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Effects of a nitric oxide donor and nitric oxide scavengers on Sorbus pohuashanensis embryo germination

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Abstract

The effects of an exogenous nitric oxide donor (sodium nitroprusside, SNP), a NO scavenger 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxode (PTIO) and carboxy-PTIO potassium salt (cPTIO) on the embryo germination of Sorbus pohuashanensis were studied in a petri dish test. SNP at 0.5–5 mmol L−1 increased germination percentage, mean time to germination, germination index and germination energy compared with the control to different degrees. Treatment with 2 mmol L−1 SNP improved germination most significantly; embryo germination percentage for mother tree 1 (91.11%) and mother tree 2 (64.44%) were much higher than the control. In addition, excessive SNP levels did not enhance embryo germination. Combined treatment with SNP and an NO scavenger delayed embryo germination. Treatment with cPTIO inhibited embryo germination; germination percentage was 42.22% and was lower than that of the control. These results show that low concentrations of exogenous NO can enhance the embryo germination of S. pohuashanensis, providing a simple, effective way for promoting germination of S. pohuashanensis.

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References

  • Cao BJ, Reith MEA (2002) Nitric oxide scavenger carboxy-PTIO potentiates the inhibition of dopamine uptake by nitric oxide donors. Eur J Pharmacol 448(1):27–30

    Article  CAS  PubMed  Google Scholar 

  • Chang QS, Lx Zhang, Yang W, Ss Zhou, Huang QZ, Lü FJ, Huang Y, Ge SH, Zhang TM (2016) Effects of exogenous nitric oxide on antioxidant activity and photosynthetic characteristics of Prunella vulgaris seedlings under NaCl stress. Acta Pratacult Sinica 25(7):121–130

    Google Scholar 

  • Deng W, Qing XG, Yang Y (2010) Effects of applying organic fertilizer on rice lodging resistance and yield. J Agric Sci Technol 11(2):98–101

    Google Scholar 

  • Gniazdowska A, Dobrzyńska U, Babańczyk T, Bogatek R (2007) Breaking the apple embryo dormancy by nitric oxide involves the stimulation of ethylene production. Planta 225(4):1051–1057

    Article  CAS  PubMed  Google Scholar 

  • Gniazdowska A, Krasuska U, Czajkowska K, Bogatek R (2010) Nitric oxide, hydrogen cyanide and ethylene are required in the control of germination and undisturbed development of young apple seedlings. Plant Growth Regul 61(1):75–84

    Article  CAS  Google Scholar 

  • Jia HF, Zhang HY (2014) Effects of exogenous nitric oxide on seed germination and seedling physiological characteristics of Isatis indigotica under NaCl stress. Chin Tradit Herb Drugs 45(1):118–124

    CAS  Google Scholar 

  • Krasuska U, Ciacka K, Bogatek R, Gniazdowska A (2014) Polyamines and nitric oxide link in regulation of dormancy removal and germination of apple (Malus domestica, Borkh.) embryos. J Plant Growth Regul 33(3):590–601

    Article  CAS  Google Scholar 

  • Lamotte O, Courtois C, Barnavon L, Pugin A, Wendehenne D (2005) Nitric oxide in plants: the biosynthesis and cell signalling properties of a fascinating molecule. Planta 221(1):1–4

    Article  CAS  PubMed  Google Scholar 

  • Libourel IGL, Bethke PC, Michele RD, Jones RL (2006) Nitric oxide gas stimulates germination of dormant Arabidopsis seeds: use of a flow-through apparatus for delivery of nitric oxide. Planta 223(4):813–820

    Article  CAS  PubMed  Google Scholar 

  • Ma Z, Marsolais F, Bykova NV, Igamberdiev AU (2016) Nitric oxide and reactive oxygen species mediate metabolic changes in barley seed embryo during Germination. Front Plant Sci 7(126):1–13

    PubMed Central  PubMed  Google Scholar 

  • Petruzzelli L, Coraggio I, Leubner-Metzger G (2000) Ethylene promotes ethylene biosynthesis during pea seed germination by positive feedback regulation of 1-aminocyclo-propane-1-carboxylic acid oxidase. Planta 211(1):144–149

