Microwave Synthesis of Nearly Monodisperse Core/Multishell Quantum Dots with Cell Imaging Applications
We report in this article the microwave synthesis of relatively monodisperse, highly crystalline CdSe quantum dots (QDs) overcoated with Cd0.5Zn0.5S/ZnS multishells. The as-prepared QDs exhibited narrow photoluminescence bandwidth as the consequence of homogeneous size distribution and uniform crystallinity, which was confirmed by transmission electron microscopy. A high photoluminescence quantum yield up to 80% was measured for the core/multishell nanocrystals. Finally, the resulting CdSe/Cd0.5Zn0.5S/ZnS core/multishell QDs have been successfully applied to the labeling and imaging of breast cancer cells (SK-BR3).
KeywordsMicrowave Semiconductor Quantum Dots Photoluminescence Cell labeling
The potential biological applications of colloidal semiconductor nanocrystals have been gaining more and more interest, since they were first reported by Alivisatos and Nie groups [1, 2]. Compared with traditional organic dyes, semiconductor quantum dots (QDs) have exceptional intrinsic properties including high photoluminescence quantum yield, narrow fluorescence emission, long fluorescence lifetime, large effective Stokes shifts, and tunable excitation wavelength, making them very appealing for various biological applications [3, 4, 5]. Therefore, the development of simple, low-cost, and scalable synthetic methods for the mass production of monodisperse, stable, and size/shape-controlled nanocrystals will be very important.
Efforts have been devoted to the preparation, surface functionalization, and various biological applications of colloidal nanocrystals over the past two decades. Various synthetic approaches have been explored to produce colloidal nanocrystals with uniform size and shape [6, 7, 8]. The so-called hot injection method, which involves injecting a reactant precursor solution into a reactor containing a mixture of other reactants and surfactants at high temperature, has been one of the most successful and popular approaches, since the synthesis of high-quality semiconductor QDs was first reported . The fast introduction of precursor solution initiates a short burst of homogeneous nucleation that is stopped with a sudden decrease in temperature to prevent excess nucleation which is favorable for the succeeding growth of the initially formed nuclei. Thus, monodisperse, highly crystalline nanocrystals are usually prepared using this method, and in some cases, with some evolutional improvements [9, 10].
The “hot injection” method, however, utilizes conventional convective heating that can lead to nonuniform reaction conditions caused by sharp thermal gradients in the bulk solution. Bulk scale reactions are more affected by this heating process because of the large volume and heat stability during injections. Enormous effort has been made to overcome these problems [8, 11, 12, 13]. One method found to be effective and convenient is by using microwave heating during synthesis. Irradiation with a microwave instead of convective heating overcomes heterogeneity of the heat distribution in the solution. Gerbec and co-workers studied microwave heating method and successfully prepared InGaP, InP, and CdSe nanoparticles . Roy et al. reported microwave synthesis of the core CdSe QDs and coated them with ZnS . However, the nanocrystals prepared with their methods were characterized with large full-width-at-half-maximum (FWHM) bandwidths (36–70 nm). Moreover, in many cases [15, 16], the crystallinity and optical properties of the nanoparticles appeared to be of low grade.
By overcoating with layers of shell to establish a core/shell/shell system, such as CdSe/CdS/ZnS [17, 18] and CdSe/ZnSe/ZnS [19, 20], nanocrystals have been shown to be generally more robust and of higher photoluminescence quantum yield (PLQY). In this paper, we present the synthesis of relatively monodisperse, highly crystalline CdSe QDs using microwave heating followed by the conventional shell-growth method to produce CdSe/Cd0.5Zn0.5S/ZnS core/multishell QDs. The microwave-synthesized CdSe nanocrystal cores exhibited high crystallinity and narrow size distribution (FWHM, ~25 nm). Moreover, the resulting CdSe/Cd0.5Zn0.5S/ZnS core/multishell QDs were measured with a high PLQY up to 80%. The core/multishell QDs were successfully applied to the labeling and imaging of cancer cells, exhibiting great promise for biological applications.
