Journal of Forestry Research

, 19:219 | Cite as

Identification of mangrove endophytic fungus 1403 (Fusarium proliferatum) based on morphological and molecular evidence

  • Zhong-shan Cheng
  • Wen-cheng Tang
  • Zhi-jian Su
  • Yi Cai
  • Shi-feng Sun
  • Qi-jin Chen
  • Fang-hai Wang
  • Yong-cheng Lin
  • Zhi-gang She
  • LLP Vrijmoed
Research Paper

Abstract

A mangrove endophytic fungus 1403 isolated from the South China Sea Coast, which is able to produce griseofulvin and anthracenediones under submerged fermentation, was compared with Fusarium genus with the similar morphological characters such as elongated, microconidium-producing conidiophores, ovoid microconidia and straight to slightly curved macroconidia. It was found that the fungus 1403 resembles pathogenic F. verticillioides (teleomophy Gibberella moniliforme) in the production of false head or chains and abundant microconidia on the aerial mycelium, but different in the occasional formation of polyphialides with relatively long as well as short monophialides, in its typical coiled hyphae and mycelia fusion. Through maximum Parsimony and Bayesian analyses, the fungus 1403 was further compared with some similar Fusarium species. The results indicated that this endophyte was identified as Fusarium proliferatum based on the analyses of partial 18S and 28S rDNA genes, ITS region, and EF-1α gene.

