Abstracts
Objective: To investigate the effect of nicotine on growth and apoptosis-related gene expression of human small lung cancer cell. Methods: NCI-H446 cell line was cultured in the presence of various concentrations (1∼1000 ng/ml) of nicotine for 48 h. MTT was applied to evaluate the effect of nicotine on the growth of NCL-H446, small cell lung carcinoma (SCLC) cell line. After NCI-H446 cells were treated with 100 ng/ml for 48 h, human apoptosis-related gene cDNA expression profile microarray was used to detect the expression of 451 apoptosis-related genes in NCL-H446 cell line. Results: Significant proliferation promotion of nicotine (1∼10 ng/ml) on NCI-LH446 cells were observed, but with decreased promotion effect with increased concentration of nicotine in culture. Growth inhibition rates for 1, 10, 100 and 1000 ng/ml nicotine were −91.0%, −41.8%, −40.0% and 27.3% respectively. Microarray detection showed that significantly different expressions were found in71 of 451 apoptosis-related genes. 38 apoptosis-promoting gene and 30 apoptosis-inhibiting genes were up-regulated significantly (cy3/cy5>2.0), and only 3 showed significant down-regulation (cy3/cy5<0.5). Conclusion: Nicotine may promote growth of human SCLC cell, and regulate expression of apoptosis-related genes.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Lawrence D. Nicotine may promote tumor growth, us researchers suggest [J]. Lancet 2003; 361:146.
Shimoyama Y, Kubota T, Watanabe M, et al. Predictability of in vivo chemosensitivity by in vitro MTT assay with reference to the clonogenic assay [J]. J Surg Oncol 1989; 41:12–8.
Novak J, Escobedo-Morse A, Kelley K, et al. Nicotine effects on proliferation and the bomesin-like peptide autocrine system in human small cell lung carcinoma SHP77 cells in culture [J]. Lung Cancer 2000; 29:1–10.
Minna JD. Nicotine exposure and bronchial epithelial cell nicotinic acetylcholine receptor expression in the pathogenesis of lung cancer [J]. J Clin Invest 2003; 111:31–3.
Batori T, Sata M, Washida M, et al. Nicotine enhances neovascularization and promotes tumor growth [J]. Mol Cells 2003; 16:143–6.
Noui-Shirazi M, Guinet E. Evidence for the immunosuppressive role of nicotine on human dendritic cell factions [J]. Immunology 2003; 109:365–73.
Heusch WL, Maneckjee R. Signalling pathway involved in nicotine regulation of apoptosis of human lung cancer cells [J]. Carcinogenesis 1998; 19:551–6.
Mai H, May WS, Gao F, et al. A fuctional role for nicotine in Bc1–2 phosphorylation and suppression of of apoptosis [J]. J Biol Chem 2003; 278:1886–91.
Author information
Authors and Affiliations
Corresponding author
Additional information
Biography: XIE Zuo-fu (1957–), male, associate professor, Fujian Provincial Tumor Hospital, majors in cell and molecular biology of tumor.
Rights and permissions
About this article
Cite this article
Xie, Zf., Zheng, Tr., Lu, L. et al. Effect of nicotine on growth and expression of apoptosis-related genes of small cell lung cancer (SCLC) cell. Chin. J. Cancer Res. 17, 49–52 (2005). https://doi.org/10.1007/s11670-005-0010-9
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11670-005-0010-9