Abstract
Objective: To investigate the relationship between the expression of p73α and p53 versus the chemosensitivity in breast cancer cells. Methods: Twelve surgical samples of breast cancer diagnosed by pathology were used. The cancer sample cells were separately cultured in the incubator at 37°C, 5% CO2 in vitro. The relative inhibition rate of cancer cells by 4 kinds of anticancer drugs, which were EPI, MMC, 5-Fu and DDP, were assayed by MTT method. Immunocytochemistry was used to detect the expression of p73α and p53 in the cancer cells. Results: The positive expression of p73α was found in 5/12 (41.67%), and p53 positive expression rate was 50.0% (6/12). The relative inhibition rate of MMC, EPI, 5-Fu and DDP were significantly higher in the p73α positive cancer cells than in the p73α negative cancer cells. A positive correlation was found between expression of p73α and chemosensitivity for all the four anticancer drugs. Conclusion: The expression of p73α is related with the chemosensitivity of the breast cancer cells, and it may become one of the markers for judging the effect of chemotherapy in clinic.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Kaghad M, Bonnet H, Yang A, et al. Monoallelically expressed gene related to p53 at lp36, a region frequently deleted in neuroblastoma and human cancers [J]. Cell 1997; 90:809–19.
Weber A, Bellmann U, Bootz F, et al. Expression of p53 and its homologues in primary and recurrent squamous cell carcinomas of the head and neck [J]. Int J Cancer 2002; 99:22–8.
Gong JG, Costanzo A, Yang HQ, et al. The tyrosine kinase c-Abl regulates p73 in apoptotic response to cisplatin-induced DNA damage [J]. Nature 1999; 399:806–9.
Zhu J, Nozell S, Wang J, et al. p73 cooperates with DNA damage agents to induce apoptosis in MCF7 cells in a p53-dependent manner [J]. Oncogene 2000; 20:4050–7.
He Y, Fan SZ, Jiang YG, et al. Effect of p73 gene on chemosensitivity of human lung adenocarcinoma cells H1299 [J]. Chin J Cancer 2004; 236:645–9.
Author information
Authors and Affiliations
Corresponding author
Additional information
Biography: ZHOU Xin (1975–), male, master of medicine, physician, Chongqing University of Medical Sciences, majors in research on breast cancer.
Rights and permissions
About this article
Cite this article
Zhou, X., Sun, Zj. Study on relationship between chemosensitivity of breast cancer and expression of p73α and p53. Chin. J. Cancer Res. 17, 224–226 (2005). https://doi.org/10.1007/s11670-005-0045-y
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11670-005-0045-y