Abstract
Objective: To investigate the influence of consecutive immunization on cellular and humoral immunity in mice. Methods: We evaluated a consecutive immunization strategy of priming with recombinant fowlpox virus vUTALG and boosting with plasmid DNA pcDNAG encoding HIV-1 capsid protein Gag. Results: In immunized mice, the number of CD +4 T cells from splenic lymphocytes increased significantly and the proliferation response of splenocytes to ConA and LPS elevated markedly and HIV-1-specific antibody response could be induced. Conclusion: Consecutive immunization could increase cellular and humoral immunity responses in mice.
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Foundation item: This work was supported by the National Scientific Research Foundation for Excellent Young Scientist of China (No. 398251197).
Biography: Luo Kun(1966–), doctor of medicine, lecturer, now works at Institute of Immunology, Zhejiang University, majors in molecular immunology.
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Luo, K., Jin, Ny., Guo, Zr. et al. Consecutive immunization with recombinant fowlpox virus and plasmid DNA for enhancing cellular and humoral immunity. Chin. J. Cancer Res. 13, 247–250 (2001). https://doi.org/10.1007/s11670-001-0040-x
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DOI: https://doi.org/10.1007/s11670-001-0040-x