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Establishment of intraperitoneal transplantation model of cisplatin-resistant ovarian carcinoma cell in scid mice

  • Published:
Chinese Journal of Cancer Research

Abstract

Objective

to develop an intraperitoneal transplantation model of human ovarian carcinoma SKOV3/CDDP cell in severe combined immunodeficiency (SCID) mouse and to study its biologic characteristics.

Methods

Sixteen qualified C.B17/SCID mouse were divided into two groups randomly. Human ovarian carcinoma SKOV3 or SKOV3/CDDP cells were injected intraperitoneally into the SCID mouse at the amount of 1×107 cells (0.5 mL) per mouse. The behaviors of mice, tumor growth and morphology were analyzed. The expression of cancer antigen 125 (CA 125), GST-π and Topo-II were examined by immunohistochemical method.

Results

In this experimental study, transplanted tumors are formed in 100% SCID mice in the two groups. The morphology, growth pattern and CA125 secretion of SKOV3/CDDP group were as same as those of SKOV3 group. It shows that the tumors of the two groups kept the characteristics of ovaries serosity papillary adenocarcinoma. Compared with SKOV3 group, the expression of GST-π and Topo-II gene in SKOV3/CDDP group were significantly higher (P<0.05).

Conclusion

An intraperitoneal transplantation model of human ovarian carcinoma SKOV3/CDDP in SCID mice has been developed successfully. It may be an ideal animal model for biotherapy research of ovarian carcinoma as it can simulate the biological behavior of peritoneal metastasis of human ovarian carcinoma and the drug tolerance is maintained.

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Authors and Affiliations

  1. Department of Gynaecology & Obstetrics, Hebei Medical University, Shijiazhuang, 050011, China

    Zhang Hui  (张辉), Jia Jin-hua  (贾金华) & Kang Shan  (康山)

  2. Department of Surgery, Hebei Medical University, Shijiazhuang, 050011, China

    Zhao Qun  (赵群)

  3. Department of Pathology, Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuang, 050011, China

    Wang Xiao-ling  (王晓玲) & Wang Yong-jun  (王永军)

  4. Institute of Cancer, Hebei Medical University, Shijiazhuang, 050011, China

    Zuo Lian-fu  (左连富)

Authors
  1. Zhang Hui  (张辉)
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  2. Zhao Qun  (赵群)
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  3. Zuo Lian-fu  (左连富)
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  4. Wang Xiao-ling  (王晓玲)
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  5. Wang Yong-jun  (王永军)
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  6. Jia Jin-hua  (贾金华)
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  7. Kang Shan  (康山)
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Corresponding author

Correspondence to Zhao Qun  (赵群).

Additional information

Foundation item: This work was supported by a grant from the Science & Technology Research Project, Public Health Department of Hebei Province (No. 2003/03086)

Biography: ZHANG Hui(1969–), female, doctor of medicine, associate professor, Fourth Affiliated Hospital, Hebei Medical University, majors in Gynaecological Oncology.

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Zhang, H., Zhao, Q., Zuo, Lf. et al. Establishment of intraperitoneal transplantation model of cisplatin-resistant ovarian carcinoma cell in scid mice. Chin. J. Cancer Res. 18, 127–131 (2006). https://doi.org/10.1007/s11670-006-0127-5

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  • DOI: https://doi.org/10.1007/s11670-006-0127-5

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