Abstract
Objective
To investigate and reveal the underlying mechanism of the effect of total saponins from Dioscoreae nipponica Makino (TSDN) on the arachidonic acid pathway in monosodium urate (MSU) crystal-induced M1-polarized macrophages.
Methods
M1 polarization of RAW264.7 cells were induced by 1 µ g/mL lipopolysaccharide (LPS). The methylthiazolyldiphenyl-tetrazolium bromide method was then used to screen the concentration of TSDN. MSU (500 µ g/mL) was used to induce the gouty arthritis model. Afterwards, 10 µ g/L TSDN and 8 µ mol/L celecoxib, which was used as a positive control, were added to the above LPS and MSU-induced cells for 24 h. The mRNA and protein expressions of cyclooxygenase (COX) 2, 5-lipoxygenase (5-LOX), microsomal prostaglandin E synthase derived eicosanoids (mPGES)-1, leukotriene B (LTB)4, cytochrome P450 (CYP) 4A, and prostaglandin E2 (PGE2) were tested by real-time polymerase chain reaction and Western blotting, respectively. The enzyme-linked immunosorbent assay was used to test the contents of M1 markers, including inducible nitric oxid synthase (NOS) 2, CD80, and CD86.
Results
TSDN inhibited the proliferation of M1 macrophages and decreased both the mRNA and protein expressions of COX2, 5-LOX, CYP4A, LTB4, and PGE2 (P<0.01) while increased the mRNA and protein expression of mPGES-1 (P<0.05 or P<0.01). TSDN could also significantly decrease the contents of NOS2, CD80, and CD86 (P<0.01).
Conclusion
TSDN has an anti-inflammation effect on gouty arthritis in an in vitro model by regulating arachidonic acid signaling pathway.
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Zhou Q designed and carried out the experiment and wrote this paper, Sun HJ carried part of the experiment, and Liu SM helped writing this paper.
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Supported by Joint Guidance Project of Heilongjiang Natural Science Foundation (No. LH2021H099), Start Fund of Postdoctoral Research in Heilongjiang Province (No. LBH-Q19188), Outstanding Youth Development Fund of Heilongjiang University of Chinese Medicine (No. 2019JC06), Project of Heilongjiang Administration of Traditional Chinese Medicine (No. ZHY202094), Graduate Innovative Scientific Research Project of Heilongjiang University of Chinese Medicine (No. 2020yjscx057), Natural Science Foundation of Heilongjiang Province (Key project, No. ZD2020H006)
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Zhou, Q., Sun, Hj. & Liu, Sm. Effects of Total Saponins from Dioscorea Nipponica Makino on Monosodium Urate-Induced M1-Polarized Macrophages through Arachidonic Acid Signaling Pathway: An in vitro Study. Chin. J. Integr. Med. 29, 44–51 (2023). https://doi.org/10.1007/s11655-022-3721-6
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DOI: https://doi.org/10.1007/s11655-022-3721-6