Abstract
Objective
This paper attempts to discuss the effects of surviving antisense RNA on doxorubicin-induced apoptosis in pancreatic cancer cell line PANC-1.
Methods
A surviving antisense eukaryotic vector pcDNA3-SV Vas prepared in previous study was delivered into PANC-1 by electroperforation. Cell survival fraction and MTT assay were used to investigate the sensibility of transfected cells to doxorubicin. Apoptosis was detected by DNA gel electrophoresis.
Results
We obtained two positive cell clone PANC—1/SVVas and PANC-1/neo cells, the growth of PANC-1/SV Vas cells was significantly reduced (P<0.05). By MTT assay, the IC50 to doxorubicin of PANC-1/SV Vas, PANC-1/neo and PANC-1 cells were (0.285±0.012) μ mol/L, (1.528±0.317) μ mol/L and (1.540±0.253) μ mol/L respectively, the difference was significant by statistic analysis (P<0.01). Agarose gel electrophoresis of genomic DNA from PANC/SV Vas showed typical DNA ladder, but DNA from PANC-1/neo and PANC-1 did not.
Conclusion
Survivin antisense RNA could enhance doxorubicin-induced apoptosis in pancreatic cancer cell line PANC-1. This may lay an experimental foundation for further research of gene therapy in pancreatic cancer.
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Foundation item: This work was supported by a grant from the Natural Sciences Foundation of the Inner Mongolia Autonomous Region (No. 200308020605).
Biography: SHEN Jing-hua(1960–), female, master of medicine, associate professor, Inner Mongolia Medical College, majors in medical oncology.
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Shen, Jh., Wang, Xj., Wang, Xm. et al. Antisense RNA of survivin enhances the sensitivity of pancreatic cancer cell line PANC-1 to doxorubicin. Chin. J. Cancer Res. 18, 138–141 (2006). https://doi.org/10.1007/s11670-006-0138-2
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DOI: https://doi.org/10.1007/s11670-006-0138-2