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Detection of SBEM-mRNA in peripheral blood of patients with breast cancer and its clinical significance

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Chinese Journal of Cancer Research

Abstract

Objective

The aim of the present study is to explore the expression of a specific marker of breast cancer, small breast epithelial mucin (SBEM) mRNA, in peripheral blood and to investigate its significance.

Methods

The expressions of SBEM-mRNA in peripheral blood of 67 patients with breast cancer, 16 patients with benign breast disease, and 20 normal healthy volunteers were detected with nested reverse transcription-polymerase chain reaction (Nested-RT-PCR).

Results

SBEM-mRNA was negative in healthy individuals and patients with benign breast tumor, but 50.7%(34/67) of breast cancer patients showed positive expression of SBEM-mRNA in peripheral blood, of whom 25%(2/8) were in stage I, 45.8%(11/24) in stage II, 43.75%(11/24) in stage III and 73.7(14/19) in stage IV. The positive rate in stage IV was higher than that in stage I, II, III (P<0.05). Expressions of SBEM-mRNA had no correlation with age, carcinoma size, pathological type, ER and PR state (P<0.05).

Conclusion

SEBM-mRNA is specifically expressed in breast cancer and it may act as a marker for the detection of micrometastasis of breast cancer.

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Authors and Affiliations

  1. Department of Gastroenteritis & Breast, Guangxi Medical University, Nanning, 530021, China

    Yang Hua-Wei  (杨华伟), Yang Nan-Wu  (杨南武), Zhang Chuan-Min  (张传珉), Chen Jian-Si  (陈建思) & Jiang Yi  (蒋奕)

  2. Department of Pathology, Affiliated Cancer Hospital, Guangxi Medical University, Nanning, 530021, China

    Cao Ji  (曹骥), Ou Chao  (欧超) & Su Jian-Jia  (苏建家)

Authors
  1. Yang Hua-Wei  (杨华伟)
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  2. Yang Nan-Wu  (杨南武)
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  3. Cao Ji  (曹骥)
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  4. Liu Jian-Lun  (刘剑仑)
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  5. Zhang Chuan-Min  (张传珉)
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  6. Chen Jian-Si  (陈建思)
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  7. Jiang Yi  (蒋奕)
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  8. Ou Chao  (欧超)
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  9. Su Jian-Jia  (苏建家)
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Corresponding author

Correspondence to Yang Nan-Wu  (杨南武).

Additional information

Foundation item: This work was supported by the Natural Science Foundation of Guanxi Province (No. 0640161).

Biography: YANG Hua-wei (1973–), male, master of medicine, Guangxi Medical University, majors in surgical oncology.

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Yang, HW., Yang, NW., Cao, J. et al. Detection of SBEM-mRNA in peripheral blood of patients with breast cancer and its clinical significance. Chin. J. Cancer Res. 18, 294–298 (2006). https://doi.org/10.1007/s11670-006-0294-4

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  • DOI: https://doi.org/10.1007/s11670-006-0294-4

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