Abstract
Our previous studies have revealed that the ThCAP gene plays a vital role in transgenic Populus (P. davidiana × P. bolleana) in response to cold stress. However, the regulatory mechanism of ThCAP gene expression has been unclear. In this study, the 5′ flanking region of the ThCAP promoter (PThCAP) was cloned using a genome-walking method. By analyzing cis-acting regulatory elements of PThCAP, a DRE motif and MYC and MYB elements were found to be located in the promoter. To identify the regulatory elements that control the expression of the ThCAP gene promoter, a series of deletion derivatives of PThCAP, P1–P5, from the translation start code (−1538, −1190, −900, −718 and −375 bp), were fused to the GUS reporter gene, and then each deletion was stably introduced into Arabidopsis thaliana plants. Deletion analysis of the promoter suggested that only the P2 fragment had strong GUS expression in leaves and roots of A. thaliana exposed to low temperature stress. These results suggest that this 290-bp region (−1190 to −900 bp), as an important part in PThCAP, was associated with cold tolerance of A. thaliana. Our results provide evidence for the regulatory mechanism of ThCAP gene involved in the response to cold stress, and that the gene is promising candidate gene for genetic improvement of crops.
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Abbreviations
- ThCAP :
-
Tamarix hispida cold acclamation protein
- PThCAP :
-
ThCAP promoter
- COR:
-
Cold-regulated gene
- CaMV35S:
-
Cauliflower mosaic virus 35S
- UTR:
-
Untranslated region
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Project funding: This work was supported by the State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University) (201102), Natural Science Foundation of Heilongjiang Province (QC2012C057), China Postdoctoral Science Foundation (2013M531005), Foundation for the Postdoctoral of Heilongjiang Province (LBH-Z12007) and National Natural Science Foundation of China (31200510).
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Corresponding editor: Yanbo Hu.
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Guo, X., Lv, Y., Li, H. et al. A novel cold-inducible promoter, PThCAP from Tamarix hispida, confers cold tolerance in transgenic Arabidopsis thaliana . J. For. Res. 29, 331–337 (2018). https://doi.org/10.1007/s11676-017-0399-2
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DOI: https://doi.org/10.1007/s11676-017-0399-2