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Establishment of high frequency shoot regeneration system in Himalayan poplar (Populus ciliata Wall. ex Royle) from petiole explants using Thidiazuron cytokinin as plant growth regulator

Journal of Forestry Research volume 26, pages 651–656 (2015)Cite this article

Abstract

Populus species are important resources for industry and in scientific study on biological and agricultural systems. Our objective was to enhance the frequency of plant regeneration in Himalayan poplar (Populus ciliata wall. ex Royle). The effect of TDZ alone and in combination with adenine and NAA was studied on the regeneration potential of petiole explants. The explants were excised from Himalayan poplar plants grown in glass-houses. After surface sterilization the explants were cultured on shoot induction medium. High percentage shoot regeneration (86 %) was recorded on MS medium supplemented with 0.004 mg L−1 TDZ and 79.7 mg L−1 adenine. The regenerated shoots for elongation and multiplication were transferred to MS + 0.5 mg L−1 BAP + 0.2 mg L−1 IAA + 0.3 mg L−1 GA3. Root regeneration from shoots developed in vitro was observed on MS medium supplemented with 0.10 mg L−1 IBA. Himalayan poplar plantlets could be produced within 2 months after acclimatization in a sterile mixture of sand and soil. We developed a high efficiency plant regeneration protocol from petiole explants of P. ciliata.

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References

  • Balatinecz JJ, Kretschmann DE, Leclercq A (2001) Acheivements in the utilization of poplar wood-guideposts for the future. For Chron 77:265–269

    Article  Google Scholar 

  • Charenon J, Taris B (1960) Remarques sur la structure des tissus neoformes et l’apparition d’organes specializes chez quatre cultivars de Populus et chez Salix alba cultives in vitro. CR Acad Sci 251:2070–2071

    Google Scholar 

  • Cheema GS (1989) Somatic embryogenesis and plant regeneration from cell suspension and tissue cultures of mature Himalayan poplar (Populus ciliata). Plant Cell Rep 8:124–127

    Article  CAS  PubMed  Google Scholar 

  • Confalonieri M, Balestrazzi J, Bisoffi S, Carbonera D (2003) In vitro culture and genetic engineering of Populus spp. synergy for forest tree improvement. Plant Cell Tissue Organ Cult 72:109–138

    Article  CAS  Google Scholar 

  • Costache IM, Lowe KC, Davey MR, Power JB (1995) Improved micropropagation of Populus spp. by Pluronic F-68. Plant Growth Regul 17:233–239

    Article  Google Scholar 

  • Cseke LJ, Cseke SB, Podila GK (2007) High efficiency poplar transformation. Plant Cell Rep 26:1529–1538

    Article  CAS  PubMed  Google Scholar 

  • Dai WH, Cheng ZM, Sargent W (2003) Plant regeneration and Agrobacterium-mediated transformation of two elite aspen hybrid clones from in vitro leaf tissue. In vitro Cell Dev Biology-Plant 39:6–11

    Article  Google Scholar 

  • De Block M (1990) Factors influencing the tissue culture and Agrobacterium tumefaciens-mediated transformation of hybrid aspen and poplar clones. Plant Physiol 93:1110–1116

    Article  PubMed Central  PubMed  Google Scholar 

  • Dong SX, Ning ZX, Yuan SJ, Liu XQ, Zhang S (1996) Effects of growth regulators on leaf callus induction and plantlet regeneration in Populus tomentosa. Ningxia J Agric For Sci Technol 6:18–20

    Google Scholar 

  • Gangoo SK, Khurana DK (2002) In vitro multiplication of poplars: effects of growth regulators and genotypes. Appl Biol Res 4:11–16

    Google Scholar 

  • Gomez KA, Gomez AA (1984) Statistical procedures for agricultural research. John Wiley and Sons, New York

    Google Scholar 

  • Gosukonda RM, Beyl CA, Zipf A, Sharma GC (1998) In vitro callus initiation and multiple shoot production of Populus deltoides. Plant Growth Regul Soc Am 26:79–93

    CAS  Google Scholar 

  • Huttenman CA, Preece JE (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell, Tissue Organ Cult 33:105–119

