Skip to main content
SpringerLink
Log in

Detection and its clinical value of minimal residual diseases in acute promyelocytic leukemia

  • Published:
Chinese Journal of Cancer Research

Abstract

Objective: To detect the minimal residual diseases (MRD) in acute promyelocytic leukemia (APL) after complete remission (CR) and to analyze its clinical value in prognosis. Methods: Reverse transcription Polymerase chain reaction (RT/PCR) was used to detect MRD of patients with APL. Results: MRD positive rate in patients with APL was 92.8% (39/42) before treatment and 56.7 (21/37) immediately after the ATRA or chemotherapy-induced CR. Furthermore, MRD positive rate was related to the relapse in APL patients and could be considered as a marker to predict the relapse of patients with APL after CR. The MRD detection could also be applied to direct the consolidation therapy to prevent relapses. Conclusion: RT-PCR is valuable to monitor MRD and can be used as a marker to predict relapses.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Baruchel A, Schaison G, Detection of minimal residual disease in leukemias[J]. Nouv Rev Fr Hematol 1990; 32: 21.

    PubMed  CAS  Google Scholar 

  2. Hoelzer D, Thiel E, Loffer H, et al. Prognostic factors in a multicenter study for treatment of acute lymphoblastic leukemia in adults[J]. Blood 1988; 71: 123.

    PubMed  CAS  Google Scholar 

  3. Nigrin RS, Blume KG. The use of polymerase chain reaction for the detection of minimal residual malignant disease[J]. Blood 1991; 78: 255.

    Google Scholar 

  4. Castaigne S, chomienne C, Daniel MT, et al. All-trans retinoic acid as a differentiation therapy for acute promyelocytic leukemia. I. Clinical results[J]. Blood 1990; 76: 1704.

    PubMed  CAS  Google Scholar 

  5. Chen SJ, Chen Z, Chen A, et al. Occurrence of distinct PML-RARα fusion gene isoforms in patients with acute promyelocytic leukemia detected by reverse transcriptase/polymerase chain reaction[J]. Oncogene 1992; 7: 1223.

    PubMed  CAS  Google Scholar 

  6. Pandlfi PP, Alcalay M, Fagioli M, et al. Genomic variability and alternative splicing generate multiple PML/RARα transcripts that encode aberrant PML proteins and PML/RARα isoforms in acute promyelocytic leukemia[J]. EMBO J 1992; 11: 1397.

    Google Scholar 

  7. Geng JP, Tong JH, Dong S, et al. Localization of the chromosome 15 breakpoints and expression of multiple PML/RARα transcripts in acute promyelocytic leukemia. A study of 28 Chinese patients[J]. Leukemia 1993; 7: 20.

    PubMed  CAS  Google Scholar 

  8. Lococo F, Diverio D, Pandolfi PP, et al. Molecular evaluation of residual disease as a prodictor of relapse in acute promyelocytic leukemia[J]. Lancet 1992; 340: 1437.

    Article  CAS  Google Scholar 

  9. Bennett JM, Catovsky d, Daniel MT, et al. Proposals for the classification of the acute leukemias. French-America-British (FAB) Coopration Group[J]. Br J Haematol 1976; 33: 451.

    PubMed  CAS  Google Scholar 

  10. Wang ZY, Sun GL, Lu JX, et al. Treatment of acute promyelocytic leukemia with all-trans retinoic acid in China[J]. Nouv Rev Fr hematol 1999; 32: 34.

    Google Scholar 

  11. Chomezynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenolchloroform extraction[J]. Anal Biochem 1987; 162: 156.

    Google Scholar 

  12. Viniou NA, Yataganas X, Stamatopoulos K, et al. Reverse transcription polymerase chain reaction for diagnosis and molecular monitoring of the PML-RARα fusion gene in acute promyelocytic leukemia[J]. Cancer Genet Cytogenet 1995; 84: 91.

    Article  PubMed  CAS  Google Scholar 

  13. Fenaux P, Chevret S, Guerci A, et al. Long-term follow-up conforms the benefit of all-trans retinoic acid in Acute promyelocytic leukemia. European APL group[J]. Leukemia 2000; 14: 1371.

    Article  PubMed  CAS  Google Scholar 

  14. Huang ME, Ye YC, Chen SR, et al. Use of all-trans retinoic acid in treatment of acute promyelocytic leukemia[J]. Blood 1988; 72: 567.

    PubMed  CAS  Google Scholar 

  15. Huang W, Sun GL, Li XS, et al. Acute promyelocytic leukemia: Clinical relevance of two major PML- RARα isoforms detection of minimal residual disease by retrotranscriptase/polymerase chain reaction to predict relapse[J]. Blood 1993; 82: 1264.

    PubMed  CAS  Google Scholar 

  16. Tobal K, Liu-yin JA, et al. RT-PCR method with increased sensitivity shows persistence of PML-RARα fusion transcripts in patients in log-term remission of APL[J]. Leukemia 1998; 12: 1349.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Hemato-Oncology, Shandong Academy of Medical Sciences, Shandong University, 250062, Jinan

    Jiang Guo-sheng, Tang Tian-hua, Zhang Yu-kun, Ren Hai-quan, Zhao Liang-yu, Ren Qing-hua, Jiang Feng-qin & Tian Zhi-gang

  2. Department of Hematology, Shandong Qian-Fe-Shan Hospital, 250014, Jinan

    Bi Ke-hong, Guo Gui-yue & Liu Xiu-lan

  3. Department of Hematology, Shandong University Qilu Hospital, 250012, Jinan

    Liu Chuan-fang & Peng Jun

Authors
  1. Jiang Guo-sheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tang Tian-hua
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Bi Ke-hong
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zhang Yu-kun
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ren Hai-quan
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Zhao Liang-yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Guo Gui-yue
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Liu Xiu-lan
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Ren Qing-hua
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Jiang Feng-qin
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Liu Chuan-fang
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Peng Jun
    View author publications

    You can also search for this author in PubMed Google Scholar

  13. Tian Zhi-gang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jiang Guo-sheng.

Additional information

Foundation item: This work was supported by the Natural Science Foundation of Shandong Province (No. 2000.11) and a grant from the Science & Technology Committee of Shandong Province (No. 951228104).

Biography: Jiang Guo-sheng(1964–), male, Ph.D, associate professor, Department of Hemo-Oncology, Shandong Academy of Medical Sciences, majors in hematology and tumor-biology.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jiang, Gs., Tang, Th., Bi, Kh. et al. Detection and its clinical value of minimal residual diseases in acute promyelocytic leukemia. Chin. J. Cancer Res. 14, 288–292 (2002). https://doi.org/10.1007/s11670-002-0063-y

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11670-002-0063-y

Key words

CLC number

Search

Navigation