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Purification of four strains of endophytic fungi from Astragalus and their optimized liquid fermentations

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Abstract

This study was designed to isolate endophytic fungi from A. mongholicus (growing in northeast China) to determine whether they can produce bioactive metabolites. Four strains of endophytic fungi (strains 16, 17, 23 and 75) were successfully isolated from A. mongholicus using the surface disinfection method. According to ITS-rDNA sequences analysis, strains 16 and 75 were identified as Fusarium oxysporum, and strains 17 and 23 were identified as Bionectria ochroleuca. We applied the Box-Behnken design (BBD) to optimize the liquid fermentation conditions and obtain the maximum cell dry weight (CDW) yield. Optimal parameters were obtained under the following experimental conditions: temperature of 28°C, potato dextrose agar (PDA) liquid medium of 80 mL and rotation speed of 150 rpm. The four isolated endophytic fungi did not produce astragalosides I–IV, flavonoids or polysaccharides. Isolation of additional species of endophytic fungi from A. mongholicus and determination of their capacity to produce biologically active substances are subjects in need of further research.

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References

  • Aly AH, Debbab A, Proksch P. 2011. Fungal endophytes: unique plant inhabitants with great promises. Applied Microbiology and Biotechnology, 90: 1–17.

    Article  Google Scholar 

  • Chantasinghab D, Kitikhunb S, Eurwilaichitrb L, Uengwetwanitb T, Pootanakita K. 2011. Functional expression in Beauveria bassiana of a chitinase gene from Ophiocordyceps unilateralis, an ant-pathogenic fungus. Biocontrol Science and Technology, 21: 677–686.

    Article  Google Scholar 

  • Chen XY. 2007. Primary studies on correlation of endophyte isolated from panax quinquefolium and gingsenoside. Dissertation, Heilongjiang University of Chinese Medicine.

    Google Scholar 

  • Cho WCS, Leung KN. 2007. In vitro and in vivo immunomodulating and immunorestorative effects of Astragalus membranaceus. Journal of Ethnopharmacology, 113: 132–141.

    Article  PubMed  Google Scholar 

  • Chu DT, Lepe-Zuniga J, Wong WL, LaPushin R, Mavligit GM. 1988. Fractionated extract of Astragalus membranaceus, a Chinese medicinal herb, potentiates LAK cell cytotoxicity generated by a low dose of recombinant interleukin-2. Journal of Clinical & Laboratory immunology, 26: 183–187.

    CAS  Google Scholar 

  • Cui YN, Yi DW, Bai XF, Sun BS, Zhao YQ, Zhang YX. 2012. Ginkgolide B produced endophytic fungus (Fusarium oxysporum) isolated from Ginkgo biloba. Fitoterapia, 83: 913–920.

    Article  CAS  PubMed  Google Scholar 

  • de Barros BS, da Silva JP, de Souza Ferro JN, Agra IK, de Almeida Brito F, Albuquerque ED, Caetano LC, Barreto E. 2011. Methanol extract from mycelium of endophytic fungus Rhizoctonia sp. induces antinociceptive and anti-inflammatory activities in mice. Journal of Natural Medicines, 65: 526–531.

    Article  PubMed  Google Scholar 

  • Harper JK, Strohmeier M, Grant DM. 2007. Pursuing structure in microcrystalline solids with independent molecules in the unit cell using 1H-13C correlation data. Journal of Magnetic Resonance, 189: 20–31.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Ji RJ. 2012. Studies on Astragalus biological characteristics and functions and progress of modern pharmaceutics. Chinese Journal of Clinical Rational Drug Use, 5: 176–177.

    Google Scholar 

  • Kumar A, Patil D, Rajamohanan PR, Ahmad A. 2013. Isolation, purification and characterization of vinblastine and vincristine from endophytic fungus Fusarium oxysporum isolated from Catharanthus roseus. Plos One, 8: e71805.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Kusari S, Lamshöft M, Zühlke S, Spiteller M. 2008. An endophytic fungus from Hypericum perforatum that produces hypericin. Journal of Natural Products, 71: 159–162.

    Article  CAS  PubMed  Google Scholar 

  • Liu K, Ding X, Deng B, Chen W. 2009. Isolation and characterization of endophytic taxol-producing fungi from Taxus chinensis. Journal of Industrial Microbiology & Biotechnology, 36: 1171–1177.

    Article  CAS  Google Scholar 

  • Liu XL, Huang KH, Zhou JZ, Meng L, Wang Y, Zhang LX. 2012. Identification and antibacterial characteristics of an endophytic fungus Fusarium oxysporum from Lilium lancifolium. Letters in Applied Microbiology, 55: 399–406.

    Article  Google Scholar 

  • Luangsa-ard JJ, Hywel-Jones NL, Samson RA. 2004. The polyphyletic nature of Paecilomyces sensu lato based on 18S-generated rDNA phylogeny. Mycologia, 96: 773–780.

