Abstract
Objective: To study the synergic effects of IL-12 and B7-1 transfectant on antitumor immunity in vivo. Methods: The retrovirus vector encoding mIL-12 and mB7-1 gene was tranfected into EL-4 thymic lymphoma cells respectively. The cells were used as tumor vaccine and the therapeutic effect was observed. Results: In contrast to the mice immunized with EL-4/Wt or EL-4/Neo groups, the tumorigenicity of EL-4/IL-12 transfectant was decreased (P<0.001). The EL-4/IL-12 and EL-4/B7-1 cells irradiated with 60Co showed significant systematic protective effects against the rechallenge of EL-4/Wt. 60Co irradiated EL-4/IL-12 cells delayed the occurrence of tumor and prolonged the survival period of tumor bearing mice. Combination of the vaccines of EL-4/IL-12 and EL-4/B7-1 resulted in the enhanced therapeutic effect compared with each single transfectant group (P<0.001). Conclusion: The results showed that IL-12 transduced cells could enhance the antitumor immunity of host as cancer vaccine. Combination of the EL-4/IL-12 and EL-4/B7-1 transfectant could improve immunity of host and is a prospect cancer vaccine.
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Foundation item: This work was supported by a grant from the National “95” Key Project of China (No.96-906A-01-20).
Biography: WANG Zhi-hua (1951–), male, professor, Institute of Oncology, Harbin Medical University, majors in tumor immunology and molecular biology.
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Wang, Zh., Li, H. & Zhang, Cy. Antitumor immunity and vaccine effect induced by IL-12 synergizes B7-1 gene transfected cells. Chin. J. Cancer Res. 15, 5–8 (2003). https://doi.org/10.1007/s11670-003-0002-6
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DOI: https://doi.org/10.1007/s11670-003-0002-6