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Effects of triptolide on cell proliferation and CXCR4 expression in Burkitt’s lymphoma Raji cells in vitro

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Abstract

Objective

To investigate the inhibitory effects of triptolide on cell proliferation and CXCR4 expression in Burkitt’s lymphoma cell line Raji cells.

Methods

The effects of triptolide on the growth of Raji cells were studied by 3-(4, 5-Dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium(MTT) assay. The effects of triptolide on CXCR4 expression on Raji cells were studied by flow cytometric analysis. Chemotaxis assays were performed to observe the effects of triptolide on migration of Raji cells towards recombinant human SDF-1α (rhSDF-1α) in vitro.

Results

Triptolide inhibited the proliferation of Raji cells in a dose-and time-dependent way with a 24-h IC50 value of 43.06 nmol/L and a 36-h IC50 value of 25.08 nmol/L. Triptolide could downregulate the CXCR4 expression on Raji cells in a dose-dependent manner. Furthermore, chemotaxis assays showed that triptolide could block the migration of Raji cells to rhSDF-1α in vitro, and the inhibition was dose-dependent.

Conclusion

Triptolide could inhibit the proliferation and migration of Raji cells in vitro. The underlying anti-tumor mechanism of triptolide might be related to the anti-proliferative effect and the blockage of SDF-1/CXCR4 axis.

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Author information

Correspondence to Yan Chen 陈燕.

Additional information

This project was supported by the National Natural Science Foundation of China (No. 30472267).

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Zhang, C., Cui, G., Liu, F. et al. Effects of triptolide on cell proliferation and CXCR4 expression in Burkitt’s lymphoma Raji cells in vitro . Chin. J. Cancer Res. 19, 27–31 (2007). https://doi.org/10.1007/s11670-007-0027-3

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CLC number

  • R73-36

Key words

  • Triptolide
  • Raji cells
  • Proliferation
  • CXCR4