Cadmium oxide (99.99%), zinc oxide (99.9%), selenium (99.999%, 200 mesh), sulfur (99.999%, powder), tributylphosphine (TBP, 93%), and n-octadecylphosphonic acid (ODPA, 98%) were purchased from Alfa Aesar. Trioctylphosphine oxide (TOPO, 90%), 1-octadecene (ODE), oleic acid (OA, 90%), octadecylamine (ODA, 97%), n-(3-dimethylaminopropyl)-n’-ethylcarbodiimide hydrochloride (EDC), n-hydroxysulfosuccinimide sodium salt (sulfo-NHS) and Dulbecco’s phosphate-buffered saline (DPBS) were obtained from Sigma–Aldrich. All organic solvents were purchased from EM Sciences. All chemicals were used directly without any further purification.
Preparation of CdSe Core
For the synthesis of CdSe cores, ODA, and TOPO were used as the ligands. Cadmium precursor was prepared by conventional heating of a mixture of 0.24 mmol of CdO, 0.51 mmol of ODPA, and 9.8 g of ODE to 300°C for 15 min to form an optically clear solution. Selenium precursor was prepared by dissolving 0.173 g of Se powder in 0.893 g of TBP in nitrogen environment. Both precursors were used in the microwave nanoparticle synthesis that was carried out in a H2800 microwave processor (Energy Beam Sciences, Inc., USA) equipped with a thermometer.
For the microwave synthesis of CdSe QDs, 1.1 ml of the Se precursor solution was injected into a 100-ml sealed flask containing 10 g of Cd precursor, 9 g of ODA, and 1.5 g of TOPO, which had been degassed in nitrogen. This mixture was microwaved to 50°C for 15 min to melt all the solids into a homogeneous solution. The solution was microwave heated up to 200°C at the maximum power (800 W) after which the microwave power was set at 400 W in order to keep the reaction temperature constant for a period of time to reach the desired size. Once the corresponding QD size was attained, the microwave was turned off, and the mixture was allowed to cool down to room temperature before being taken out of the microwave oven. Hexane was added to disperse the CdSe QDs, which were then purified by repeated acetone precipitation.
Preparation of CdSe/Cd0.5Zn0.5/ZnS Core/Multishell QDs
CdSe/Cd0.5Zn0.5/ZnS core/multishell QDs were synthesized under conventional convective heating in order to apply the successive ion layer adsorption and reaction (SILAR) method  with slight modification. Less toxic reactant precursors, including metal oxide and elemental sulfur, were used to grow shells. The precursor solutions were prepared as reported previously  with the necessary doses for each monolayer growth calculated from the particle size and concentration according to the method developed by Peng et al. .
A typical synthesis was performed as follows: 1.3 × 10−6 mol of purified CdSe core QDs dissolved in 30 g of ODE, and 10.0 g of ODA was loaded into a 100-ml flask and heated on a heating mantle to 120°C for 15 min under nitrogen. The mixture was heated to 210°C to grow the shells at 15 min for each injection. The amounts of shell precursor injection solutions for the first two layers of Cd0.5Zn0.5S and the third layer of ZnS were 0.74, 1.6, and 2.9 ml, respectively. Finally, the solution was cooled to 150°C for 40 min and then allowed to reach room temperature. The core/multishell QDs were purified several times by extracting with hexane and precipitating with acetone.
Bioconjutation of Core/Multishell QDs
Surface modification of the purified QDs was performed using a previously reported method . This method converts the QDs into water-soluble and biocompatible nanocrystals.
The water-soluble QDs were conjugated with goat-anti-mouse antibody (Rockland, USA) using N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) as the coupling agent. Briefly, 1 mg of N-hydroxysulfosuccinimide sodium salt (sulfo-NHS) and 2 mg of EDC were mixed with 1 ml QDs water solution (100 nM). To this was added a 0.5 mg of goat-anti-mouse antibody dissolved in deionized water with vigorous stirring for 2.0 h at room temperature. The antibody-conjugated QDs were purified by dialyzing in 50 mM borate buffer.
