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Effects of triptolide on cell proliferation and CXCR4 expression in Burkitt’s lymphoma Raji cells in vitro

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To investigate the inhibitory effects of triptolide on cell proliferation and CXCR4 expression in Burkitt’s lymphoma cell line Raji cells.


The effects of triptolide on the growth of Raji cells were studied by 3-(4, 5-Dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium(MTT) assay. The effects of triptolide on CXCR4 expression on Raji cells were studied by flow cytometric analysis. Chemotaxis assays were performed to observe the effects of triptolide on migration of Raji cells towards recombinant human SDF-1α (rhSDF-1α) in vitro.


Triptolide inhibited the proliferation of Raji cells in a dose-and time-dependent way with a 24-h IC50 value of 43.06 nmol/L and a 36-h IC50 value of 25.08 nmol/L. Triptolide could downregulate the CXCR4 expression on Raji cells in a dose-dependent manner. Furthermore, chemotaxis assays showed that triptolide could block the migration of Raji cells to rhSDF-1α in vitro, and the inhibition was dose-dependent.


Triptolide could inhibit the proliferation and migration of Raji cells in vitro. The underlying anti-tumor mechanism of triptolide might be related to the anti-proliferative effect and the blockage of SDF-1/CXCR4 axis.

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  1. [1]

    Chen BJ. Triptolide, a novel immunosuppressive and anti-inflammatory agent purified from a Chinese herb Tripterygium wilfordii Hook F [J]. Leuk Lymphoma 2001; 42:253–65.

  2. [2]

    Yang S, Chen J, Guo Z, et al. Triptolide inhibits the growth and metastasis of solid tumors [J]. Mol Cancer Ther 2003; 2:65–72.

  3. [3]

    Chang WT, Kang JJ, Lee KY, et al. Triptolide and chemotherapy cooperate in tumor cell apoptosis. A role for the p53 pathway [J]. J Biol Chem 2001; 276:2221–7.

  4. [4]

    Yinjun L, Jie J, Yungui W. Triptolide inhibits transcription factor NF-kappaB and induces apoptosis of multiple myeloma cells [J]. Leuk Res 2005; 29:99–105.

  5. [5]

    Choi YJ, Kim TG, Kim YH, et al. Immunosuppressant PG490 (triptolide) induces apoptosis through the activation of caspase-3 and down-regulation of XIAP in U937 cells [J]. Biochem Pharmacol 2003; 66:273–80.

  6. [6]

    Sanchez-Madrid F, del Pozo MA. Leukocyte polarization in cell migration and immune reactions [J]. EMBO J 1999; 18:501–11.

  7. [7]

    Kucia M, Reca R, Miekus K, et al. Trafficking of normal stem cells and metastasis of cancer stem cells involve similar mechanisms: pivotal role of the SDF-1-CXCR4 axis [J]. Stem Cells 2005; 23:879–94.

  8. [8]

    Muller A, Homey B, Soto H, et al. Involvement of chemokine receptors in breast cancer metastasis [J]. Nature 2001; 410:50–6.

  9. [9]

    Phillips RJ, Burdick MD, Lutz M, et al. The Stromal derived factor-1/CXCL 12-CXC chemokine receptor 4 biological axis in non-small cell lung cancer metastases [J]. Am J Respir Crit Care Med 2003; 167:1676–86.

  10. [10]

    Bertolini F, Dell’Agnola C, Mancuso P, et al. CXCR4 neutralization, a novel therapeutic approach for non-Hodgkin’s lymphoma [J]. Cancer Res 2002; 62:3106–12.

  11. [11]

    Trentin L, Cabrelle A, Facco M, et al. Homeostatic chemokines drive migration of malignant B cells in patients with non-Hodgkin lymphomas [J]. Blood 2004; 104:502–8.

  12. [12]

    Bradstock KF, Makrynikola V, Bianchi A, et al. Effects of the chemokine Stromal cell-derived factor-1 on the migration and localization of precursor-B acute lymphoblastic leukemia cells within bone marrow Stromal layers [J]. Leukemia 2000; 14:882–8.

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Author information

Correspondence to Yan Chen 陈燕.

Additional information

This project was supported by the National Natural Science Foundation of China (No. 30472267).

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Zhang, C., Cui, G., Liu, F. et al. Effects of triptolide on cell proliferation and CXCR4 expression in Burkitt’s lymphoma Raji cells in vitro . Chin. J. Cancer Res. 19, 27–31 (2007).

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CLC number

  • R73-36

Key words

  • Triptolide
  • Raji cells
  • Proliferation
  • CXCR4