Abstract
A continuous stirred-tank reactor (CSTR) process with granular activated carbon (GAC) was developed for fermentation hydrogen production from molasses-containing wastewater by mixed microbial cultures. Operation at 35°C, an initial biomass of 17.74 g·L−1 and hydraulic retention time (HRT) of 6 h, the CSTR reactor presented a continuous hydrogen production ability of 5.9 L·d−1 and the biogas was free of methane throughout the experiment. Dissolved fermentation products were predominated by ethanol and acetate acid, with smaller quantities of propionic acid, butyric acid and valeric acid. It was found that GAC could make the immobilized system durable and stable in response to organic load impacting and low pH value. When the organic loading rate (OLR) ranged from 8 kgCOD/(m3d) to 4 kgCOD/(m3d), stable ethanol-type fermentation was formed, and the ethanol and acetate concentrations account for 89% of the total liquid products.
Similar content being viewed by others
References
Benemann J. 1996. Hydrogen biotechnology: progress and prospects. Nat Biotechnol, 14: 1101–1103.
Das D, Veziroglu TN. 2001. Hydrogen production by biological process: a survey of literature. Int J Hydrogen Energy, 26:13–28.
Gavala HN, Skiadas IV, Ahring BK. 2006. Biological hydrogen production in suspended and attached growth anaerobic reactor systems. Int J Hydrogen Energy, 31: 1164–1175.
Han SK, Shin HS. 2004. Biohydrogen production by anaerobic fermentation of food waste. Int J Hydrogen Energy, 29: 569–577
Jin B, Van Leeuwen HJ, Patel B, Yu Q. 1998. Utilization of starch processing wastewater for production of microbial biomass protein and fungal α-amylase by aspergillus oryzae. Bioresource Technology, 66: 201–206.
Kataoka N, Miya A, Kiriyama K. 1997. Studies on hydrogen production by continuous culture system of hydrogen producing anaerobic bacteria. Water Sci Technol, 36: 41–47.
Kim JO, Kim YH, Ryu JY, Song BK, Kim IH, Yeom SH. 2005. Immobilization methods for continuous hydrogen gas production biofilm formation versus granulation. Process Biochem, 40: 1331–1337.
Lee DY, Li YY, Noike T, Cha GC. 2008. Behavior of extracellular polymers and bio-fouling during hydrogen fermentation with a membrane bioreactor. Journal of Membrane Science, 322(1): 13–18.
Lee KS, Wu JF, Lo, YS, Lo YC, Lin PJ, Chang JS. 2004. Anaerobic hydrogen production with an efficient carrier-induced granular sludge bed bioreactor. Biotechnol Bioeng, 87: 648–657.
Levin DB, Pitt L, Love M. 2004. Biohydrogen production: prospects and imitations to practical application. Int J Hydrogen Energy, 29(2): 173–185.
Logan BE, Oh SE, Kim IS, Van Ginkel S. 2002. Biological hydrogen production measured in batch anaerobic respirometers. Environ Sci Technol, 36: 530–2535.
Patrick CH, Benemann JR. 2000. Biological hydrogen production: fundamentals and limiting process. Int J Hydrogen Energy, 27: 1185–1193.
Ren N, Qin Z, Li J. 2003. Comparision and Analysis of Hydrogen Production Capacity with Different Acidogenic Fermentative Microflora. Environmental Science, 24(1): 70–74.
Tao Y, Chen Y, Wu Y, He Y, Zhou Z. 2007. High hydrogen yield from a two-step process dark and photo-fermentation of sucrose. Int J Hydrogen Energy, 32(2): 200–206.
Teplyakow VV, Gassanova LG, Sostina EG, Slepova EV, Modigell M, et al., Netrusov AI. 2002. Lab-scale bioreactor integration with active membrane system for hydrogen production:experience and prospects. Int J Hydrogen Energy, 27(11–12): 1149–11551.
Ueno Y, Otsuka S. Morimoto M. 1996. Hydrogen production from industrial wastewater by anaerobic microflora in chemostat culture. Journal of Fermentation and Bioengineering, 83: 194–197.
Wu SY, Lin CN, Chang JS. 2003. Hydrogen production with immobilized sewage sludge in three-phase fluidized-bed bioreactors. Biotechnol Prog, 9(3): 828–832.
Author information
Authors and Affiliations
Corresponding author
Additional information
Foundation project: This study was supported by the National Hi-Tech R&D Program (863 Program), Ministry of Science&Technology, China (Grant No. 2006AA05Z109) and Shanghai Science and Technology Bureau (Grant No.071605122) and Educated programme of excellent doctor of Southeast Forestry University (GRAP09)
Rights and permissions
About this article
Cite this article
Han, W., Chen, H., Yao, X. et al. Biohydrogen production with anaerobic sludge immobilized by granular activated carbon in a continuous stirred-tank. Journal of Forestry Research 21, 509–513 (2010). https://doi.org/10.1007/s11676-010-0107-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11676-010-0107-y