    Article  CAS  PubMed  Google Scholar 

  • Sanz L, Albertos P, Mateos I, Sánchez-Vicente I, Lechón T, Fernández-Marcos M, Lorenzo O (2015) Nitric oxide (NO) and phytohormones crosstalk during early plant development. J Exp Bot 66(10):2857–2868

    Article  CAS  PubMed  Google Scholar 

  • Shen H (2006) Influencing factors to seed dormancy and germination characteristics of Sorbus pohuashanensis. Scientia Silvae Sinicae 42(10):133–138

    Google Scholar 

  • Tang WQ, Zhang YX, Liu BH (2014) Grafting survival rate in different strains of Sorbus spp. Prot For Sci Technol 7:14–15

    Google Scholar 

  • Wang P, Zhu JK, Lang Z (2015) Nitric oxide suppresses the inhibitory effect of abscisic acid on seed germination by S-nitrosylation of SnRK2 proteins. Plant Signal Behav 10(6):1–3

    Google Scholar 

  • Xu JW, Shen HL, Zhang XL, Zhang P, Huang J (2010) Sorbus pohuashanensis seed dispersal and germination and their relationships with population natural regeneration. Chin J Appl Ecol 21(10):2536–2544

    Google Scholar 

  • Yang L (2013) Effects of exogenous nitric oxide on embryo germination and ROS accumulation in seedling growth initial stage of Sorbus pohuashanensis. Scientia Silvae Sinicae 49(6):60–67

    Google Scholar 

  • Yang L, Shen HL (2011) Effect of electrostatic field on seed germination and seedling growth of Sorbus pohuashanesis. J For Res 22(1):27–34

    Article  CAS  Google Scholar 

  • Yang L, Zhang P (2011) Experimental course of tree seeds. Northeast Forestry University Press, Harbin, pp 85–87

    Google Scholar 

  • Yang L, Liu CP, Shen HL (2008) Effect of cold stratification durations and germination temperatures on seed germination of Sorbus Pohuashanensis. Seed 27(10):20–22

    Google Scholar 

  • Yang L, Shen HL, Liu CP, Zhai XJ (2010) Somatic embryogenesis from mature zygotic embryo explants of Sorbus pohuashanensis Hedl. Bull Bot Res 30(2):174–179

    CAS  Google Scholar 

  • Yin H, Liu Q (2004) Advances in studies on molecular biology of seed dormancy and germination. Chin Bull Bot 21(2):156–163

    Google Scholar 

  • Zhang YY (2015) Study on the Technology of Microcutting and Rooting in Sorbus aucuparia L. Planting in Harbin. Journal of Anhui. Agric Sci 43(22):128–131

    CAS  Google Scholar 

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Acknowledgements

We thank all the colleagues in our lab for constructive discussion and technical support.

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Authors and Affiliations

  1. State Key Laboratory of Forest Genetics and Breeding, School of Forestry, Northeast Forestry University, Harbin, 150040, People’s Republic of China

    Ling Yang, Dongyan Zhang, Hongnan Liu, Cheng Wei, Jianan Wang & Hailong Shen

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  1. Ling Yang
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  2. Dongyan Zhang
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  3. Hongnan Liu
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  4. Cheng Wei
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  5. Jianan Wang
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  6. Hailong Shen
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Corresponding author

Correspondence to Ling Yang.

Additional information

Project funding: This research was supported by the Science Foundation of Heilongjiang Province of China (C201407) and the National Natural Science Foundation of China (No. 31400535).

The online version is available at http://www.springerlink.com

Corresponding editor: Zhu Hong.

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Yang, L., Zhang, D., Liu, H. et al. Effects of a nitric oxide donor and nitric oxide scavengers on Sorbus pohuashanensis embryo germination. J. For. Res. 29, 631–638 (2018). https://doi.org/10.1007/s11676-017-0507-3

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  • DOI: https://doi.org/10.1007/s11676-017-0507-3

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