    Article  Google Scholar 

  • Jafari MA, Kiss J, Gergacz J, Heszky LE (1995) High efficiency callus induction and plant regeneration in petiole culture of four poplar genotypes. Acta Biol Hung 46:51–59

    CAS  PubMed  Google Scholar 

  • Kang W, Zheng J, Lıu KY, Peng JX, Hong HZ (2006) Study on plant regeneration of excised leaf from Populus deltoids (I-63 × I-69). J Wuhan Bot Res 24:83–86

    Google Scholar 

  • Ledbetter DI, Preece JE (2004) Thidiazuron stimulates adventitious shoot production from Hydrangea quercifolia Bartr. Leaf explants. Sci Hortic 101:121–126

    Article  CAS  Google Scholar 

  • Leple JC, Brasileiro ACM, Michel MF, Delmotte F, Jouanin L (1992) Transgenic poplars: expression of chimaeric genes using four different constructs. Plant Cell Rep 11:137–141

    Article  CAS  PubMed  Google Scholar 

  • Liu CZ, Murch SJ, Demerdash MEL, Saxena PK (2003) Regeneration of the Egyptian medicinal plant Artemisia judaica L. Plant Cell Rep 21:525–530

    CAS  PubMed  Google Scholar 

  • Ma C, Strauss SH, Meilan R (2004) Agrobacterium-mediated transformation of the genome sequenced poplar clone, Nisqually-1 (Populus trichocarpa). Plant Mol Biol Rep 22:1–9

    Article  Google Scholar 

  • Malik KA, Saxena PK (1992) Thidiazuron induces high frequency shoots regeneration in intact seedlings of pea (Pisum sativum), Chickpea (Cicer arietinum) and lentil (Lens culinaris). Aust J Plant Physiol 19:731–740

    Article  CAS  Google Scholar 

  • Mathes MC (1964) Culture of isolated triploid aspen tissue. For Sci 10:35–38

    Google Scholar 

  • Mehra PN, Cheema GS (1980) Clonal multiplication in vitro of Himalayan poplar (Populus ciliata). Phytomorphology 30:336–343

    Google Scholar 

  • Mithila J, Hall JC, Victor JMR, Saxena PK (2003) Thidiazuron induces shoot organogenesis at low concentration and somatic embryogenesis at high concentration on leaf and petiole explants of African violet (Saintpaulia ionantha WEndl.). Plant Cell Rep 21:408–414

    CAS  PubMed  Google Scholar 

  • Murashige T, Skoog F (1962) Revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Planta 15:211–218

    Article  Google Scholar 

  • Murthy BNS, Murch SJ, Saxena PK (1998) Thidiazuron: a potent regulator of in vitro plant morphogenesis. In Vitro Cell Dev Biol 34:267–275

    Article  CAS  Google Scholar 

  • Noel N, Leple JC, Pilate G (2002) Optimization of in vitro micropropagation and regeneration for Populus X interamericana and Populus X euramericana hybrids (P. deltoides, P. trichocarpa, and P. nigra). Plant Cell Rep 20:1150–1155

    Article  CAS  Google Scholar 

  • Park YG, Son SH (1988) In vitro organogenesis and somatic embryogenesis from punctured leaf of Populus nigra × P. maximowiczii. Plant Cell, Tissue Organ Cult 15:95–105

    Article  CAS  Google Scholar 

  • Park S, Oh S, Han KH (2004) Large scale computational analysis of poplar ESTs reveals the repertoire and unique features of expressed genes in the poplar genome. Mol Breed 14:429–440

    Article  CAS  Google Scholar 

  • Pelah D, Kaushik RA, Mizrahi Y, Sitrit Y (2002) Organogenesis in the vine cactus Selenicereus megalanthus using thidiazuron. Plant cell Tissue and Organ Cult 71:81–84

    Article  CAS  Google Scholar 

  • Peternel S, Gabrovsek K, Gogala N, Regvar M (2009) In vitro propagation of European aspen (Populus tremula L.) from axillary buds via organogenesis. Sci Hortic 121:109–112

    Article  CAS  Google Scholar 

  • Phan CT, Jorgensen J, Jouve L, Hausman JF, Polle A, Teichmann T (2004) Micropropagation of Populus euphratica Olivier. Belg J Bot 137:175–180

