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Inflammatory and Immunomodulatory Effects of Tripterygium wilfordii Multiglycoside in Mouse Models of Psoriasis Keratinocytes

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Abstract

Objective

To determine the role of Tripterygium wilfordii multiglycoside (TGW) in the treatment of psoriatic dermatitis from a cellular immunological perspective.

Methods

Mouse models of psoriatic dermatitis were established by imiquimod (IMQ). Twelve male BALB/c mice were assigned to IMQ or IMQ+TGW groups according to a random number table. Histopathological changes in vivo were assessed by hematoxylin and eosin staining. Ratios of immune cells and cytokines in mice, as well as PAM212 cell proliferation in vitro were assessed by flow cytometry. Pro-inflammatory cytokine expression was determined using reverse transcription quantitative polymerase chain reaction.

Results

TGW significantly ameliorated the severity of IMQ-induced psoriasis-like mouse skin lesions and restrained the activation of CD45+ cells, neutrophils and T lymphocytes (all P<0.01). Moreover, TGW significantly attenuated keratinocytes (KCs) proliferation and downregulated the mRNA levels of inflammatory cytokines including interleukin (IL)-17A, IL-23, tumor necrosis factor α, and chemokine (C-X-C motif) ligand 1 (P<0.01 or P<0.05). Furthermore, it reduced the number of γ δ T17 cells in skin lesion of mice and draining lymph nodes (P<0.01).

Conclusions

TGW improved psoriasis-like inflammation by inhibiting KCs proliferation, as well as the associated immune cells and cytokine expression. It inhibited IL-17 secretion from γ δ T cells, which improved the immune-inflammatory microenvironment of psoriasis.

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Author information

Authors and Affiliations

  1. Department of Dermatology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, 200437, China

    Shuo Zhang, Hong-jin Li, Chun-mei Yang, Liu Liu, Xiao-ying Sun, Jiao Wang, Si-ting Chen, Yi Lu, Man-qi Hu, Ge Yan, Ya-qiong Zhou, Xiao Miao & Xin Li

  2. Institute of Dermatology, Shanghai Academy of Traditional Chinese Medicine, Shanghai, 201203, China

    Shuo Zhang, Hong-jin Li, Chun-mei Yang, Liu Liu, Xiao-ying Sun, Jiao Wang, Si-ting Chen, Yi Lu, Man-qi Hu, Ge Yan, Ya-qiong Zhou, Xiao Miao, Xin Li & Bin Li

  3. Department of Integrated Traditional Chinese and Western Medicine, Shanghai Skin Disease Hospital, Tongji University School of Medicine, Shanghai, 200443, China

    Bin Li

Authors
  1. Shuo Zhang
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  2. Hong-jin Li
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  4. Liu Liu
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  8. Yi Lu
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  10. Ge Yan
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  13. Xin Li
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  14. Bin Li
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Contributions

Zhang S contributed to validation, formal analysis and wrote original draft. Zhang S, Li HJ, Liu L and Sun XY carried out the experiments. Li HJ contributed to methodology and visualization of study. Yang CM, Wang J, Yan G and Zhou YQ performed formal analysis. Sun XY and Miao X performed validation. Lu Y and Hu MQ contributed to visualization. Liu L, Chen ST and Li X contributed to review and editing of manuscript. Li X was responsible for resources and funding acquisition. Li B contributed to conceptualization, resources, and project administration. All authors have read and approved the final manuscript.

Corresponding author

Correspondence to Xin Li.

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There are no conflicts of interest to declare.

Additional information

Supported by the National Natural Science Foundation of China (Nos. 81973860, 82074427), Shanghai Pujiang Talent Program (No. 2020PJD067), and Science and Technology Commission of Shanghai Municipality (Nos. 21Y21920100, 21Y21920102)

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Zhang, S., Li, Hj., Yang, Cm. et al. Inflammatory and Immunomodulatory Effects of Tripterygium wilfordii Multiglycoside in Mouse Models of Psoriasis Keratinocytes. Chin. J. Integr. Med. 30, 222–229 (2024). https://doi.org/10.1007/s11655-023-3599-y

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  • DOI: https://doi.org/10.1007/s11655-023-3599-y

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