Abstract
Objective
To verrify the anti-tumor efficacy and toxicity between juglone (Jug) and Jug-loaded PLGA nanoparticles (Jug-PLGA-NPs).
Methods
Jug-PLGA-NPs were prepared by ultrasonic emulsification. The anti-tumor activity of Jug (2, 3, 4 µg/mL) and Jug-PLGA-NPs (Jug: 2, 3, 4 µg/mL) in vitro was measured by MTT assay and cell apoptosis analysis. The distribution, anti-tumor effect and biological safety in vivo was evaluated on A375 nude mice.
Results
With the advantage of good penetration and targeting properties, Jug-PLGA-NPs significantly inhibited proliferation and migration of melanoma cells both in vitro and in vivo (P<0.05 or P<0.01) with acceptable biocompatibility.
Conclusions
Jug can inhibit the growth of melanoma but is highly toxic. With the advantage of sustained release, tumor targeting, anti-tumor activity and acceptable biological safety, Jug-PLGA-NPs provide a new pharmaceutical form for future application of Jug.
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Yue WH and Zou ZY conceived the idea and designed the experiments. Yue WH and Qin LQ performed the experiments and interpreted the experimental results. Cai J, Mei R and Qian HQ participated in the study design and data analysis. Yue WH, Qin LQ and Zou ZY wrote the manuscript. All authors read and approved the current version of the manuscript.
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The authors declared no potential conflicts of interest concerning the research, authorship, and/or publication of this article.
Supported by the National Natural Science Foundation of China (Nos. 81872484 and 82073365) and the Social Development Fund of Jiangsu Province, China (No. BE2019605)
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Yue, Wh., Qin, Lq., Cai, J. et al. Jug-PLGA-NPs, a New Form of Juglone with Enhanced Efficiency and Reduced Toxicity on Melanoma. Chin. J. Integr. Med. 28, 909–917 (2022). https://doi.org/10.1007/s11655-021-3461-y
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DOI: https://doi.org/10.1007/s11655-021-3461-y