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Genetic diversity in two threatened species in Vietnam: Taxus chinensis and Taxus wallichiana

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Abstract

Taxus chinensis and T. wallichiana in have been threatened in their distribution areas in recent decades because of their over-exploitation and reduction and destruction of native habitats. Determining the genetic diversity in populations of the two species will provide guidelines for their protection and preservation. Two hundred and fifteen trees from six populations of T. chinensis and 150 sampled trees of T. wallichiana were sampled. Six microsatellite primer pairs selected from 16 primer pairs were used to investigate genetic variation at the population and species levels. Five yielded polymorphic alleles, and among the 13 putative alleles amplified, 11 were polymorphic (accounting for 76.33 %).Shannon’s information index (I) and percentage of polymorphic bands (PPB) (I = 0.202 and PPB = 67.22 % for T. chinensis; I = 0.217 and PPB = 65.03 % for T. wallichiana). Both species had low levels of genetic diversity (mean H o = 0.107, H e = 0.121 for T. chinensis; H o = 0.095, H e = 0.109 for T. wallichiana). Genetic differentiation among populations was higher (F ST = 0.189) for T. chinensis and lower (0.156) for T. wallichiana, indicating limited gene flow (Nm) among populations for T. chinensis (0.68) and T. wallichiana (0.65). Variation among individuals of T. chinensis was 63.59 and 73.12 % for T. wallichiana. Thus, the threatened status of the two conifers is related to a lack of genetic diversity. All populations are isolated in small forest remnants. An ex situ conservation site should be established with a new population for these species that comprises all the genetic groups for the best chance to improve their fitness under environmental stresses.

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Authors and Affiliations

  1. College of Forestry, Northwest A&F University, Yangling, 712100, Shaanxi, People’s Republic of China

    Dinh Duy Vu, Thi Tuyet Xuan Bui, Xiaohua Huang & Yi Zhang

  2. Vietnam National Museum of Nature, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam

    Dinh Duy Vu & Minh Tam Nguyen

  3. Institute of Ecology and Biological Resource (VAST), 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam

    Thi Tuyet Xuan Bui, Minh Tam Nguyen & Minh Duc Nguyen

  4. Institute of Natural Products Chemistry (VAST), 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam

    Dinh Giap Vu

  5. Institute of Forest Biotechnology, Vietnam Forestry University, Xuan Mai, Chuong My, Hanoi, Vietnam

    Van Thang Bui

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  1. Dinh Duy Vu
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  2. Thi Tuyet Xuan Bui
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  3. Minh Tam Nguyen
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  4. Dinh Giap Vu
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  5. Minh Duc Nguyen
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  6. Van Thang Bui
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  7. Xiaohua Huang
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  8. Yi Zhang
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Corresponding author

Correspondence to Xiaohua Huang.

Additional information

Project funding: This work was supported by a research project (No. Z111021402) of Northwest A&F University and by the Ministry of Natural Resources and Environment, Vietnam government, Hanoiand IDEA WILD equipment to Vu Dinh Duy, Bui Thi Tuyet Xuan.

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Corresponding Editor: Yu Lei.

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Vu, D.D., Bui, T.T.X., Nguyen, M.T. et al. Genetic diversity in two threatened species in Vietnam: Taxus chinensis and Taxus wallichiana . J. For. Res. 28, 265–272 (2017). https://doi.org/10.1007/s11676-016-0323-1

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  • DOI: https://doi.org/10.1007/s11676-016-0323-1

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