    Google Scholar 

  • Rishi AS, Nelson ND, Goyal A (2001) Genetic modification for improvement of Populus. Physiol Mol Biol Plants 7:7–21

    Google Scholar 

  • Schnoor JL (2000) Phytostabalization of metal using hybrid poplar tree. In: Raskin I, Ensley BD (eds) Pytoremediation of toxic metals: using plant to clean up the environment. John Wiley and Sons Inc, New York, pp 133–150

    Google Scholar 

  • Schween G, Schwenkel HG (2002) In vitro regeneration in Primula Sp. Via organogenesis. Plant Cell Rep 20:1006–1010

    Article  CAS  Google Scholar 

  • Shen HL, Wantanabe S, Ide Y (1998) Establishment of callus culture system of Populus euphratica, Populus alba cv. Pyramidalis and Populus maximowiczii X Populus plantierensis. Bull Tokyo Univ For 99:19–26

    Google Scholar 

  • Son SH, Hall RB (1990) Plant regeneration capacity of callus derived from leaf, stem and root segments of Populus alba X Populus grandidentata michx. Plant Cell Rep 9:344–347

    Article  CAS  PubMed  Google Scholar 

  • Thakur AK, Srivatava DK (2006) High efficiency plant regeneration from leaf explants of male Himalayan poplar (Populus ciliata wall.). In vitro Cell Dev Biol Plant 42:144–147

    Article  CAS  Google Scholar 

  • Thakur AK, Sharma S, Srivastava DK (2008) Direct organogenesis and plant regeneration from petiole explant of male Himalayan poplar (Populus ciliata wall.). Phytomorphology 58:49–55

    Google Scholar 

  • Thakur AK, Saraswat A, Srivastava DK (2012) In vitro plant regeneration through direct organogenesis & in Populus deltoids clone G48 from petiole explants. J Plant Biochem Biotechnol 21:23–29

    Article  CAS  Google Scholar 

  • Tsvetkov I, Hausman JF, Jouve L (2007) Thidiazuron induced regeneration in root segment of white poplar (P. alba L.). Bulg J Agric Sci 13:623–626

    Google Scholar 

  • Venverloo CJ (1973) Formation of adventitious organs in cytokinin induced formation of leaves and shoots in callus cultures of Populus nigra L. Acta Bot Neerl 22:390–398

    Article  CAS  Google Scholar 

  • Walter MW, Grima-pettenati J, Grand C, Boudet AM, Lamb C (1988) Cinnamyl alcohol dehydrogenase, a molecular marker specific for lignin synthesis: cDNA cloning and mRNA induction by fungal elicitor. Proceedings of the National Academy of Sciences, vol 85, pp 5546–5550

  • Winton LL (1968) Plantlets from aspen tissue culture. Science 160:1234–1235

    Article  CAS  PubMed  Google Scholar 

  • Yevtushenko PD, Misra S (2010) Efficient Agrobacterium-mediated transformation of commercial hybrid poplar Populus nigra X Populus maximowiczii. Plant Cell Rep 29:211–221

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Biotechnology, Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, Solan, 173230, HP, India

    G. Aggarwal, A. Gaur & D. K. Srivastava

  2. Department of Food Technology, Desh Bhagat University, Mandi Gobindgarh, 147301, Pb, India

    G. Aggarwal

Authors
  1. G. Aggarwal
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  2. A. Gaur
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  3. D. K. Srivastava
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Correspondence to G. Aggarwal.

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The online version is available at http://www.springerlink.com

Corresponding editor: Zhu Hong

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Aggarwal, G., Gaur, A. & Srivastava, D.K. Establishment of high frequency shoot regeneration system in Himalayan poplar (Populus ciliata Wall. ex Royle) from petiole explants using Thidiazuron cytokinin as plant growth regulator. J. For. Res. 26, 651–656 (2015). https://doi.org/10.1007/s11676-015-0048-6

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  • DOI: https://doi.org/10.1007/s11676-015-0048-6

Keywords

  • In vitro regeneration
  • Petiole explants
  • Growth regulator
  • Thidiazuron
  • Populus ciliata