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Sequence-related amplified polymorphism primer screening on Chinese fir (Cunninghamia lanceolata (Lamb.) Hook)

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Abstract

Chinese fir (Cunninghamia lanceolata (Lamb.) Hook) is one of the most important coniferous tree species used for timber production in China. Here, we conducted a sequence-related amplified polymorphism (SRAP) primer screening assay with a total of 594 primer combinations, using 22 forward and 27 reverse primers on four representative Chinese fir genotypes. The obtained results indicated that Chinese fir genomic DNA has a notable amplification bias on the employed forward or reverse primer nucleotides (3′ selection bases). Out of the tested primer sets, 35 primer combinations with clearly distinguished bands, stable amplification, and rich polymorphism were selected and identified as optimal primer sets. These optimal primer pairs gave a total of 379 scorable bands, including 265 polymorphic bands, with an average of 10.8 bands and 7.6 polymorphic bands per primer combination. The produced band number for each optimal primer set ranged from 7 to 14 with a percentage of polymorphic bands spanning from 33.3 to 100.0 %. These primer combinations could facilitate the next SRAP analysis assays in Chinese fir.

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Acknowledgments

This research was supported by the National Natural Sciences Foundation of China (No. 31200506) and the Special Fund for Forest Scientific Research in the Public Welfare (No. 201404127).

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    Authors and Affiliations

    1. Guangdong Academy of Forestry, Guangzhou, 510520, The People’s Republic of China

      Huiquan Zheng, Dehuo Hu & Ruping Wei

    2. National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, 100083, The People’s Republic of China

      Hongjing Duan & Yun Li

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    1. Huiquan Zheng
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    2. Hongjing Duan
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    3. Dehuo Hu
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    Correspondence to Dehuo Hu.

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    Huiquan Zheng and Hongjing Duan contributed equally to this work.

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    Zheng, H., Duan, H., Hu, D. et al. Sequence-related amplified polymorphism primer screening on Chinese fir (Cunninghamia lanceolata (Lamb.) Hook). J. For. Res. 26, 101–106 (2015). https://doi.org/10.1007/s11676-015-0025-0

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