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Biochip for Real-Time Monitoring of Hepatitis B Virus (HBV) by Combined Loop-Mediated Isothermal Amplification and Solution-Phase Electrochemical Detection

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Abstract

Accurate in situ diagnostic tests play a key role in patient management and control of most infectious diseases. To achieve this, use of handheld biochips that implement sample handling, sample analysis, and result readout together is an ideal approach. We present herein a fluid-handling biochip for real-time electrochemical monitoring of nucleic acid amplification based on loop-mediated isothermal amplification and real-time electrochemical detection on a microfluidic platform. Intercalation between amplifying DNA and free redox probe in solution phase was used to monitor the number of DNA copies. The whole diagnostic process is completed within 70 min. Our platform offers a fast and easy tool for quantification of viral pathogens in shorter time and with limited risk of all potential forms of cross-contamination. Such diagnostic tools have potential to make a huge difference to the lives of millions of people worldwide.

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Acknowledgements

This research is financially supported by NAFOSTED (Grant No. 104.04-2014.36). This research was partly funded by USTH (Grant NANO 1). We are grateful for the kind help from Dr. Le Thi Hoi from the National Hospital of Tropical Diseases for providing the samples from clinical sputum specimens.

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Authors and Affiliations

  1. Military Academy of Logistics, Ngoc Thuy, Long Bien, Hanoi, Vietnam

    Bui Quang Tien

  2. University of Science and Technology of Hanoi, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam

    Nguyen Thy Ngoc &  Vu Thi Thu

  3. Asian Institute of Technology, 58 Moo 9, Paholyothin Highway Klong Luang, Pathumthani, 12120, Thailand

    Nguyen Thai Loc

  4. Center for High Technology Development, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam

    Bui Quang Tien,  Vu Thi Thu & Tran Dai Lam

  5. Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam

    Tran Dai Lam

Authors
  1. Bui Quang Tien
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  2. Nguyen Thy Ngoc
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  3. Nguyen Thai Loc
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  4. Vu Thi Thu
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  5. Tran Dai Lam
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Corresponding authors

Correspondence to Vu Thi Thu or Tran Dai Lam.

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Tien, B.Q., Ngoc, N.T., Loc, N.T. et al. Biochip for Real-Time Monitoring of Hepatitis B Virus (HBV) by Combined Loop-Mediated Isothermal Amplification and Solution-Phase Electrochemical Detection. J. Electron. Mater. 46, 3565–3571 (2017). https://doi.org/10.1007/s11664-017-5483-z

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  • DOI: https://doi.org/10.1007/s11664-017-5483-z

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