowledge the University of Kashan, especially the Deputy of Research, for financial support (Grant: Pajoohaneh #1394/12). They also thank the Laboratory of Supercritical Fluids at the University of Kashan for providing experimental facilities and the laboratory staff at the Nargesoil Company (Shiraz, Iran) for cooperation in this project.


  1. Akintunde AM, Ajala SO, Betiku E (2015) Optimization of Bauhinia monandra seed oil extraction via artificial neural network and response surface methodology: a potential biofuel candidate. Ind Crops Prod 67:387–394CrossRefGoogle Scholar
  2. Azmir J, Zaidul I, Rahman M, Sharif K, Sahena F, Jahurul M, Mohamed A (2014) Optimization of oil yield of Phaleria macrocarpa seed using response surface methodology and its fatty acids constituents. Ind Crops Prod 52:405–412CrossRefGoogle Scholar
  3. Bibi N, Khattak AB, Mehmood Z (2007) Quality improvement and shelf life extension of persimmon fruit (Diospyros kaki). J Food Eng 79(4):1359–1363CrossRefGoogle Scholar
  4. Canfield LM, Krinsky NI, Olson JA (1993) Carotenoids in human health. Annals of the New York Academy of Sciences, New York, pp 130–178Google Scholar
  5. Dias ALB, Sergio CSA, Santos P, Barbero GF, Rezende CA, Martínez J (2016) Effect of ultrasound on the supercritical CO2 extraction of bioactive compounds from dedo de moça pepper (Capsicum baccatum L. var. pendulum). Ultrason Sonochem 31:284–294CrossRefPubMedGoogle Scholar
  6. Duan J, Zheng Y, Dong Q, Fang J (2004) Structural analysis of a pectic polysaccharide from the leaves of Diospyros kaki. Phytochemistry 65(5):609–615CrossRefPubMedGoogle Scholar
  7. Duan L, Jiang R, Shi Y, Duan C, Wu G (2016) Optimization of ultrasonic-assisted extraction of higher fatty acids in grape berries (seed-free fruit sections). Anal Methods 8(32):6208–6215CrossRefGoogle Scholar
  8. Dubois V, Breton S, Linder M, Fanni J, Parmentier M (2007) Fatty acid profiles of 80 vegetable oils with regard to their nutritional potential. Eur J Lipid Sci Technol 109(7):710–732CrossRefGoogle Scholar
  9. Farhoosh R, Einafshar S, Sharayei P (2009) The effect of commercial refining steps on the rancidity measures of soybean and canola oils. Food Chem 115(3):933–938CrossRefGoogle Scholar
  10. Fasina O, Craig-Schmidt M, Colley Z, Hallman H (2008) Predicting melting characteristics of vegetable oils from fatty acid composition. LWT Food Sci Technol 41(8):1501–1505CrossRefGoogle Scholar
  11. Hajra B, Sultana N, Pathak AK, Guria C (2015) Response surface method and genetic algorithm assisted optimal synthesis of biodiesel from high free fatty acid sal oil (Shorea robusta) using ion-exchange resin at high temperature. J Environ Chem Eng 3(4):2378–2392CrossRefGoogle Scholar
  12. Hao JY, Han W, Xue BY, Deng X (2002) Microwave-assisted extraction of artemisinin from Artemisia annua L. Sep Purif Technol 28(3):191–196CrossRefGoogle Scholar
  13. Jain S, Sharma M (2012) Application of thermogravimetric analysis for thermal stability of Jatropha curcas biodiesel. Fuel 93:252–257CrossRefGoogle Scholar
  14. Jang I-C, Jo E-K, Bae S-M, Bae M-S, Lee H-J, Park E, Yuk H-G, Ahn G-H, Lee S-C (2010) Antioxidant activity and fatty acid composition of four different persimmon seeds. Food Sci Technol Res 16(6):577–584CrossRefGoogle Scholar
  15. Jeong EY, Jeon JH, Lee CH, Lee H-S (2009) Antimicrobial activity of catechol isolated from Diospyros kaki Thunb. roots and its derivatives toward intestinal bacteria. Food Chem 115(3):1006–1010CrossRefGoogle Scholar
  16. Kostić MD, Joković NM, Stamenković OS, Rajković KM, Milić PS, Veljković VB (2013) Optimization of hempseed oil extraction by n-hexane. Ind Crops Prod 48:133–143CrossRefGoogle Scholar
  17. Kwon J-H, Belanger JM, Pare JJ, Yaylayan VA (2003) Application of the microwave-assisted process (MAP™) to the fast extraction of ginseng saponins. Food Res Int 36(5):491–498CrossRefGoogle Scholar
  18. Lee YM, Kim CC (1994) Studies on the fatty acid composition of sweet persimmons (Diospyros kaki L.). J Korean Soc Hortic Sci 35:233–240Google Scholar
  19. Liu HC, Li P, Wang G, Yu HP, Zeng ZQ, Yang D (2012) Optimization for extraction of astaxanthin from shrimp shell using response surface method. Adv Mater Res 396:609–613Google Scholar
  20. Lu X, Rasco BA (2012) Determination of antioxidant content and antioxidant activity in foods using infrared spectroscopy and chemometrics: a review. Crit Rev Food Sci Nutr 52(10):853–875CrossRefPubMedGoogle Scholar
  21. Mallavadhani U, Panda AK, Rao Y (1998) Review article number 134 pharmacology and chemotaxonomy of diospyros. Phytochemistry 49(4):901–951CrossRefPubMedGoogle Scholar
