Journal of Forestry Research

, Volume 23, Issue 4, pp 583–592 | Cite as

Low temperature, IBA concentrations and optimal time for adventitious rooting of Eucalyptus benthamii mini-cuttings

  • Gilvano Ebling Brondani
  • Francisco José Benedini Baccarin
  • Heron Wilhelmus de Wit Ondas
  • José Luiz Stape
  • Antonio Natal Gonçalves
  • Marcilio de Almeida
Original Paper


Eucalyptus benthamii is a forest species of economic interest that has difficulty with seed production and also is considered to have difficulty with adventitious rooting using propagation techniques, such as cutting or mini-cutting. We aimed to assess the adventitious rooting percentage under different storage times in low temperatures and at various IBA (indole-3-butyric acid) concentrations to determine the optimal time of permanence for rooting Eucalyptus benthamii minicuttings in a greenhouse. Shoots collected from mini-stumps cultivated in a semi-hydroponic system were used to obtain the mini-cuttings. For the first experiment, the mini-cuttings were stored at 4°C for 0 (immediate planting), 24, 48, 72, 96 and 120 h. The second experiment evaluated the rooting dynamic to determine the optimal time of permanence for minicuttings in a greenhouse. The basal region of the mini-cutting was treated with various IBA solutions: 0 (free of IBA), 1,000, 2,000, 3,000 and 4,000 mg·L−1. Every seven days (0 (immediate planting), 7, 14, 21 and 28 days), destructive sampling of the mini-cuttings was performed to evaluate the histology of the adventitious rooting. Eucalyptus benthamii minicuttings should be rooted immediately after the collection of the shoots. The 2,000 mg·L−1 IBA concentration induced a greater speed and percentage of adventitious rooting, and an interval of 35 to 42 days was indicated for permanence of the mini-cuttings in the greenhouse. Exposure to low temperature induced adventitious root formation with diffuse vascular connections.


rhizogenesis plant cloning mini-cutting technique histological analysis indole-3-butyric acid 


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Copyright information

© Northeast Forestry University and Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Gilvano Ebling Brondani
    • 1
  • Francisco José Benedini Baccarin
    • 2
  • Heron Wilhelmus de Wit Ondas
    • 3
  • José Luiz Stape
    • 4
  • Antonio Natal Gonçalves
    • 2
  • Marcilio de Almeida
    • 3
  1. 1.Department of Forest EngineeringFederal University of Mato Grosso (UFMT)Cuiabá, Mato GrossoBrazil
  2. 2.Department of Forest Sciences, “Luiz de Queiroz” College of AgricultureUniversity of São Paulo (ESALQ/USP)Piracicaba, São PauloBrazil
  3. 3.Department of Biological Sciences, “Luiz de Queiroz” College of AgricultureUniversity of São Paulo (ESALQ/USP)Piracicaba, São PauloBrazil
  4. 4.Department of Forest and Environmental SciencesNorth Carolina State UniversityCarolinaUSA

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