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Helicobacter pylori infection in association with cell proliferation, apoptosis and prostaglandin E2 levels

  • Gastrointestinal Cancer
  • Published:
Chinese Journal of Cancer Research

Abstract

Objective

To evaluate the relationship between H. pylori infection with cell proliferation, apoptosis and PGE2 levels.

Methods

A population-based study was conducted in Linqu, a high-risk area of gastric cancer in China. A total of 1523 subjects, aged 35–64, participating in a gastric cancer screening survey were investigated. H. pylori status were determined by 13C-urea breath test, expressions of Ki-67 were assessed by immunohistochemistry, apoptotic cells were detected by terminal deoxynucleotide transferase mediated dUTP nick end-labeling (TUNEL) method, and PGE2 levels were measured by enzyme immunoassay.

Results

H. pylori infection was positively associated with cell proliferation activity. The mean and median percentage of Ki-67 labeling index (LI) in subjects with H. pylori positive were 14.1±10.3 and 12.0, significantly higher than those with H. pylori negative (\(\overline x \)±s: 8.4±7.0; median: 5.8; P<0.0001). Moreover, the prevalence rates of H. pylori infection showed a tendency to increase according to severity score of cell apoptosis (Ptrend <0.0001), from score 0 to 3, the percentage of H. pylori positivity increased from 67.5% to 96.7%. Furthermore, The mean and median of PGE2 concentration were 628.84±726.40 pg/mL and 411.33 pg/mL among subjects with H. pylori positive compared with 658.19±575.91pg/mL and 455.97 pg/mL among those with H. pylori negative (P=0.209).

Conclusion

H. pylori infection was positively associated with increased cell proliferation and apoptosis activity, suggesting that H. pylori infection plays an important role in the gastric epithelial cell malignant transformation.

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Authors and Affiliations

  1. Department of Cancer Epidemiology, Beijing University School of Oncology, Beijing Institute for Cancer Research, Beijing Cancer Hospital, Beijing, 100036, China

    Pan Kai-feng  (潘凯枫), Zhang Yang  (张阳), Zhang Lian  (张联), Ma Jun-ling  (马峻岭), Feng Guo-shuang  (冯国双), Zhou Tong  (周彤) & You Wei-cheng  (游伟程)

Authors
  1. Pan Kai-feng  (潘凯枫)
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  2. Zhang Yang  (张阳)
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  3. Zhang Lian  (张联)
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  4. Ma Jun-ling  (马峻岭)
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  5. Feng Guo-shuang  (冯国双)
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  6. Zhou Tong  (周彤)
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  7. You Wei-cheng  (游伟程)
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Corresponding author

Correspondence to You Wei-cheng  (游伟程).

Additional information

This work was supported by the Grants from National “211” Project in Peking University (Grant 529 and 533), Beijing Municipal Commission of Science and Technology Grant (H0209-20030130).

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Pan, Kf., Zhang, Y., Zhang, L. et al. Helicobacter pylori infection in association with cell proliferation, apoptosis and prostaglandin E2 levels. Chin. J. Cancer Res. 19, 249–254 (2007). https://doi.org/10.1007/s11670-007-0249-4

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  • DOI: https://doi.org/10.1007/s11670-007-0249-4

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