  22. Ong AS, Tee E (1992) [14] Natural sources of carotenoids from plants and oils. Methods Enzymol 213:142–167CrossRefGoogle Scholar
  23. Orhan I, Sener B (2002) Fatty acid content of selected seed oils. J Herb Pharm 2(3):29–33CrossRefGoogle Scholar
  24. Rai A, Mohanty B, Bhargava R (2015) Modeling and response surface analysis of supercritical extraction of watermelon seed oil using carbon dioxide. Sep Purif Technol 141:354–365CrossRefGoogle Scholar
  25. Rai A, Mohanty B, Bhargava R (2016) Supercritical extraction of sunflower oil: a central composite design for extraction variables. Food Chem 192:647–659CrossRefPubMedGoogle Scholar
  26. Rout J, Tripathy S, Misra M, Mohanty A (2001) Scanning electron microscopy study of chemically modified coir fibers. J Appl Polym Sci 79(7):1169–1177CrossRefGoogle Scholar
  27. Shim JH, Abd El-Aty A, Choi JH, Kang CA (2007) Determination of field-incurred pyrimethanil residues in persimmon (Diospyros kaki Linn) by liquid chromatography. Biomed Chromatogr 21(12):1279–1283CrossRefPubMedGoogle Scholar
  28. Silverstein RM, Webster FX, Kiemle DJ, Bryce DL (2014) Spectrometric identification of organic compounds. Wiley, New York, pp 94–120Google Scholar
  29. Sodeifian G, Ardestani NS, Sajadian SA, Ghorbandoost S (2016a) Application of supercritical carbon dioxide to extract essential oil from Cleome coluteoides Boiss: experimental, response surface and grey wolf optimization methodology. J Supercrit Fluids 114:55–63CrossRefGoogle Scholar
  30. Sodeifian G, Ghorbandoost S, Sajadian SA, Ardestani NS (2016b) Extraction of oil from Pistacia khinjuk using supercritical carbon dioxide: experimental and modeling. J Supercrit Fluids 110:265–274CrossRefGoogle Scholar
  31. Sodeifian G, Sajadian SA, Ardestani NS (2016c) Evaluation of the response surface and hybrid artificial neural network-genetic algorithm methodologies to determine extraction yield of Ferulago angulata through supercritical fluid. J Taiwan Inst Chem Eng 60:165–173CrossRefGoogle Scholar
  32. Sodeifian G, Sajadian SA, Ardestani NS (2016d) Extraction of Dracocephalum kotschyi Boiss using supercritical carbon dioxide: experimental and optimization. J Supercrit Fluids 107:137–144CrossRefGoogle Scholar
  33. Sodeifian G, Sajadian SA, Ardestani NS (2017) Supercritical fluid extraction of omega-3 from Dracocephalum kotschyi seed oil: process optimization and oil properties. J Supercrit Fluids 119:139–149CrossRefGoogle Scholar
  34. Subroto E, Manurung R, Heeres HJ, Broekhuis AA (2015) Optimization of mechanical oil extraction from Jatropha curcas L. kernel using response surface method. Ind Crops Prod 63:294–302CrossRefGoogle Scholar
  35. Tangmouo J, Lontsi D, Ngounou F, Kuete V, Meli A, Manfouo R, Kamdem H, Tane P, Beng VP, Sondengam B (2005) Diospyrone, a new coumarinylbinaphthoquinone from Diospyros canaliculata (Ebenaceae): structure and antimicrobial activity. Bull Chem Soc Ethiop 19(1):81–88Google Scholar
  36. Tangmouo JG, Meli AL, Komguem J, Kuete V, Ngounou FN, Lontsi D, Beng VP, Choudhary MI, Sondengam BL (2006) Crassiflorone, a new naphthoquinone from Diospyros crassiflora (Hien). Tetrahedron Lett 47(18):3067–3070CrossRefGoogle Scholar
  37. Tian Y, Xu Z, Zheng B, Lo YM (2013) Optimization of ultrasonic-assisted extraction of pomegranate (Punica granatum L.) seed oil. Ultrason Sonochem 20(1):202–208CrossRefPubMedGoogle Scholar
  38. Veberic R, Jurhar J, Mikulic-Petkovsek M, Stampar F, Schmitzer V (2010) Comparative study of primary and secondary metabolites in 11 cultivars of persimmon fruit (Diospyros kaki L.). Food Chem 119(2):477–483CrossRefGoogle Scholar
  39. Wang Y, Hossain D, Perry PL, Adams B, Lin J (2012) Characterization of volatile and aroma—impact compounds in persimmon (Diospyros kaki L., var. Triumph) fruit by GC—MS and GC—O analyses. Flavour Fragr J 27(2):141–148CrossRefGoogle Scholar
  40. Zhang C, Mu T (2011) Optimisation of pectin extraction from sweet potato (Ipomoea batatas, Convolvulaceae) residues with disodium phosphate solution by response surface method. Int J Food Sci Technol 46(11):2274–2280CrossRefGoogle Scholar

Copyright information

© Northeast Forestry University and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Gholamhossein Sodeifian
    • 1
    • 2
  • Nedasadat Saadati Ardestani
    • 1
    • 2
  • Seyed Ali Sajadian
    • 1
    • 2
  1. 1.Department of Chemical Engineering, Faculty of EngineeringUniversity of KashanKashanIran
  2. 2.Laboratory of Supercritical Fluids and NanotechnologyUniversity of KashanKashanIran

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