    Article  CAS  PubMed  Google Scholar 

  • Ma W, Sun LY, Zhang XW, Jia YS, Kong XJ, Xie JQ. 2012. Preliminary studies on active constituent in endogenous fungal fermented liquid of Radix Astragali. Acta Chinese Medicine and Pharmacology, 3: 041.

    CAS  Google Scholar 

  • Ou LC, Wang XH, Zhang CH. 2013. Production and characterization of dragon’s blood from leaf blades of Dracaena cambodiana elicited by Fusarium proliferatum. Industrial Crops and Products, 45: 230–235.

    Article  CAS  Google Scholar 

  • Park YH, Lee SG, Ahn DJ, Kwon, TR, Park SU, Lim HS, Bae H. 2012. Diversity of fungal endophytes in various tissues of Panax ginseng Meyer cultivated in Korea. Journal of ginseng research, 36: 211.

    Article  PubMed Central  PubMed  Google Scholar 

  • Samaga PV, Rai VR, Rai KML. 2013. Bionectria ochroleuca NOTL33-an endophytic fungus from Nothapodytes foetida producing antimicrobial and free radical scavenging metabolites. Ann Microbiol, 64: 275–285.

    Article  Google Scholar 

  • Strobel G, Daisy B, Castillo U, Harper J. 2004. Natural Products from Endophytic Microorganisms. Journal of Natural Products, 67: 257–268.

    Article  CAS  PubMed  Google Scholar 

  • Strobel GA. 2002. Rainforest endophytes and bioactive products. Critical Reviews in Biotechnology, 22: 315–333.

    Article  CAS  PubMed  Google Scholar 

  • Sun Y, M XB, Liu JX. 2012. Compounds from fraction with cardiovascular activity of Chrysanthemum indicum. Chinese Journal of Chinical Mater Medicine, 37: 61–65.

    CAS  Google Scholar 

  • Sun Y, Wang Q. 2006. Identification of secondary metabolites produced by endophytic fungus from Astragalus membranaceus and characterization of their antibacterial activity. Journal of Fungal Research, 4: 47–51.

    CAS  Google Scholar 

  • Tan RX, Zou WX. 2001. Endophytes: a rich source of functional metabolites. Natural Product Reports, 18: 448–459.

    Article  CAS  PubMed  Google Scholar 

  • Wang QX, Li SF, Zhao F, Dai HQ, Bao L, Ding R, Gao H, Zhang LX, Wen HA, Liu HW. 2011. Chemical constituents from endophytic fungus Fusarium oxysporum. Fitoterapia, 82: 777–781.

    Article  CAS  PubMed  Google Scholar 

  • Zhou F, Zhang HC, Liu R, An ZP. 2012b. Isolation, identification and antimicrobial activities of endophytic fungi from Astragalus membranaceus. Food Science and Technology, 1:011.

    Google Scholar 

  • Zhou F, Zhang HC, Liu R, Zhang YF. 2012a. Isolation and biological evaluation of secondary metabolites of endophytic fungus Aspergillus sp. from Astragalus membranaceus. Chinese Journal of Experimental Traditional Medical Formulae, 4: 038.

    Google Scholar 

  • Zu YG, Yan MM, Fu YJ, Liu W, Zhang L, Gu CB, Efferth T. 2009. Determination and quantification of astragalosides in Radix Astragali and its medicinal products using LC-MS. Journal of Separation Science, 32: 517–52.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. College of Pharmacy, Heilongjiang University of Chinese Medicine, 24 Heping Road, Harbin, Heilongjiang, 150040, China

    Wei Ma, Xiubo Liu, Leiming Zhang, Weichao Ren, Ning Zhang & Xiwu Zhang

  2. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040, PR China

    Wei Ma, Xiubo Liu & Jiao Jiao

  3. College of Forestry, Northeast Forestry University, 26 Hexing Road, Harbin, Heilongjiang, 150040, China

    Ling Ma

  4. The First Affiliated Hospital of Qiqihar Medical College, 26 Xiangyang Road, Qiqihar, Heilongjiang, 161000, China

    Xiangjun Kong

Authors
  1. Wei Ma
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  2. Xiubo Liu
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  3. Jiao Jiao
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  4. Leiming Zhang
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  5. Weichao Ren
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  6. Ling Ma
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  7. Xiangjun Kong
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  8. Ning Zhang
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  9. Xiwu Zhang
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Corresponding author

Correspondence to Ling Ma.

Additional information

These authors contributed equally to this work.

Project funding: This work was supported by grants from the Key Program of Natural Science Foundation of State (Grant No. 81274010), Heilongjiang province outstanding youth fund (Grant No. JC201101) and Talent fund of Heilongjiang University of Chinese Medicine Talent Fund.

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Ma, W., Liu, X., Jiao, J. et al. Purification of four strains of endophytic fungi from Astragalus and their optimized liquid fermentations. Journal of Forestry Research 25, 701–706 (2014). https://doi.org/10.1007/s11676-014-0510-x

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  • DOI: https://doi.org/10.1007/s11676-014-0510-x

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