Abstract
Objective
To evaluate the effects of HPV16 E6/E7 siRNAs on cervical cancer SiHa cells.
Methods
The expressions of the E6, E7, p53 and Rb genes were assayed by RT-PCR and Western-bloting respectively. The proliferation and apoptosis of the cells were evaluated by MTT and flow cytometry.
Results
HPV 16 E6 and E7 oncogenes were selectivly downregulated by HPV 16 E6 and E7 siRNAs, which sustained at least 96 h by single dose siRNA. Furthermore, reduction of E6 and E7 oncogenes expression upregulated the expressions of P53 and RB protein and induced apoptosis in SiHa cells.
Conclusion
Introduction of HPV16 E6/E7 siRNA might be a potentially potent and specific approach to inhibit proliferation and induce apoptosis of SiHa cervical cancer cells.
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This work was supported by the grants from the National Natural Science Foundation of China(No. 30660192) and the Key Project of Education Committee of Jiangxi Province (No. 2005-179).
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Nie, Cl., Gao, Gl., Han, J. et al. Human papillomavirus 16 E6, E7 siRNAs inhibit proliferation and induce apoptosis of SiHa cervical cancer cells. Chin. J. Cancer Res. 20, 301–306 (2008). https://doi.org/10.1007/s11670-008-0301-z
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DOI: https://doi.org/10.1007/s11670-008-0301-z