Cell Imaging of Core/Multishell QDs
The secondary antibody (goat anti-mouse antibody)-conjugated CdSe/Cd0.5Zn0.5/ZnS QDs was used to create fluorescent images of breast cancer cells, SK-BR3. To carry this process out, 0.1 ml of SK-BR3 suspension at a cell concentration of 1 × 106 cells/ml in DPBS was transferred into a sterile Eppendorf tube. A 1-μg sample of anti-EpCAM (Epithelial cell adhesion molecule) was added to the SK-BR3 cells and incubated for 30 min at 37°C. The cells were washed 3 times with 1 ml DPBS and precipitated at 3000 rpm for 5 min. The pelleted cells were resuspended in 50 μl DPBS. The anti-EpCAM-labeled cells were exposed to 50 pmol of QDs-goat-anti-mouse antibody conjugates and incubated for another 30 min at 37°C. The cells were washed three times with 1 ml Ocean blocking buffer B (Ocean Nanotech LLC, USA) to remove the non-attached QDs-goat-anti-mouse antibody conjugates. The washed cells were finally resuspended in 20 μl DPBS and imaged.
UV–vis absorption spectra were collected with an HP 8453 UV/vis spectrometer. Photoluminescence (PL) and photoluminescence excitation (PLE) spectra were measured on a Perkin Elmer Lambda LS 50B luminescence spectrometer. PL spectra were recorded at an excitation wavelength of 400 nm, and excitation spectra were measured at the wavelength of the fluorescence maximum . PL quantum yields were determined using a conventional method by comparison of the integrated fluorescence intensity with that of standard dye solutions. X-ray diffraction (XRD) patterns were obtained using a Rigaku D/max-gB diffractometer. Transmission electron microscopy (TEM) and high-resolution (HR) TEM images were recorded on a JEOL 100CX electron microscope operated at 80 kV and a Philips CM 200 electron microscope operated at 200 kV, respectively. For cell imaging, the QD-labeled cells were dropped onto a hemocytometer and observed under an Emscope fluorescence microscope.
Results and Discussion
Accompanied by the long wavelength shift of PL was a large increase in PLQY. The PLQY of the CdSe QDs was found to be 30%, which increased to above 80% for CdSe/Cd0.5Zn0.5S/ZnS core/multishell QDs. The PL emission of the core/multishell QDs was clearly visible to the naked eye under white light and a UV lamp. This again shows that the microwave-synthesized CdSe cores were of good quality which is essential for the preparation of highly fluorescent core/shell QD materials. In addition, large Stokes shifts can be observed for both the CdSe cores and CdSe/Cd0.5Zn0.5S/ZnS core/multishell QDs (Fig. 3). The PLE spectra of the microwave-synthesized QDs were also shown in Fig. 3 (dotted line). The spectra were normalized to the intensity of the first exciton absorption peak, which allowed a direct comparison between PLE and absorption. For CdSe nanocrystal cores, the PLE spectrum and absorption spectrum were highly consistent and exhibited similar excitonic features, which further verified the high-quality of microwave-synthesized CdSe crystal cores. After the growth of shells, the PLE spectrum deviated slightly from the corresponding absorption spectrum, especially at the short wavelength side of the first-order excitonic feature; this can be explained by the different shell contributions to PLE and absorption . However, the PLE spectra still largely resembles the features of the corresponding absorption spectra, again, confirming the good quality of the microwave-synthesized CdSe cores and core/shell QDs.
In summary, we have prepared relatively monodisperse CdSe QDs through the microwave heating method. The prepared CdSe nanocrystal cores exhibited narrow band photoluminescence with homogeneous size distributions and uniform crystallinity under TEM and HRTEM. The HRTEM images supported the superior quality of the microwave-synthesized CdSe/Cd0.5Zn0.5S/ZnS core/multishell QDs, which was further confirmed with the high PLQY up to 80%. Finally, the QDs were successfully employed to label and image breast cancer cells (SK-BR3) showing promise for use in biological studies.
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