Abstract
The second-instar healthy larvae of Clostera anastomosis were collected in the artificial woodland of poplar in Shuangcheng Town, Heilongjiang Province, China. The dead larvae of C. anastomosis infected by granulosis virus (GV) of Clostera anastomosis were grinded to obtain GV. The GV viral pesticide was diluted to seven concentrations, 1.58×103PIB·mL−1, 1.58×104 PIB·mL−1, 1.58×105 PIB·mL−1, 1.58×106 PIB·mL−1, 1.58×107 PIB·mL−1, 1.58×108 PIB·mL−1 and 1.58×109 PIB·mL−1 and the fresh poplar leaves were dipped in the seven concentrations liquids to feed the larvae. After nine days the mortality of larvae was investigated. The minimum corrected mortality (7.32%) of larvae was observed at concentrations of 1.58×103PIB·mL−1 and the maximal mortality (97.36%) was observed at concentration of 1.58×109 PIB·mL−1. The regression equation between the logarithm of the virus concentration and the mortality was y=1.946+0.558x. The LC50 was 2.97×105PIB·mL−1. The LT50 for the virus concentration of 1.58×105, 1.58×106, 1.58×107, 1.58×108, 1.58×109 PIB·mL−1 were 8.55d, 6.89d, 5.9d, 4.65d, and 4.08d, respectively, shorting gradually with the concentration increasing. It is concluded that the toxicity of Clostera anastomosis GV is very strong and as a kind of insecticides it has big potential in practical application.
Similar content being viewed by others
References
Chen Shunli, Shi Heqing, Li Youngong, Cheng Bai. 1994. Bioassay of nuclear polyhedrosis virus of the Lymantriid Parocneria Orienta Chao [J]. Journal of Fujian College of Forestry, 14(1): 58–62. (In Chinese)
Guo Yajie, Sun Xianghui, Chen Zhen, Liu Yuling. 1998. The measurement of control and occurrence of Clostera anastomosis in the forest region of Yingchun. Forestry Science & Technology, 23(1): 31–32. (In Chinese)
Jakubowska, A., van Oers, M.M., Cory, J.S. 2005. European Leucoma salicis NPV is closely related to North American Orgyia pseudotsugata MNPV [J]. Journal of Invertebrate Pathology. (88):100–107.
Jing Heming and Wang Jun. 1995. The Bioassay of Paralebeda plagifera-granulosis virus [J]. Communication of Forest Disease Insect 2: 23–24. (In Chinese)
Kolodny Hirsch D.M., Warkentin, D.L. 1993. Alvarado Rodriguez B. Spodopteraexigua Nuclear Polyhedrosis virus as a candidate viral insecticide for the beet army worm (Lepidoptera: Noctuidae) [J].Journal of Economic Entomology, 86(2): 314–321.
Li Yishuang, Wang Wenyu, Yu Zhenfu. 1989. The Experiment of bionomics and control of Clostera anastomosis [J].Forest Pest and Disease. 2: 21–27. (In Chinese)
Lin Guoxian, Huang Zhipeng, Guan Xiong. 1995. Bioassay on infectivity of Laphygma exigue Granulosis Virus [J]. Journal of Fujian Agricultural University. 24(3): 300–303. (In Chinese)
Liu Bo, Yang Jun, Jiang Chengxu, Li Bing. 1998. Bioassay of Clostera anastomosis nuclear polyhedrosis virus [J]. Jilin Forestry Science and Technology. (5): 17–18. (In Chinese)
Liu Zhenqing and Ning Xianbin. 1994. Bioassay of Stilpnotia candida Staudinger Nuclear Polyhedrosis virus. [J]. Forestry Science and Technology, (11): 29–30. (In Chinese)
Lu Zhixiao, Chen Quhou, Hong Huazhu, Yu Zehua. 1997. Bioassay of Syngrapha falcifera Nuclear Polyhedrosis virus [J]. Chinese Journal of Biological Control, 13(4): 151–153. (In Chinese)
Martins, T., Montiel, R., Medeiros, J., Oliveir, L. 2005. Occurrence and characterization of a nucleopolyhedrovirus from Spodoptera littoralis (Lepidoptera: Noctuidae) isolated in the azores. Journal of Invertebrate Pathology, (89): 185–192
Wang Fuwei, Niu Yanzhang, Hou Liwei, Pi Zhongqing, Wang Jinghui, Chen Yuheng. 1998. Study on the Bionomics and Control of Clostera anastomosis [J].Forest Research, 11(3): 32–329. (in Chinese)
Wang Zhiying, Yue Hua, Zhang Guocai, Li Suqiong. 1994. Bioassy of ZrGV on the Zethenia rufescentaria Motsch. [J]. Journal of Northeast Forestry University, 22(6): 14–17: (In Chinese)
Yang Zhende, Zhu Lin, Zhao Boguang, Fang Jie. 2003. Observation of biological characteristics of Clostera anastomosis L. Massin Laboratory [J]. Journal of Guangxi Academy of Sciences. 20(1): 35–37. (In Chinese)
Zhang Dongfang, Chen Jinxiu, Xu Qingfeng. 2001. Bioassay of Heliothis assaultaNuclear Polyhedrosis virus [J]. Journal of Anhui Agricultural Sciences, 7(3): 31–33. (In Chinese)
Zhou Jianhua, Zhao Rong, Guo Hengxiao. 1999. A biological determination of LdGV’s Poison [J]. Journal of Sichuan Forestry Science and Technology. 20(3): 15–17. (In Chinese)
Zu Aimin, Dai Meixue. 1997. The Bioassay and field evaluation of the nuclear polyhedrosis virus from Orgyia Ericae [J].Chinese Journal of Biological Control, 13(2): 57–60. (In Chinese)
Author information
Authors and Affiliations
Additional information
Biography: LI Hai-xia (1980–), female, Master degree student in Heilongjiang Forest Research Institute, Harping Rd., Harbin, 150040, P. R. China.
Rights and permissions
About this article
Cite this article
Li, Hx., Wang, Zy., Guo, Sp. et al. Bioassay of Clostera anastomosis granulosis virus. J. of For. Res. 17, 50–52 (2006). https://doi.org/10.1007/s11676-006-0012-6
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11676-006-0012-6