Skip to main content
SpringerLink
Log in

The effect of transfected Cx43 gene on the GJIC and proliferation of glioma cells

  • Articles
  • Published:
Chinese Journal of Cancer Research

Abstract

Objective: To evaluate the effect of Cx43 gene on gap junction intercellular communication (GJIC) and proliferation of glioma cells. Methods: Cx43 cDNA was transfected into TJ905 human glioblastoma cells using lipofectamine. The expression of Cx43 was identified by Northern blot analyses, in situ hybridization and immunohistochemistry. MTT assay and average number of AgNORs (Argyrophlic nuclear organizer regions) were used to determine the cell proliferation. TUNEL method was used for detection of cell apoptosis, and scrape loading and dye tranfer method for examination of GJIC. Results: The Cx43 expression was greatly upregulated when Cx43 gene was transfected into TJ905 glioma cells. The cell proliferation was inhibited while the cell apoptosis was not increased and GJIC was significantly restored in the glioma cells transfected with Cx43 gene. Conclusion: Cx43 gene has an inhibitory effect on the glioma cell proliferation, but no effect on induction of cell apoptosis. The restoration of GJIC may be the major mechanism involved in its effect. Cx43 gene can be the candidate for gene therapy of gliomas.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Omori Y, Yamasaki H. Mutated Connexin43 proteins inhibit rat glioma cells growth suppression mediated by wild-type connexin43 in a dominant-negative manner. Int J Cancer 1998; 78: 446.

    Article  PubMed  CAS  Google Scholar 

  2. Dermietzel R, Spray DC. Gap junctions in the brain: where, what type, how many and why? TINS 1993; 16: 186.

    PubMed  CAS  Google Scholar 

  3. Holder JW, Elmore E, Barrett JC. Gap junction function and cancer. Cancer Res 1993; 53: 3475.

    PubMed  CAS  Google Scholar 

  4. Goldberg GS, Bechberger JF, Tajima Y, et al. Connexin43 suppresses MFG-E8 while enhancing contact growth inhibition of glioma cells. Cancer Res 2000; 60: 6018.

    PubMed  CAS  Google Scholar 

  5. Trosko JE, Ruch RJ. Cell-cell communication in carcinogenesis. Front Biosci 1998; 3: 208.

    Google Scholar 

  6. Zhang W, Couldwell WT, Simard mF, et al. Direct gap junction communication between malignant glioma cells and astrocytes. Cancer Res 1999; 59: 1994.

    PubMed  CAS  Google Scholar 

  7. Hoz- Wagenblatt, Shalloway D. Gap junctional communication and neoplastic transformation. Crit Rev Oncog 1993; 4: 541.

    Google Scholar 

  8. Shinoura N, Chen L, Wast MA, et al. Protein and messenger RNA expression of connexin43 in astrocytomas: implications in brain tumor gene therapy. J Neurosurg 1996; 84: 839.

    PubMed  CAS  Google Scholar 

  9. Xia ZB, Pu PY, Wang CY, et al. Connexin43 gene expression in brain tumors originating from the neuroepithelial tissues. Chin J Nervous Mental Dis 2000; 26: 324.

    Google Scholar 

  10. Soroceanu L, Manning TJ jr, Sontheimer H. Reduced expression of connexin43 and functional gap junction coupling in human gliomas. Glia 2001; 33: 107.

    Article  PubMed  CAS  Google Scholar 

  11. Pu PY, Liu XW, Liu AX, et al. Inhibitory effect of antisense epidermal growth factor receptor RNA in the proliferation of rat C6 glioma cells in vitro and in vivo. J Neurosurg 2000; 92: 132.

    Article  PubMed  CAS  Google Scholar 

  12. Singh MV, Bhatnage R, Price CJ, et al. Gap junctions in 9L and C6 glioma cells: Correlation with growth characteristics. Cytobios 1997; 89: 209.

    PubMed  CAS  Google Scholar 

  13. Huang RP, Fan Y, Mohammad Z, et al. Reversion of the neoplastic phenotype of human glioblastoma cells by Connexin43 (Cx43). Cancer Res 1998; 58: 5089.

    PubMed  CAS  Google Scholar 

  14. Bradshaw SI, Naus CCG, Zhu D, et al. Alterations in the synthesis of insulin-like growth factor binding proteins and insulin-like growth factors in rat C6 glioma cells transfected with a gap junction connexin43 cDNA. Regul Pept 1993; 48: 99.

    Article  PubMed  CAS  Google Scholar 

  15. Chen SC, Pelletier DB, Ao P, et al. Connexin43 reverses the phenotype of transformed cells and alters their expression of cyclin/cyclin-dependent kinases. Cell Growth Differ 1995; 6: 681.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Neurosurgery, Tianjin Medical University General Hospital and Laboratory of Neuro-oncology, Tianjin Neurological Institute, 300052, Tianjin

    Pu Pei-yu, Xia Zhi-bo, Huang Qiang, Wang Chun-yan & Wang Guang-xiu

Authors
  1. Pu Pei-yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xia Zhi-bo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Huang Qiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Wang Chun-yan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Wang Guang-xiu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Pu Pei-yu.

Additional information

Foundation item: This work was supported by the National Natural Science Foundation of China (No. 39870815).

Biography: Pu Pei-yu(1933–), Professor, Department of Neurosurgery, Tianjin Medical University, majors in neurosurgery and neuro-oncology.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pu, Py., Xia, Zb., Huang, Q. et al. The effect of transfected Cx43 gene on the GJIC and proliferation of glioma cells. Chin. J. Cancer Res. 14, 100–104 (2002). https://doi.org/10.1007/s11670-002-0022-7

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11670-002-0022-7

Key words

CLC number

Search

Navigation