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Early diagnosis of epithelial ovarian cancer with cDNA microarry

  • Published:
Chinese Journal of Cancer Research

Abstract

Objective

To investigate the roles of tumor suppressor genes-PTEN, nm23H1, VEGF165, Tiam1, MMP-2, Timp2, HE4 and S100A4 in tumorigenesis and progression of epithelial ovarian cancer(EOC) and develop a novel method for the early diagnosis of EOC.

Methods

We observed the different expression profiles of those genes in normal ovary(n=5) and ovarian cancer tissue (n=20) by cDNA microarray.

Results

In EOC, PTEN, Timp2 and nm23H1 genes were down-regulated (CY-3/CY-5<0.5), compared with the normal ovary, whereas Tiam1, VEGF165, MMP-2, HE4 and S100A4 genes were up-regulated (CY-3/CY-5>2.0). Among them, HE4 gene was remarkably elevated (CY-3/CY-5>5.0). Their expression was correlated with clinicopathological staging of EOC(P<0.01).

Conclusion

Those genes are closely linked to the pathogenesis and progression of EOC. cDNA microarray is an effective technique to screen molecular biological markers and predict the metastastic potential of EOC in early diagnosis.

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Authors and Affiliations

  1. The Fourth Affiliated Hospital, China medical University, Shengyang, 110032, China

    Ying Chen  (陈颖)

  2. Department of Biochemistry and Molecular Biology, College of Basic Medicine, Shengyang, China

    Hua-chuan Zheng  (郑华川)

  3. Center of Biochip, Shengyang, China

    Yu-jie Zhao  (赵雨杰)

  4. Cancer Institute, The First Affiliated Hospital, China Medical University, Shenyang, 110001, China

    Yan Xin  (辛彦)

Authors
  1. Ying Chen  (陈颖)
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  2. Hua-chuan Zheng  (郑华川)
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  3. Yu-jie Zhao  (赵雨杰)
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  4. Yan Xin  (辛彦)
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Corresponding author

Correspondence to Ying Chen  (陈颖).

Additional information

This work was supported by the Scientific Research Foundation from Education Office of Liaoning Provice(No. 20060961) and Science Technology Project of Shenyang (No. 1063230-3-00).

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Chen, Y., Zheng, Hc., Zhao, Yj. et al. Early diagnosis of epithelial ovarian cancer with cDNA microarry. Chin. J. Cancer Res. 20, 110–114 (2008). https://doi.org/10.1007/s11670-008-0110-4

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  • DOI: https://doi.org/10.1007/s11670-008-0110-4

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