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Construction and expression of a human-mouse chimeric antibody against human bladder cancer

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Chinese Journal of Cancer Research

Abstract

Objective: To construct and express a human-mouse chimeric antibody against human bladder cancer. Method: The variable region genes of anti-human bladder cancer monoclonal antibody BDI-1 were cloned by RT-PCR. A human-mouse chimeric antibody expression vector was constructed and transfected into CHO cells. The chimeric antibody against bladder cancer was expressed and characterized. Result: Eukaryotic expression vector of the chimeric antibody against human bladder carcinoma was successfully constructed, and was expressed in eukaryotic cells; the expressed chimeric antibody ch-BDI showed same specificity as its parent McAb against human bladder cancer cells. Conclusion: The constructed chimeric antibody was expressed successfully in eukaryotic cells, and the chimeric antibody had desired affinity against human bladder cancer cells.

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Authors and Affiliations

  1. Central Laboratory, Naval General Hospital, 100037, Beijing

    Bai Yin, Wang Yan & Zhou Li-Jun

  2. Institute of Surgical Urology, The First Hospital, Peking University, 100034, Beijing

    Bai Yin & Yu Li-Zhang

Authors
  1. Bai Yin
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  2. Wang Yan
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  3. Zhou Li-Jun
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  4. Yu Li-Zhang
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Corresponding author

Correspondence to Wang Yan.

Additional information

Foundation item: This work was supported by the National Natural Science Foundation of China(No. 30070706).

Biography: BAI Yin(1971–), doctoral candidate, the First Hospital, Peking University, majors in Urology.

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Bai, Y., Wang, Y., Zhou, LJ. et al. Construction and expression of a human-mouse chimeric antibody against human bladder cancer. Chin. J. Cancer Res. 13, 271–275 (2001). https://doi.org/10.1007/s11670-001-0046-4

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  • DOI: https://doi.org/10.1007/s11670-001-0046-4

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