Abstract
Solanesol is an important secondary metabolite in Nicotiana tabacum. Distribution of solanesol in Nicotiana tabacum was investigated by High Performance Liquid Chromatography (HPLC) method. The quantitative distribution of solanesol in various organs and tissues of N. tabacum showed that solanesol content, obviously different in all organs, was 6.8, 18.3, 27.5, 45.8, and 68.0 times higher in leaves than that in the stalks, flowers, seeds, fruits and roots, respectively. The contents of solanesol in various parts of leaf, stalk and flower were determined. The content of solanesol in top leaf, middle leaf and bottom leaf gradually decreased (6.124, 5.813 and 5.687 mg·g−1, respectively) and the content of solanesol in various leaf-parts (leaf apex, leaf middle and leaf base) also gradually decreased. The content of solanesol in top stalk was 1.19 times and 1.92 times higher than that in the middle stalk and the bottom stalk, respectively. The content of solanesol in various tissues of stalk (epidermis, cortex and stele) dramatically decreased. The sepal contained higher concentration of solanesol (1.192 mg·g−1) compared to any other parts in flower. The study will provide the base data for the regulation and control of solanesol, moreover, it will provide the scientific evidences for the rational development and utilization of N. tabacum resources.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Cepeda-Benito, A., Reynoso, J.T., Davis, K.W., et al. 2006. Harraid, Interdose interval effects on the development of contextual tolerance to nicotine’s analgesic effects in rats (Rattus norvegicus). Exp. Clin. Psychopharmacol., 14: 180–189.
Crofcheck, C., Loiselle, M., Weekley, J., et al. 2003. Histidine tagged protein recovery from tobacco extract by foam fractionation. Biotechnol. Prog., 19: 680–682.
Daniell, H., Streatfield, S.J., Wycoff, K. 2001. Medical molecular far-ming: production of antibodies, biopharmaceuticals and edible vaccines in plants. Trends plant sci., 6: 219–226.
Douce, D.S., Clench, M.R., Frost, B. 2001. Variations in the estimation of the contribution of environmental tobacco smoke (ETS) to respirable (≤5 mm) indoor air particulates obtained by the use of different analytical methods. J. Environ. Monit., 3: 295–301.
Duan Wengui, Chen Xiaopeng, An Xinnan. 2000. Methods of extract in g solan esol from tobacco. J. Chem. Ind. Forest Products, 34(2): 21–25. (In Chinese)
Kijima, S., Yamatsu, I., Inai, Y., et al. 1979. Hypertension treating agent containing polyprenyl alcohol. USP 4175139.
Küttner, A., Pieper, A., Koch, J., et al. 2005. Influence of coenzyme Q10 and cerivastatin on the flow-mediated vasodilation of the brachial artery: results of the ENDOTACT study. Int. J. Cardiol., 98: 413–419.
Li Chunying, Zhao Chunjian, Zu Yuangang. 2006. Simultaneous determination of solanesol and coenzyme Q10 in the extract of tobacco (Nicotiana tabacum L.) leaves by HPLC. Chin. J. Appl. Chem., 23(5): 524–527. (In Chinese)
Lipshutz, B.H., Lower, A., Berl, V., et al. 2005. An Improved Synthesis of the “Miracle Nutrient” Coenzyme Q10. Org. Lett., 7: 4095–4097.
Masahiro, T., Ryuta, F. 1994. Solanesol and its production. JP 6065128.
Narosimha-Rao, C.V., Prabhu, S.R., Siva-Raju, K. 2000. Influence of plant growth on the distribution of solanesol in tobacco green leaf. Tob. Res., 26: 47–50.
Portakal, O., Özkaya, Ö., ErdenInal, M., et al. 2000. Coenzyme Q10 concentrations and antioxidant status in tissues of breast cancer patients. Clin. Biochem., 33: 279–284.
Rowland, R.L., Latimer, P.H., Giles, J.A. 1956. Flue-cured tobacco. I. Isolation. of solanesol, an unsaturated alcohol. J. Am. Chem. Soc., 78: 4680–4683.
Rüegg, R., Gloor, U., Goel, R.N., et al. 1959. Synthese von ubichinon (45) und ubichinon (50). Helv. Chim. Acta, 42: 2616–2621.
Rundek, T., Naini, A., Sacco, R., et al. 2004. Atorvastatin decreases the coenzyme Q10 level in the blood of patients at risk for cardiovascular disease and stroke. Arch. Neurol., 61: 889–892.
Severson, R.F., Ellington, J.J., Schlotzhauer, P.F., et al. 1977. Gas chetomatographic method for the determination of free and total solanesol in tobacco. J. Chromatogr., 139: 269–282.
Svob-Troje, Z., Frobe, Z., Perovic, D. 1997. Analysis of selected alkaloids and sugars in tobacco extract. J. Chromatogr. A, 775: 101–107.
Tahara, Y., Nagai, M., Kogure, K., et al. 1980. Antiulcer compounds. USP 4221810.
Wang, E., Hall, J.T., Wagner, G.J. 2004. Transgenic Nicotiana tabacum L. with enhanced trichome exudate cembratrieneols has reduced aphid infestation in the field. Mol. Breed., 13:49–57.
Weant, K.A., Smith, K.M. 2005. The role of coenzyme Q10 in heart failure. Ann. Pharmacother., 39:1522–1526.
Whitfield, M.B. 2004. Integrated Bioprocessing of Native and Engineered Components from Tobacco (Nicotiana tabacum L.). Raleigh: Graduate Faculty of North Carolina State University, p25.
Yalcin, A., Kilinc, E., Sagcan, A., et al. 2004. Coenzyme Q10 concentrations in coronary artery disease. Clin. Biochem., 37: 706–709.
Zu, Y.G., Zhao, C.J., Li, C.Y., et al. 2006. A rapid and sensitive LC-MS/MS method for determination of coenzyme Q10 in tobacco (Nicotiana tabacum L.) leaves. J. Sep. Sci., 29: 1607–1612.
Author information
Authors and Affiliations
Corresponding author
Additional information
Foundation item: This paper was supported by Special fund of technological innovation talents in Harbin city, China (No. 2006RFQXN003)
Electronic supplementary material is available in the online version of this article at http://dxdoi.org/10.1007/s11676-007-0013-0
Biography: Zhao Chun-Jian (1977–), male, Ph.D., research assistant in Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, P. R. China.
Rights and permissions
About this article
Cite this article
Zhao, Cj., Zu, Yg., Li, Cy. et al. Distribution of solanesol in Nicotiana tabacum . J. of For. Res. 18, 69–72 (2007). https://doi.org/10.1007/s11676-007-0013-0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11676-007-0013-0