Abstract
Objective: To analyze the differentially expressed cDNA sequences related to chlorophyllin (CHL) mediated inhibition of malignant transformation of human bronchial epithelial cell line (16HBE). Methods: 16HBE cells treated with chlorophyllin and anti-BPDE were conducted as tester, 16HBE cells treated only with anti-BPDE were conducted as driver, and cDNA representational difference analysis (cDNA RDA) was used to compare the differential gene expression between the two kinds of cells. The cDNA fragments were ligated to pGEM-T vector and transformed into JM109 bacteria. The plasmid DNA was sequenced and compared with database in GenBank by BLASTN. Results: Among the 5 cloned cDNA sequences, three were novel and were registered in dbEST database, two showed sequence homology to alpha-enolase and a newly found gene ribosomal protein S18/S6-like. Conclusion: These 5 cDNA sequences might play important roles in antitransforming effect of chlorophyllin.
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Foundation item: This work was supported by the National Natural Science Foundation of China (No. 30000138); the Natural Science Foundation of Guangdong (No. 20011054).
Biography: ZHU Li-jin (1975–), female, master of medicine, assistant professor, Zhejiang Academy of Medical Science, majors in molecular toxicology.
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Zhu, Lj., Jiang, Yg. Isolation of human transcripts expressed in 16HBE cells related to chlorophyllin antitransforming activity against anti-BPDE by cDNA representational difference analysis. Chin. J. Cancer Res. 17, 6–10 (2005). https://doi.org/10.1007/s11670-005-0002-9
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DOI: https://doi.org/10.1007/s11670-005-0002-9
Key words
- Chlorophyllin
- Anti-benzo[a]pyrene diolepoxide
- Antitransformation
- Differential expression cDNA sequence
- Gene cloning