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Electro-Immobilization of Acetylcholinesterase Using Polydopamine for Carbaryl Microsensor

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Abstract

A simple and sensitive electrochemical acetylcholinesterase (AChE) biosensor for determination of carbaryl, one of the most commonly used carbamate pesticides, is described. The AChE enzyme was successfully entrapped by a polydopamine–graphene composite on polypyrrole nanowires that modified interdigitated planar platinum-film microelectrodes . The influence of different parameters on the operation of the biosensor was also studied. The selected parameters for the biosensor performance in detecting carbaryl were as follows: applied potential + 0.7 V, pH 7.4 at 25°C. The inhibition of carbaryl was proportional to its concentrations ranging from 0.05 to 1.5 μg/mL with the detection limit of 0.008 μg/mL using chronoamperometry. This study provides a promising approach in fabrication of sensitive biosensors for the analysis of carbamate pesticides as well as other hazardous compounds.

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Acknowledgements

This work was financially supported by the Ministry of Education and Training (Vietnam) [Grant Code B2014-01-65] and the Vietnam National Foundation for Science and Technology [Grant Code NAFOSTED 104.03-2013.52].

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Authors and Affiliations

  1. School of Chemical Engineering, Hanoi University of Science and Technology, 1 Dai Co Viet Road, Hanoi, Vietnam

    Trung B. Ha, Huyen T. Le, Ha H. Cao, Huy L. Nguyen & Vân-Anh Nguyen

  2. Institute of Applied Physics and Scientific Instrument, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Hanoi, Vietnam

    Nguyen Thanh Binh

  3. Hanoi National University of Education, 136 Xuan Thuy, Cau Giay, Hanoi, Vietnam

    Le Hai Dang

  4. Research Centre for Environmental Technology and Sustainable Development, Hanoi University of Science, 334 Nguyen Trai Road, Hanoi, Vietnam

    Quan P. Do

  5. Institute for Tropical Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Hanoi, Vietnam

    Dzung T. Nguyen

  6. Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Hanoi, Vietnam

    Tran Dai Lam

  7. Center for High Technology Development, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Hanoi, Vietnam

    Tran Dai Lam

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  1. Trung B. Ha
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  2. Huyen T. Le
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Correspondence to Huy L. Nguyen, Tran Dai Lam or Vân-Anh Nguyen.

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Ha, T.B., Le, H.T., Cao, H.H. et al. Electro-Immobilization of Acetylcholinesterase Using Polydopamine for Carbaryl Microsensor. J. Electron. Mater. 47, 1686–1693 (2018). https://doi.org/10.1007/s11664-017-5880-3

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  • DOI: https://doi.org/10.1007/s11664-017-5880-3

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