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Electrochemical Immunosensor Based on Fe3O4/PANI/AuNP Detecting Interface for Carcinoembryonic Antigen Biomarker

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Abstract

A low-cost screen-printed carbon electrode (SPCE) modified with Fe3O4, gold nanoparticles (AuNPs), and polyaniline (PANI) has been developed for rapid measurement of carcinoembryonic antigen (CEA) biomarker. The electrode surface was covered with Fe3O4 nanoparticles by drop coating then with PANI via electropolymerization. The resulting surface was further modified by AuNPs via electrodeposition. Key factors affecting the electrochemical behavior and sensing performance of the electrode were investigated. The results demonstrated that Fe3O4 mass loading of 2 mg/cm2 and 15 cycles of PANI polymerization were optimal for electrochemical measurement of CEA biomarker. In addition, compared with bare SPCE, coating the electrode surface with PANI, Fe3O4/PANI, and Fe3O4/PANI/AuNP significantly enhanced the peak oxidation current by approximately 16%, 52%, and 93%, respectively. The sensors exhibited linear trends with CEA concentration from 0 ng/mL to 10 ng/mL. The limit of detection and sensitivity of the electrode were estimated to be 0.25 ng/mL and 0.3827 μA/ng mL−1, respectively. Such sensors could be easily integrated into microfluidic platforms and could serve as a low-cost, rapid, point-of-care measurement method for CEA cancer biomarker.

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Authors and Affiliations

  1. Asian Institute of Technology, 58 Moo 9, Paholyothin Highway, Klong Luang, Pathumthani, 12120, Thailand

    Chamali Amarasiri & Loc Thai Nguyen

  2. Institute of Applied Physics and Scientific Instrument, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam

    Thanh Binh Nguyen

  3. University of Science and Technology of Hanoi, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam

    Vu Thi Thu

  4. Center for High Technology Development, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam

    Vu Thi Thu & Tran Dai Lam

  5. Bach Mai Hospital, 78 Giai Phong, Hai Ba Trung, Hanoi, Vietnam

    Nguyen Thi My Thuy

  6. Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam

    Tran Dai Lam

Authors
  1. Chamali Amarasiri
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  2. Thanh Binh Nguyen
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  3. Loc Thai Nguyen
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  4. Vu Thi Thu
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  5. Nguyen Thi My Thuy
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  6. Tran Dai Lam
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Corresponding authors

Correspondence to Thanh Binh Nguyen, Loc Thai Nguyen or Tran Dai Lam.

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Amarasiri, C., Nguyen, T.B., Nguyen, L.T. et al. Electrochemical Immunosensor Based on Fe3O4/PANI/AuNP Detecting Interface for Carcinoembryonic Antigen Biomarker. J. Electron. Mater. 46, 5755–5763 (2017). https://doi.org/10.1007/s11664-017-5610-x

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  • DOI: https://doi.org/10.1007/s11664-017-5610-x

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