Abstract
Object: To localize the mRNA coding for VEGF at Ultrastractural level in human breast cancer by using digoxigenin-labeled cDNA probes. Methods: Nonradioactive in situ hybridization at electron microscopic level was employed to detected VEGF mRNA in breast cancer. Result: Cancer cells and endothelial cell of angiogensis show dark color in experiment sections. No dark color can be found in control sections. Positive hybridization signals showed dark dot and were located in various compartments of the breast cancer cell and endothelial cell in experiment section. No labeling was observed in control sections. In experiment sections, the staining appeared concentrated in cytoplasm and nucleus of the breast cancer cell and endothelial cell. Conclusion: Nonradioactive in situ hybridization at electron microscopic level is efficient for direct observation of the target site mRNA of VEGF in the cytoplasm and nucleus.
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Foundation tem: This work was supported by National Natural Science Foundation of China (No. 39870314).
Biography: Wang Yi-shu(1972–), female, doctor of pathology, lecturer, Basic Medical College, Jilin University, majors in tumor angiogenesis.
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Wang, Ys., Li, YL., Wang, Xr. et al. Distribution of VEGF mRNA in breast cancer with nonradioactive in situ hybridization at electron microscopic levels. Chin. J. Cancer Res. 14, 270–273 (2002). https://doi.org/10.1007/s11670-002-0059-7
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DOI: https://doi.org/10.1007/s11670-002-0059-7