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A study on the treatment of gastric cancer by CD gene combined with 5-FC in vitro and in vivo

  • Clinical Observation
  • Published:
Chinese Journal of Cancer Research

Abstract

Objective: To study the killing effect of suicide gene CD on mouse gastric cancer. Methods: CD gene was transduced with the retroviral vector. The killing effect and bystander effect of CD gene on mouse gastric cancer cell line MFC were observed. The mouse gastric cancer model was used for in vivo study. The CD gene containing virus was injected into the tumors. The volumes of the tumors in every group were measured in time. Results: Significant killing effect and bystander effect were observed by CD gene in vitro, 70–80% cell death resulting from 20% of CD gene transduction. In vivo, CD/5-Fc caused tumor to diminution. Conclusion: CD/5-Fc system has significant killing effect on mouse gastric cancer

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References

  1. Huber BE, Richards CA, Krenisky TA. Retroviral mediated gene therapy for the treatment of hepatocellular carcinoma: An innovative approach for cancer therapy[J]. Proc Natl Acad Sci USA 1991; 88: 8039.

    Article  PubMed  CAS  Google Scholar 

  2. Freeman SM, Abond CN, Whatenby KA et al. The “bystander effect”: tumor regression when a fraction of tumor mass is genetically modified[J]. Cancer Res 1993; 53:5274.

    PubMed  CAS  Google Scholar 

  3. Moolten FC. Tumor chemosensitivity conferred by inserted herpes thymidine kinase genes; paradigm for a prospective cancer control strategy[J]. Cancer Res 1996; 46: 4276.

    Google Scholar 

  4. Mullen CA, Coale MM, Loue R, et al. Tumor expressing the cytosine deaminase suicide gene can be eliminated in vivo with 5-Fc and induce protective immunity to wild type tumor[J]. Cancer Res 1994; 54: 1503.

    PubMed  CAS  Google Scholar 

  5. Fick J, Barker FG, II Dazin P, et al. The extent of heterocellular communication mediated by gap junctions is predictive of bystander tumor cytotoxicity in vitro[J]. Proc Natl Acad Sci USA 1995; 92:11071.

    Article  PubMed  CAS  Google Scholar 

  6. Freeman SM, Ramesh R, Marogi AJ. Immune system in suicide gene therapy[J]. Lancet 1997; 349: 2.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Surgery, The Affiliated Shanghai Ninth People’s Hospital, Shanghai Second Medical University, 20001, Shanghai

    Guo Shan-yu

  2. Department of Surgery, Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Second Medical University, 200025, Shanghai

    Gu Qin-long, Zhu Zheng-gang & Lin Yanzhen

Authors
  1. Guo Shan-yu
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  2. Gu Qin-long
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  3. Zhu Zheng-gang
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  4. Lin Yanzhen
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Corresponding author

Correspondence to Guo Shan-yu.

Additional information

Foundation item: This work was supported by the Scientific and Research Foundation of the Ministry of Public health of China(No. 98-1-312) and Shanghai Natural Scientific Foundation (No. 98ZB14028).

Biography: GUO Shan-yu(1966–), doctor of medicine, attending physician, Shanghai Ninth People’s Hospital, majors in biological therapy of gastrointestinal cancer.

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Guo, Sy., Gu, Ql., Zhu, Zg. et al. A study on the treatment of gastric cancer by CD gene combined with 5-FC in vitro and in vivo . Chin. J. Cancer Res. 13, 294–297 (2001). https://doi.org/10.1007/s11670-001-0051-7

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  • DOI: https://doi.org/10.1007/s11670-001-0051-7

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