Keywords

Fusarium Mangrove endophyte Morphology Phylogeny 

References

  1. Bacon CW, Williamson JW. 1992. Interactions of Fusarium moniliforme, its metabolites and bacteria with maize. Mycopathologia, 117: 65–71.CrossRefGoogle Scholar
  2. Calendini F, Martin JF. 2005. PaupUP v1.0.3.1, A free graphical frontend for Paup* Dos software.Google Scholar
  3. Gams W, Klamer M, O’Donnell K. 1999. Fusarium miscanthi sp. nov. from Miscanthus litter. Mycologia, 91: 263–268.CrossRefGoogle Scholar
  4. Geiser D, Del Mar Jiménez-Gasco M, Kang S, et al. 2004. FUSARIUM-ID v.1.0: A DNA sequence database for identifying Fusarium. Eur J Plant Pathol, 110: 473–479.CrossRefGoogle Scholar
  5. Gilbert GS, Mejía-Chang M. 2002. Fungal diversity and plant disease in mangrove forests: salt excretion as a possible defense mechanism. Oecologia, 132: 278–285.CrossRefGoogle Scholar
  6. Guo LD, Hyde KD, Liew ECY. 2002. Identification of endophytic fungi from Livistona chinensis based on morphology and rDNA sequences. New Phytol, 147: 617–630.CrossRefGoogle Scholar
  7. Hallmann, J, Sikora RA. 1994. Occurrence of plant parasitic nematodes and endophytic fungi in tomato plants in Kenya and their role as mutualistic synergists for biological control of root-knot nematodes. Int J Pest Manage, 40:321–325.CrossRefGoogle Scholar
  8. Huelsenbeck JP, Ronquist F. 2001. MRBAYES:Bayesian inference of phylogenetic trees. Bioinformatics, 17: 754–755.PubMedCrossRefGoogle Scholar
  9. Huss MJ, Campbell CL, Jennings DB, et al. 1996. Isozyme variation among biological species in the Gibberella fujikuroi species complex (Fusarium section Liseola). Appl Environm Microbiol, 63: 3750–3756.Google Scholar
  10. Ignazi C, Linda MK. 1999. A method for designing primer sets for speciation studies in Filamentous Ascomycetes. Mycologia, 91(3): 553–556.CrossRefGoogle Scholar
  11. Jiang Guangce, Lin Yongcheng, Zhou Shining, Vrijmoed LL, Jones EBG. 2000. Studies on the secondary metabolites of mangrove fungus No. 1403 from the South China Sea. Acta Sci Nat Univ Sunyatseni, 39(6):68–72. (in Chinese)Google Scholar
  12. Kathiresan K, Bingham BL. 2001. Biology of mangroves and mangrove ecosystems. Adv Mar Biol, 40: 81–251.CrossRefGoogle Scholar
  13. Kerényi Z, Zeller KZ, Hornok L, Leslie JF. 1999. Molecular standardization of mating type terminology in the Gibberella fujikuroi species complex. Appl Environ Microbiol, 65: 4071–4076.PubMedGoogle Scholar
  14. Láday M, Juhász Á, Mulè G., Moretti A, Szécsi Á, Logrieco A. 2004. Mitochondrial DNA variability in Fusarium proliferatum (Gibberella intermedia). Eur J Plant Pathol, 110: 563–571.CrossRefGoogle Scholar
  15. Lin YC, Wu XY, Feng S, Jiang GC, Luo JH, Zhou SN, Vrijmoed LLP, Jones EBG, Krohn K, Steingröver K, Zsila F. 2001. Five unique compounds: xyloketals from mangrove fungus Xylaria sp. from the South China Sea coast. J Org Chem, 66:52–62.Google Scholar
  16. Lin YC, Wu XY, Deng ZJ, Wang J, Zhou SN, Vrijmoed LLP, Jones EBG. 2002. The metabolites of the mangrove fungus Verruculina enalia No. 2606 from a salt lake in the Bahamas. Phytochem, 59:469–471.CrossRefGoogle Scholar
  17. Marasas WFO, Nelson PE, Toussoum TA. 1984. Toxigenic Fusarium species Identification and Mycotoxicology. University Park, PA: The Pennsylvania State University Press.Google Scholar
  18. Nelson PE, Desiardins AE, Plattner RD. 1993. Fumonisins, mycotoxins produced by Fusarium species: biology, chemistry and significance. Annu Rev Phytopathol, 31: 233–251.PubMedCrossRefGoogle Scholar
  19. Nirenberg HI, O’Donnell K. 1998. New Fusarium species and combinations within the Gibberella fujikuroi species complex. Mycologia, 90: 434–458.CrossRefGoogle Scholar
  20. O’Donnell K. 2000. Molecular phylogeny of the Nectria haematococca-Fusarium solani species complex. Mycologia, 92: 919–938.CrossRefGoogle Scholar
  21. O’Donnell K, Cigelnik, E. 1997. Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus Fusarium are nonorthologous. Mol Phylo Evol, 1:1–14.Google Scholar
  22. O’Donnell K, Cigelnik E, Nirenberg HI. 1998. Molecular systematics and phylogeograhy of the Gibberella fujikuroi species complex. Mycologia, 90: 465–493.CrossRefGoogle Scholar
  23. O’Donnell K, Nirenberg HI, Aoki T, Cigelnik E. 2000. A multigene phylogeny of the Gibberella fujikuroi species complex: Detection of additional phylogenetically distinct species. Mycoscience, 41: 61–78.CrossRefGoogle Scholar
  24. Rheeder, JP, Marasas WGO, Vismer HF. 2002. Production of fumonisin analogs by Fusarium species. Appl Environ Microbiol, 68(5): 2101–2105.PubMedCrossRefGoogle Scholar
  25. Selosse MA, Baudoin E, Vandenkoornhuyse P. 2004. Symbiotic microorganisms, a key for ecological success and protection of plants. C R Biologies, 327: 639–648.PubMedCrossRefGoogle Scholar
  26. Sheadrer CA, Descals E, Kohlmeyer B, Kohlmeyer J, Marvanová L, Padgett D, Porter D, Raja H, Schmit J, Thorton H, Voglymayr H. 2007. Fungal biodiversity in aquatic habitats. Biodivers Conserv, 16: 49–67.CrossRefGoogle Scholar
  27. Stankovic S, Levic J, Petrovic T, Logrieco A, Moreti A. 2007. Pathogenicity and mycotoxin production by Fusarium proliferatum isolated from onion and garlic in Serbia. Eur J Plant Pathol, 118: 165–172.CrossRefGoogle Scholar
  28. Swofford DL 2002. PAUP*: Phylogenetic Analysis Using Parsimony (* and other methods) Version 4.0b10 (Sinauer Associates, Sunderland, MA).Google Scholar
  29. Thompson JD, Gibson TJ, Plewniak F, Jeanmouqin F, Hiqqins DG. 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl Acids Res, 24: 4876–4882.CrossRefGoogle Scholar
  30. Voss KA, Riley RT, Norred WP, Bacon CW, Meredith FI, Howard PC, Planner RD, Collins TF, Hansen DK, Porter JK. 2001. An overview of Rodent toxicities: Liver and Kidney effects of Fumonisins and Fusarium moniliforme. Environ Health Persp, 109(2): 259–266.CrossRefGoogle Scholar
  31. Waalwijk C, De Koning JRA, Baayen RP, Baayen RP, Gams W. 1996. Discordant grouping of Fusarium spp. from sections Elegans, Liseola, Dlaminia based on ribosomal ITS1 and ITS2 sequences. Mycologia, 88(3): 361–368.CrossRefGoogle Scholar
  32. Yuan KP, Vrijmoed LLP, Feng MG. 2005. Survey of coastal mangrove fungi for xylanase production and optimized culture and assay conditions. Acta Microbiologica Sinica, 45(1): 91–96.PubMedGoogle Scholar
  33. Yates IE, Bacon CW, Hinton DM. 1997. Effects of endophytic infection by Fusarium moniliforme on corn growth and cellular morphology. Plant Dis, 81: 723–728.CrossRefGoogle Scholar

Copyright information

© Northeast Forestry University and Springer-Verlag GmbH 2008

Authors and Affiliations

  • Zhong-shan Cheng
    • 1
  • Wen-cheng Tang
    • 1
  • Zhi-jian Su
    • 2
  • Yi Cai
    • 1
  • Shi-feng Sun
    • 1
  • Qi-jin Chen
    • 1
  • Fang-hai Wang
    • 1
  • Yong-cheng Lin
    • 3
  • Zhi-gang She
    • 3
  • LLP Vrijmoed
    • 4
  1. 1.School of Life ScienceSun Yat-sen UniversityGuangzhouP. R. China
  2. 2.School of Pharmaceutical ScienceJinan UniversityGuangzhouP.R. China
  3. 3.School of Chemistry and Chemical EngineeringSun Yat-sen UniversityGuangzhouP.R. China
  4. 4.Department of Biology and ChemistryCity University of Hong KongHongkongChina

Personalised recommendations